Slime production and cell surface hydrophobicity of nasopharyngeal and skin staphylococci isolated from healthy people

• Autorzy: Malm A , Biernasiuk A. , Los R. , Kosikowska U. , Juda M. , Korona-Glowniak I. , Gorniewski G.
• Języki publikacji: EN

Abstrakty

EN

The collection of 314 staphylococcal strains including Staphylococcus aureus and coagulase-negative staphylococci (CNS) was isolated from skin or nasopharynx of healthy people. It was found that the majority of staphylococci possessed the ability to produce slime intensively or moderately, irrespective of ecological niche-nose, throat or skin. Most of them showed the hydrophilic cell surface. However, among S. aureus skin isolates or CNS throat isolates predominated strains with hydrophobic cell surface. There was a slight correlation between slime production and the nature of cell surface among CNS isolates but not among S. aureus strains. It was found that most of slime-producing CNS strains showed hydrophilic cell surface, while slime-negative isolates usually possessed hydrophobic cell surface. Our data suggest that slime production but not cell surface hydrophobicity can be regarded as an essential colonization factor responsible for staphylococci adherence to skin or mucous membranes of upper respiratory tract. These data also suggest that slime production seems to be a general feature of staphylococci isolated from various niches of healthy people.

Słowa kluczowe

EN

cell surface; hydrophobicity; adhesion; slime production; bacterial cell; Staphylococcus aureus; microbiology; healthy people

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2005
• Tom: 54
• Numer: 2
• Opis fizyczny: p.117-121,fig.,ref.

Twórcy

autor

Malm A

• Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland

autor

Biernasiuk A.

autor

Los R.

autor

Kosikowska U.

autor

Juda M.

autor

Korona-Glowniak I.

autor

Gorniewski G.

Bibliografia

• Ammendolia M.G., R. Di Rosa, L. Montanaro, C.R. Arciola and L. Baldassarri. 1999. Slime production and expression of the slime-associated antigen by staphylococcal clinical isolates. J. Clin. Microbiol. 37: 3235-3238.
• Baldassarri L., G. Donelli, A. Gelosia, A.W. Simpson and G.D. Christensen. 1997. Expression of slime interferes with in vitro detection of host protein receptors of Staphylococcus epidermic/is. Infect. Immun. 65: 1522-1526.
• Costerton J.W., P.S. Stewart and E.P. Greenberg. 1999. Bacterial biofilms: a common cause of persistent infections. Science. 284: 1318-1322.
• Cree R.G.A., P. Aleljung, M. Paulsson, W. Witte, W.C. Noble, A. Ljungh and T. Wadstrom. 1994. Cell surface hydrophobicity and adherence to extra-cellular matrix proteins in two collections of methicillin-resistant Staphylococcus aureus. Epidemiol. Infect. 112: 307-314.
• Cuny C, G. Werner, C. Braukle, I. Klare and W. Witte. 2002. Diagnostics of staphylococci with special reference to MRSA. J. Lab. Med. 26: 165-173.
• Donlan R.M. and J.W. Costerton. 2002. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev. 15: 167-193.
• Drago L., De Vecchi, M. Valli, L. Nicola and M.R. Gismondo. 2002. Effect of linezolid in comparison with that of vancomycin on glycocalix production: in vitro study. Antimicroh. Agents Chemother. 46: 598-599.
• von Eiff C., C. Heilmann and G. Peters. 1999. New aspects in the molecular basis of polymer-associated infections due to staphylococci. Eur. J. Clin. Microbiol. Infect. Dis. 18: 843-846.
• Freeman D.J., F.R. Falkiner and C.T. Keanc. 1989. New method for detecting slime production by coagulase negative staphylococci. J. Clin. Pathol. 42: 872-874.
• Howard B.J. and W.E. Kloos. 1994. Staphylococci, p. 243-256. In: Howard B.J., Keiser J.F., Smith T.F., Weissfeld A.S., Tilton R.C. (eds.), Clinical and pathogenic microbiology. Mosby, St. Louis.
• Howard B.J. and J.C. Rees. 1994. Host-parasite interactions: mechanisms of pathogenicity, p. 9-36. In: Howard B.J., Keiser J.F., Smith T.F., Weissfeld A.S., Tilton R.C. (eds.), Clinical and pathogenic microbiology. Mosby, St. Louis.
• Janicka G., A. Mikucka, A. Sękowska, T. Zwierzchlewski and M. Wróblewski. 2002. Autoaggregation, hydrophobic, and hydrophilic properties of Moraxella catarrhalis strains. Acta Microbiol. Polon. 51: 23-30.
• Jankowski S., J. Sarowska, H. Żarczyńska and A. Cisowska. 1997. Hydrophobic properties ofPseudomonas aeruginosa strains (in Polish). Med. Dośw. Mikrobiol. 49: 187-190.
• Lindahl M., A. Faris, T. Wadstrom and S. Hjerten. 1981. A new test based on "salting out" to measure relative surface hydrophobicity of bacterial cells. Biochim. Biophys. Acta. 677: 471-476.
• Łoś R., A. Malm, A. Biernasiuk, I. Korona-GIowniak and U. Kosikowska. 2004. Hydrophobic proporties of Gram-negative rods colonizing upper respiratory tract of healthy people (in Polish). Med. Dośw. Mikrobiol. 56: 57-65.
• Mikucka A., E. Gospodarek and B. Ulatowska. 2000. Influence of growth conditions on cell surface hydrophobicity of rods of genus Serratia (in Polish). Med. Dośw. Mikrobiol. 52: 9-15.
• Shuter J., V.B. Hatcher and F.D. Lowy. 1996. Staphylococcus aureus binding to human nasal mucin. Infect. Immun. 64: 310-318.
• Wilson R., R.B. Dowling and A.D. Jackson. 1996. The biology of bacterial colonization and invasion of the respiratory mucosa. Eur. Respir. J. 9: 1523-1530.
• Wu J.A., C. Kusuma, J.J. Mond and J.F. Kokai-Kun. 2003. Lysostaphin disrupts Staphylococcus aureus and Staphylococcus epidermidis biofilms on artificial surfaces. Antimicrob. Agents Chemother. 47: 3407-3414.