The use cytoplasmic markers in onion hybrid breeding

• Autorzy: Szklarczyk M , Simlat M. , Jagosz B. , Ba G.
• Języki publikacji: EN

Abstrakty

EN

We applied the RFLP approach to identify the cytoplasmic genotypes of selected onion breeding materials from Poland. For this purpose, mitochondrial DNA from cytoplasmic male-sterile (CMS) and male-fertile onions were hybridized with the probes for the following mitochondrial genes: atpA, atp6, atp9, cob, cox1, nad3, nad4 and nad6. S-, T- or C-cytoplasm was represented in each analyzed sterile accession. Some new polymorphisms shared by S- and C-cytoplasmic onions were identified. We also used currently available PCR markers to test if cytoplasmic heterogenity occurs within onion inbreds. A fraction of the plants bearing S-cytoplasm were found within two male-fertile lines, but such plants were not detected in the open-pollinated cultivars Sochaczewska, Wolska and Żytawska. Both the RFLP and PCR approaches gave some proof of existing mitochondrial heteroplasmy in onions.

Słowa kluczowe

EN

cytoplasmic marker; Allium cepa; mitochondrial DNA; hybrid seed; hybrid; mtDNA; seed production; onion; plant breeding

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2B
• Opis fizyczny: p.625-634,fig.

Twórcy

autor

Szklarczyk M

• Agricultural University of Krakow, 29 Listopada 54, 31-425 Krakow, Poland

autor

Simlat M.

autor

Jagosz B.

autor

Ba G.

Bibliografia

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