Evaluation of apoptosis, proliferation intensity and metallothionein [MT] expression in comparison with selected clinicopathological variables in primary adenocarcinomas of the large intestine

• Autorzy: Dumanska M , Dziegiel P. , Sopel M. , Wojnar A. , Zabel M.
• Języki publikacji: EN

Abstrakty

EN

Tumour growth and expansion are the result of proliferative activity and the capacity to eliminate cells by apoptosis and/or necrosis. The present study was aimed at comparing the apoptosis and proliferation intensity in cells of adenocarcinomas of the large intestine with the expression of metallothionein (MT), the grade of the tumour and the depth to which the tumour infiltrated the intestinal wall. The TUNEL technique and immunocytochemical reactions (expression of caspase-3, Ki-67, MT) were used to detect apoptosis. The results demonstrated augmented levels of all the variables examined, positively correlated with grade of malignancy, G, and with the depth of intestinal wall infiltration by the tumour cells. The testing of apoptosis, proliferation and MT expression may prove useful in the appraisal of the growth and progression of primary adenocarcinomas in the large intestine.

Słowa kluczowe

EN

proliferation; malignant tumour; Ki-67 antigen; adenocarcinoma; metallothionein; large intestine; tumour; expression; apoptosis

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2004
• Tom: 63
• Numer: 1
• Opis fizyczny: p.107-110,fig.,ref.

Twórcy

autor

Dumanska M

• University School of Medicine, Chalubinskiego 6a, 50-368 Wroclaw, Poland

autor

Dziegiel P.

autor

Sopel M.

autor

Wojnar A.

autor

Zabel M.

Bibliografia

• 1. Dziegiel P, Forgacz J, Suder E, Surowiak P, Kornafel J, Zabel M (2003) Prognostic significance of metallothionein expression in correlation with Ki-67 expression in adenocarcinomas of large intestine. Histol Histopathol, 18: 401–407.
• 2. Gryfe R, Swallow C, Bapat B, Redston M, Gallinger S, Couture J (1997) Molecular biology of colorectal cancer. Curr Probl Cancer, 21: 233–300.
• 3. Levadoux-Martin M, Hesketh JE, Eattie JH, Wallace HM (2001) Influence of metallothionein-1 localization on its function. Biochem J, 355: 473–479.
• 4. Mady HH, Melhem MF (2002) FHIT protein expression and its relation to apoptosis, tumor histologic grade and prognosis in colorectal adenocarcinoma: an immunohistochemical and image analysis study. Clin Exp Metastasis, 19: 351–358.
• 5. Nagel WW, Valee BL (1995) Cell cycle regulation of metallothionein in human colonic cancer cells. Proc Natl Acad Sci USA, 92: 579–583.
• 6. Remmele W, Stegner HE (1987) Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue. Pathologe, 8: 138–140.
• 7. Sinicrope FA, Hart J, Hsu HA, Lemoine M, Michelassi F, Stephens LC (1999) Apoptotic and mitotic indices predict survival rates in lymph node-negative colon carcinomas. Clin Cancer Res, 5: 1793–1804.
• 8. Wronkowski Z, Zwierko M (1996) Epidemiologia, czynniki ryzyka i możliwość zapobiegania. In: Nowacki MP (ed.). Nowotwory jelita grubego. Wydawnictwo Wiedza i Życie, Warszawa, pp. 24–50.