The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay [NMD] in plants

• Autorzy: Dzikiewicz-Krawczyk A. , Piontek P. , Szweykowska-Kulinska Z. , Jarmolowski A.
• Języki publikacji: EN

Abstrakty

EN

In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC–). For each gene the PTC+/PTC– ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC– splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC– ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.

Słowa kluczowe

PL

plant; nonsense-mediated mRNA decay; nuclear cap-binding protein complex; gene; mRNA variant; Arabidopsis thaliana; mutant; wild plant; mRNA surveillance

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 4
• Opis fizyczny: p.825-828,fig.,ref.

Twórcy

autor

Dzikiewicz-Krawczyk A.

• Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

autor

Piontek P.

autor

Szweykowska-Kulinska Z.

autor

Jarmolowski A.

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