Wheat anther culture as affected by various cultural changes and supplements

• Autorzy: Simonson R L , Baenziger P S , Gustafson V D
• Języki publikacji: EN

Abstrakty

EN

With the numerous improvements in cereal tissue and wheat anther culture, it is necessary to determine which of the improvements should be combined for optimal response. This study was conducted using one highly responsive cultivar of wheat (Triticum aestivum L. cv. Pavon 76) to test the effectiveness of pre-culture cold treatment (0 or 3-7 days at 5°C) of anthers, five initiation basal media, and various changes in Murashige-Skoog regeneration media. A cold pre-culture treatment was inhibitory for all initiation media for embryoid initiation. Of the initiation media, P1, 85D12, and N6 were similar for embryoid initiation (0.80 to 0.90 embryoids/anther) without a cold pre-culture treatment. Plant regeneration was improved by the addition of amino acids or glucose, increased sucrose concentration, filter sterilizing the medium, and altering plant growth regulator concentrations. P1 medium which is normally used for embryoid initiation was also beneficial for improving plant regeneration. Ethylene inhibitors were generally not beneficial.

Słowa kluczowe

EN

cultural change; supplement; wheat; silver nitrate; Triticum aestivum; anther culture; cereal tissue

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 4
• Opis fizyczny: p.381-392

Twórcy

autor

Simonson R L

• Terra International 600, Fourth St. Sioux City, Iowa, USA

autor

Baenziger P S

• Department of Agronomy, University of Nebraska, Lincoln, NE, USA

autor

Gustafson V D

• Department of Agronomy, University of Nebraska, Lincoln, NE, USA

Bibliografia

• Biddington N.L., Sutherland R.A., Robinson H.Т. (1988). Silver nitrate increased embryo production in anther culture of brussels sprouts. Annals Bot. 62: 181-185.
• Carmen J.G., Jefferson N.E., Campbell W.F. (1987a). Induction of embryogenic Triticum aestivum L. calli. I. Quantification of genotype and culture medium effects. Plant Cell Tissue Organ Cult. 10: 101-113.
• Carmen J.G., Jefferson N.E., Campbell W.F. (1987b). Induction of embryogenic Triticum aestivum L. calli. II. Quantification of organic addenda and other culture variable effects. Plant Cell Tissue Organ Cult. 10: 115-128.
• Chi G-L., Pua E-C. (1989). Ethylene inhibitors enhanced de novo shoot regeneration from cotyledons of Brassica campestris ssp. chinensis (chinese cabbage) in vitro. Plant Sci. 64: 243-250.
• Cho U-H., Kasha K.J. (1989). Ethylene production and embryogenesis from anther culture of barley (Hordeum vulgare). Plant Cell Rep. 8: 415-417.
• Сhu C.C. (1978). The N6 medium and its applications to anther culture of cereal crops. In: Proc. Symp. Plant Tissue Culture. Science Press, Beijing, China: 43-50.
• Сhu C.C., Hill R.D. (1988). An improved anther culture method for obtaining higher frequency of pollen embryoids in Triticum aestivum L. Plant Sci. 55: 175-181.
• Сhu C.C., Hill R.D., Brule-Babel A.L. (1990). High frequency of pollen embryoid formation and plant regeneration in Triticum aestivum L. on monosaccharide containing media. Plant Sci. 66: 255-262.
• Chuang C.C., Ouyang T.W., Chia H., Chan S.M., Ching C.K. (1978). A set of potato media for wheat anther culture. In: Proc. Symp. Plant Tissue Culture. Science Press, Beijing, China: 51-56.
• De-Buyser J., Henry Y. (1979). Androgenese sur des Bles tendres en cours selection. 1 - L’obtention des plantes in vitro. Z. Pflanzenzücht. 83: 49-56.
• Feng G.H., Ouyang J. (1988). The effects of KNO₃ concentration in callus induction medium for wheat anther culture. Plant Cell Tissue Organ Cult. 12: 3-12.
• Hassawi D.S., Qi J., Liang G.H. (1990). Effects of growth regulator and genotype on production of wheat and triticalc polyhaploids from anther culture. Plant Breeding 104: 40-45.
• Henry Y., De-Buyser J. (1981). Float culture of wheat anthers. Theor. Appl. Genet. 60: 77-79.
• Huang B. (1987). Effects of incubation temperature on microspore callus production and plant regeneration in wheat anther cultures. Plant Cell Tissue Organ Cult. 9: 45-48.
• Kelly S.L. (1986). The effect of sucrose, dextran and glucose on anther culture of winter wheat (Triticum aestivum L.). MSc Thesis, University of Guelph, Canada.
• Last D.I., Brettell R.I.S. (1990). Embryo yield in wheat anther culture is influenced by the choice of sugar in the culture medium. Plant Cell Rep. 9: 14-16.
• Lazar M.D., Schaeffer G.W., Baenziger P.S. (1990). The effect of interactions of culture environment with genotype on wheat (Triticum aestivum L.) anther culture response. Plant Cell Rep. 8: 525-529.
• Liang G.H., McHughen A. (1987). Novel approaches to wheat improvement. In: Wheat and Wheat Improvement (E.G. Heyne, ed.). ASA, CSSA, SSSA Publishers, Madison, Wis.: 472-506.
• Liang G.H., Sangduen N., Heyne E.G., Sears R.G. (1982). Polyhaploid production through anther culture in common wheat. J. Hered. 73: 360-364.
• Liang G.H., Xu A., Hoang T. (1987). Direct generation of wheat haploids via anther culture. Crop Sci. 27: 336-339.
• Marburger J.E., Sammons D.J., Schaeffer G.W. (1987). Effect of a modified potato medium on anther culture of wheat. Crop Sci. 27: 351-354.
• Marsolais A. A., Seguin-Swartz G., Kasha K.J. (1984). The influence of anther cold pretreatments and donor plant genotypes on in vitro androgenesis in wheat (Triticum aestivum L.). Plant Cell Tissue Organ Cult. 3: 69-79.
• Mathias R.J., Boyd L.A. (1986). Cefotaxime stimulates callus growth, embyrogenesis and regeneration in hexaploid bread wheat (Triticum aestivum L. em. Thell). Plant Sci. 46: 217-223.
• Murashige T., Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473-479.
• Olsen F.L. (1987). Induction of microsporc embryogenesis in cultured anthers of Hordeum vulgare L. The effect of ammonium nitrate, glutamine and asparagine as nitrogen sources. Carlsberg Res. Commun. 52: 393-404.
• Orshinsky B.R., McGregor L.J., Johnson G.I.E., Hucl P., Kartha K.K. (1990). Improved embryoid induction and green shoot regeneration from wheat anthers cultured in medium with maltose. Plant Cell Rep. 9: 365-369.
• Ouyang J. (1986). Induction of pollen plants in Triticum aestivum. In: Haploids of Higher Plants in Vitro (Han H., Hongyuan Y., eds.). Springer-Verlag, NY: 26-41.
• Papenfuss J.M., Carmen J.G. (1987). Enhanced regeneration from wheat callus cultures using dicamba and kinetin. Crop Sci. 27: 588-593.
• Perl A., Aviv D., Galun E. (1988). Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene. Plant Cell Rep. 7: 403-406.
• Picard E., De Buyser J. (1975). Nouveaux rcsultats concernant la culture d’antheres in vitro de Ble tendre (Triticum aestivum L.). Effets d’un choc thermique et de la position de l’anthere dans l’epi. C.R. Acad. Sci. Paris 281: 127-130.
• Purnhauser L., Medgyesy P., Czako M., Dix P.J., Marton L. (1987). Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumba ginifolia Viv. tissue cultures using ethylene inhibitor AgNO₃ Plant Cell Rep. 6: 1-4.
• Qureshi J.A., Kartha K.K., Abrams S.R., Steinhauer L. (1989). Modulation of somatic embryogenesis in early and late-stage embryos of wheat (Triticum aestivum L.) under the influence of (+)-abscisic acid and its analogs. Plant Cell Tissue Organ Cult. 18: 55-69.
• Research Group 301, Institute of Genetics, Academia Sinica (1976). A sharp increase of the frequency of pollen plant induction in wheat with potato medium. Acta. Genet. Sin. 3: 25-31.
• Reynolds T.C. (1987). A possible role for ethylene during IAA induced pollen embryogenesis in anther cultures of Solanum carolinense L. Amer. J. Bot. 74: 967-969.
• Schaeffer G.W., Lazar M.D., Baenziger P.S. (1984). Wheat. In: Handbook of Plant Cell Culture, Vol. 2: Crop Species (W.R. Sharp, D.A. Evans, P.V. Ammirato, Y. Yamada, eds.). Macmillan Publishing Co., NY: 108-136.
• Shimada T. (1981). Haploid plants regenerated from the pollen callus of wheat (Triticum aestivum L.). Jpn. J. Genet. 56: 581-588.
• Simonson R.L., Baenziger P.S. (1992). The effect of gelling agents on wheat anther and immature embryo culture. Plant Breeding 109: 211-217.
• Thorn E.C. (1988). Effect of melibiose and polyethylene glycol on anther culture response in barley. EUCARPIA Meeting, Elsinore, Denmark, September 1988.
• Vain P., Yean H., Flament P. (1989). Enhancement of production and regeneration of cmbryogenic type II callus in Zea mays L. by AgNO₃. Plant Cell Tissue Organ Cult. 18: 143-151.
• Wei Z.M. (1982). Pollen callus culture in Triticum aestivum L. Thcor. Appl. Genet. 63: 71-73.
• Yuan H.-M., Keppenne V.D., Baenziger P.S., Berke T., Liang G.H. (1990). Effect of genotype and medium on wheat (Triticum aestivum L.) anther culture. Plant Cell Tissue Organ Cult. 21: 253-258.
• Zhou H., Konzak C.F. (1989). Improvement of anther culture methods for haploid production in wheat. Crop Sci. 29: 817-821.
• Zhou H., Zheng Y., Konzak C.F. (1991). Osmotic potential of media affecting green plant percentage in wheat anther culture. Plant Cell Rep. 10: 63-66.
• Zhu M., Xu A., Yuan M., Huang C., Yu Z., Wang L., Yu J. (1990). Effects of amino acids on callus differentiation in barley anther culture. Plant Cell Tissue Organ Cult. 22: 201-204.