Wplyw DDT i jego metabolitow na czynnosc sekrecyjna komorek lutealnych, granulozy i endometrium krowy

• Autorzy: Wrobel M , Mlynarczuk J. , Kotwica J.

Warianty tytułu

EN

Influence of DDT and its metabolites on the secretory function of luteal, granulosa and endometrial cells in cows

• Języki publikacji: PL

Abstrakty

EN

Dichlorodiphenyltrichloroethane (DDT) is a persistent insecticide, recognized as an environmental pollutant. Due to its lipophilic properties, DDT and its metabolite (DDE) are accumulated in tissues of farm animals. The aim of the study was to examine the influence of DDT and DDE on estradiol, progesterone and oxytocin secretion from the ovary and on prostaglandin (F2α and E2) secretion from the uterus, was investigated. Granulosa, luteal and endometrial cells from cows at 8-12 days of the estrous cycle were treated for 24-72 h with 0.1-10 ng/ml of DDT, p,p’-DDE, o,p’-DDE or with a technical mixture of DDE isomers. Neither DDT nor DDE were found to affect the viability of cells compared to the control. They also did not affect the secretion of estradiol from granulosa cells. The utilized pollutants increased (P < 0.05-0.001) the progesterone and oxytocin secretion from luteal and granulosa cells. They also stimulated (P < 0.05) PGF2á secretion but simultaneously reduced (P < 0.001) PGE2 secretion from endometrial cells. Hence the ratio of PGF2α to PGE2 was markedly changed, from 1:1 in the control, up to 1:4-10 in treated cells. It has been concluded that DDT and its metabolites may impair regulation of the estrous cycle in cows by stimulation of oxytocin secretion from luteal and granulosa cells and by stimulation of PGF2α and the simultaneous inhibition of PGE2 secretion from endometrial cells.

Słowa kluczowe

PL

komorki lutealne; bydlo; komorki blony sluzowej macicy; komorki ziarniste; krowy; macica; DDT; aktywnosc sekrecyjna; jajniki

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 04B
• Opis fizyczny: s.583-587,rys.,tab.,bibliogr.

Twórcy

autor

Wrobel M

• Instytut Rozrodu Zwierzat i Badan Zywnosci PAN, ul.Tuwima 10, 10-747 Olsztyn

autor

Mlynarczuk J.

autor

Kotwica J.

Bibliografia

• 1. Andric S. A., Kostic T. S., Stanko S. S., Kovacevic R. Z.: Inhibition of rat testicular androgenesis by a polychlorinated biphenyl mixture Aroclor 1248. Biol. Reprod. 2000, 62, 1882-1888.
• 2. Flint A. P. F., Sheldrick E. L., McCann T. J., Jones D. S. C.: Luteal oxytocin: characteristic and control of synchronous epizodes of oxytocin and PGF2α secretion at luteolysis in ruminats. Domest. Anim. Endocrinol. 1990, 7, 111-124.
• 3. Furuya K., Mizumoto Y., Makimura N., Mitsui C., Murakami M., Tokuoka S., Ishikawa N., Nagata I., Kimura T., Ivell R.: A novel biological aspect of ovarian oxytocin: gene expression of oxytocin, and oxytocin receptor in cumulus/luteal cells and the effect of oxytocin on embryogenesis in fertilized oocytes. Adv. Exp. Med. Biol. 1995, 395, 523-528.
• 4. Harper M. J. K.: Gamete and zygote transport, [w:] Knobil E., Neill J. (wyd.): The Physiology of Reproduction. Raven Press, New York 1988, 103-134.
• 5. Ireland J. J., Murphee R., Coulson P. B.: Accuracy of predicting stages of bovine estrous cycle by gross appearance of the corpus luteum. J. Dairy Sci. 1980, 63, 155-160.
• 6. Janošek J., Hilscherowá K., Bláha L., Holoubek I.: Environmental xenobiotics and nuclear receptors - interactions, effects and in vitro assessment. Toxicol. in Vitro 2006, 20, 18-37.
• 7. Kamarianos A., Karamanlis X., Goulas P., Theodosiadou E., Smokovitis A.: The presence of environmental pollutants in follicular fluid of farm animals (cattle, sheep, goats and pigs). Reprod. Toxicol. 2003, 17, 185-190.
• 8. Kuiper G. G. J. M., Lemmen J. G., Carlsson B., Corton J. C., Safe S. H., van der Saag P. T., van der Burg B., Gustafsson J. A.: Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor. Endocrinology 1998, 139, 4252-4263.
• 9. Longnecker M. P., Klebanoff M. A., Dunson D. B., Guo X., Chen Z., Zhou H., Brock J. W.: Maternal serum level of the DDT metabolite DDE in relation to fetal loss in previous pregnancies. Environ. Res. 2005, 97, 127-133.
• 10. Luck M. R.: Enhanced secretion of oxytocin from bovine granulosa cells treated with adrenal steroids. J. Reprod. Fertil. 1988, 83, 901-907.
• 11. Lundholm C. E.: DDE-induced eggshell thinning in birds: effects of p,p'-DDE on the calcium and prostaglandin metabolism of the eggshell gland. Comp. Biol. Physiol. 1997, 118, 113-128.
• 12. McCracken J. A., Schramm W., Okulicz W.: Hormone receptor control of pulsatile secretion of PGF2α from the ovine uterus during luteolysis and its abrogation in early pregnancy. Anim. Reprod. Sci. 1984, 7, 31-55.
• 13. Miyamoto A., Schams D.: Oxytocin stimulates progesterone release from microdialyzed bovine corpus luteum in vitro. Biol. Reprod. 1991, 44, 1163-1170.
• 14. Młynarczuk J., Kotwica J.: Effect of polychlorinated biphenyls on the secretion of oxytocin from luteal and granulosa cells in cow: possible involvement of glucocorticoid receptors. Vet. Med.-Czech 2006, 51, 391-398.
• 15. Młynarczuk J., Kotwica J.: Influence of polychlorinated biphenyls on LH-stimulated secretion of progesterone and oxytocin from bovine luteal cells. Pol. J. Vet. Sci. 2006, 9, 101-108.
• 16. Młynarczuk J., Niewiadomska A., Kotwica J.: Polychlorinated biphenyls impair FSH-stimulated effect on the secretion of steroids and oxytocin from bovine granulosa cells. Bull. Vet. Inst. Pulawy 2005, 49, 411-417.
• 17. Nejaty H., Lacey M., Whitehead S. A.: Differing effects of endocrine-disrupting chemicals on basal and FSH-stimulated progesterone production in rat granulosa-luteal cells. Exp. Biol. Med. 2001, 226, 570-576.
• 18. Nikula H., Talonpoika T., Kaleva M., Toppari J.: Inhibition of hCG stimulated steroidogenesis in cultured mouse Leydig tumor cells by bisphenol A and octylphenols. Biochem. Pharmacol. 1999, 157, 166-173.
• 19. Rogan W. J., Chen A.: Health fisks and benefits of bis(4-chlophenyl)-1,1,1-trichloroethane (DDT). Lancet 2005, 366, 763-773.
• 20. Silvia W. J., Lewis G. S., McCracken J. A., Thatcher W. W., Wilson L. Jr.: Hormonal regulation of uterine secretion of prostaglandin F2α during luteolysis in ruminants. Biol. Reprod. 1991, 45, 655-663.
• 21. Stormshak F.: Biochemical and endocrine aspects of oxytocin production by the mammalian corpus luteum. Reprod. Biol. Endocrinol. 2003, 1, 92.
• 22. Tiemann U., Pöhland R., Schneider F.: Influence of organochlorine pesticides on physiological potency of cultured granulosa cells from bovine preovulatory follicles. Theriogenology 1996, 46, 253-265.
• 23. Trigg P. I., Kondrachine A. V.: Commentary: malaria control in the 1990s. Bull. World Health Organ. 1998, 76, 11-16.
• 24. Voss A. K., Fortune J. E.: Estradiol-17β has biphasic effect on oxytocin secretion by bovine granulosa cells. Biol. Reprod. 1993, 48, 1404-1409.
• 25. Wójtowicz A. K., Gregoraszczuk E. L., Ptak A., Falandysz J.: Effect of single and repeated in vitro exposure of ovarian follicles to o,p'-DDT and p,p' -DDT and their metabolites. Pol. J. Pharmacol. 2004, 56, 465-472.
• 26. Wójtowicz A. K., Milewicz T., Gregoraszczuk E. L.: DDT and its metabolite DDE alter steroid hormone secretion in human term placental explants by regulation of aromatase activity. Toxicol. Lett. 2007, 173, 24-30.
• 27. Wróbel M., Kamiński K., Kotwica J.: In vitro effects of polychlorinated biphenyls (PCBs) on the contractility of bovine myometrium from the periovulatory stage of the estrous cycle. Reprod. Biol. 2005, 5, 303-319.
• 28. Wróbel M., Kotwica J.: Influence of polychlorinated biphenyls (PCBs) and phytoestrogens on prostaglandin F2á and E2 secretion from bovine endometrial cells at a postovulatory stage of the oestrous cycle. Vet. Med.-Czech 2005, 50, 487-495.
• 29. Zava D. T., Blen M., Duwe G.: Estrogenic activity of natural and synthetic estrogens in human breast cancer cells in culture. Environ. Health. Perspect. 1997, 105, 637-645.