Superoxide dismutase activity in organs of Mesembryanthemum crystallinum L. at different stages of CAM development

• Autorzy: Libik M , Konieczny R. , Surowka E. , Miszalski Z.
• Języki publikacji: EN

Abstrakty

EN

Fluctuations of acidity were found in leaves, shoots and roots of two groups of Mesembryanthemum crystallinum plants: watered and salt-treated. This suggests that the functioning of CAM (related to malate accumulation) in the tested organs can differ in "early CAM" in watered plants versus "full CAM" in NaCl-irrigated plants. Among the different organs analyzed, shoots exhibited the highest acidity level at the beginning of the day in both groups of M. crystallinum plants. Superoxide dismutase forms (MnSOD, CuZnSOD and FeSOD) differed in activity depending not only on their localization in the plant tissue but also on the stage of CAM development. Plants exhibiting "full CAM" and showing clear diurnal ∆-acidity had much higher total SOD activity during evening hours than in plants following "early CAM" photosynthesis. Shoots showed the highest activity of SOD.

Słowa kluczowe

EN

superoxide dismutase; plant physiology; organ; oxidative stress; Mesembryanthemum crystallinum; salinity; leaf; shoot; root

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2005
• Tom: 47
• Numer: 1
• Opis fizyczny: p.199-204,fig.,ref.

Twórcy

autor

Libik M

• Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland

autor

Konieczny R.

autor

Surowka E.

autor

Miszalski Z.

Bibliografia

• Alscher RG, Ertuk N, and Health LS. 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany 53: 1331-1341.
• Adams P, Nelson DE, Yamada S, Chmara W, Jensen RG, Bohnert HJ, and Griffiths H. 1998. Growth and development of Mesembryanthemum crystallinum (Aizoaceae). New Phytologist 138: 171-190.
• Beauchamp C, and Fridovich I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44: 276-287.
• Bowler C, Montagu M, and Inzé D. 1992. Superoxide dismutase and stress tolerance. Annual Review of Plant Physiology and Plant Molecular Biology 43: 83-116.
• Bradford MM. 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
• Broetto F, Lüttge U, and Ratajczak R. 2002. Influence of light intensity and salt-treatment on the mode of photosynthesis and enzymes of the antioxidant response system of Mesembryanthemum crystallinum. Functional Plant Biology 29: 13-23.
• Corpas FJ, Gomez M, Hernández JA, and Del Rio LA. 1993. Metabolism of activated oxygen in peroxisomes from two Pisum sativum L. cultivars with different sensitivity to sodium chloride. Journal of Plant Physiology 141: 160-165.
• Cheng S-H, and Edwards GE. 1991. Induction of Crassulacean acid metabolism in the facultative halophyte Mesembryanthemum crystallinum. Plant Cell and Environment: 14: 271-278.
• Chu C, Dai Z, Ku MSB, and Edwards G. 1990. Induction of Crassulacean acid metabolism in the facultative halophyte Mesembryanthemum crystallinum by abscisic acid. Plant Physiology 93: 1253-1260.
• Cushman JC, and Bohnert HJ. 1997. Molecular genetics of Crassulacean acid metabolism. Plant Physiology 113: 667-676.
• Cushman JC, Michalowski CHB, and Bohnert HJ. 1990. Developmental control of Crassulacean acid metabolism inducibility by salt stress in common ice plant. Plant Physiology 94: 1137-1142.
• Dat J, Vandenabeele S, Vranová E, van Montagnu M, Inzé D, and Breusegem F. 2000. Dual action of the active oxygen species during plant cell responses. Cell Molecular Life Science 57: 779-795.
• del Río LA, Lyon DS, Olah I, Glick, and Salín ML. 1983. Immunocytochemical evidence for a peroxisomal localization of manganese superoxide dismutase in leaf protoplasts from higher plant. Planta 158: 216-224.
• Eastmond PJ, and Ross JD. 1997. Evidence that the induction of crassulacean acid metabolism by water stress in Mesembryanthemum crystallinum (L.) involves root signaling. Plant Cell and Environment 20: 1559-1565.
• Fadzilla NM, Finch RP, and Burdon RH. 1997. Salinity, oxidative stress and antioxidant responses in shoot cultures of rice. Journal of Experimental Botany 48: 325-331.
• Foster JG, and Edwards GE. 1980. Localization of superoxide dismutase in leaves of C3 and C4 plants. Plant Physiology 107: 757-750.
• Hernández JA, Olmos E, Corpas FJ, Sevilla F, and del Río LA. 1995. Salt-induced oxidative stress in chloroplasts of pea plants. Plant Science 105: 151-167.
• Hernández JA, Campillo A, Jimenez A, Alarcon JJ, and Sevilla F. 1999. Response of antioxidant systems and leaf water relations to NaCl stress in pea plants. New Phytologist 141: 241-251.
• Hibberd J, and Quick WP. 2002. Characteristics of C4 photosynthesis in stem and petioles of C3 flowering plants. Nature 415: 451-454.
• Laemmli UK. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685 .
• Libik M, Pater B, Elliot S, Ślesak I, and Miszalski Z. 2004. Malate accumulation in different organs of Mesembryanthemum crystallinum L. following age-dependent or salinity triggered CAM metabolism. Zeitschrift für Naturforschung 59c: 223-228.
• Libik M, Konieczny R, Pater B, Ślesak I, and Miszalski Z. 2005. Differences in the activities of some antioxidant enzymes and in H2O2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant. Plant Cell Reports 23: 834-841.
• Lüttge U. 1993. The role of crassulacean acid metabolism (CAM) in adaptation of plants to salinity. New Phytologist 125: 59-71.
• Lüttge U. 2002. Performance of plants with C4-carboxylation modes of photosynthesis under salinity. In: Lauchli A, Lüttge U [eds.]. Salinity: environment-plants-molecules, 113-135. Kluwer, Dordrecht.
• Lüttge U. 2004. Ecophysiology of Crassulacean acid metabolism (CAM). Annals of Botany 93: 629-652.
• McCord JM, and Fridovich I. 1969. Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry 244: 6049-6055.
• Miszalski Z, Ślesak I, Niewiadomska E, Baczek-Kwinta R, Lüttge U, and Ratajczak R. 1998. Subcellular localization and stress responses of superoxide dismutase isoforms from leaves in the C3-CAM intermediate halophyte Mesembryanthemum crystallinum L. Plant, Cell and Environment 21: 169-179.
• Niewiadomska E, Miszalski Z, and Pilipowicz M. 1997. The presence of FeSOD in the Aceraceae family. Phyton 37: 215-218.
• Olmos E, Hernández JA, Sevilla F, and Hellin E. 1994. Induction of several antioxidant enzymes in the selection of a salt-tolerant cell line of Pisum sativum. Journal of Plant Physiology 144: 594-598.
• Sakamoto A, Okumura T, Maninak H, and Tanaka K. 1995. Molecular cloning of the gene (SodCc1) that encodes a cytosolic copper zinc-superoxide dismutase from rice (Oryza sativa L.). Plant Physiology 107: 651-652.
• Salin ML, and Bridges SM. 1980. Isolation and characterization of an iron-containing superoxide dismutase from an Eucaryote Brassica campestris. Archives of Biochemistry and Biophysics 201: 369-374.
• Salin ML, and Bridges SM. 1981. Localization of superoxide dismutase in chloroplasts from Brassica campestris. Zeitschrift für Pflanzenphysiologie 99: 37-45.
• Scandalios JG. 1993. Oxygen stress and superoxide dismutases. Plant Physiology 101: 7-12.
• Streller S, Kromer S, and Wingsle G. 1994. Isolation and purification of mitochondrial Mn-superoxide dismutase from Gymnosperm Pinus sylvestris L. Plant Cell Physiology 35: 859-867.
• Ślesak I, Karpińska B, Surówka E, Miszalski Z, and Karpiński S. 2003. Redox changes in the chloroplasts and hydrogen peroxide are essential for regulation of C3-CAM transition and photooxidative stress responses in the facultative CAM plant Mesembryanthemum crystallinum. Plant Cell Physiology 44: 573-581.
• Ślesak I, and Miszalski Z. 2003. Superoxide dismutase-like protein from roots of the intermediate C3-CAM plant Mesembryanthemum crystallinum L. in in vitro culture. Plant Science 164: 497-505.
• Winter K. 1973. Zum Problem der Ausbildung des Carssulaceaen - Säurestoffwechsels bei Mesembryanthemum crystallinum unter NaCl-Einfluß. Planta 109: 135-145.
• Winter K, Lüttge U, Winter E, and Troughton JH. 1978. Seasonal shift from C3 photosynthesis to Crassulacean acid metabolism in Mesembryanthemum crystallinum growing in its natural environment. Oecologia 34: 225-237.