Experimental Chagas' disease: The protection induced by immunization with Trypanosoma rangeli is associated with down-regulation of IL-6, TNF-alpha and IL-10 synthesis

• Autorzy: Cervetta L , Moretti E , Basso B
• Języki publikacji: EN

Abstrakty

EN

In an experimental model developed in our laboratory, BALB/c mice were protected against Trypanosoma cruzi infection by immunization with T. rangeli, a parasite closely related to T. cruzi but not pathogenic for man. Immunized mice developed low parasitemia and high survival rates when challenged with T. cruzi. In the present work, we study the patterns of systemic production of interleukin (IL-6), tumor necrosis factor alfa (TNF-α), interferon gamma (IFN-γ), IL-2, IL-4 and IL-10 during the acute phase of T. cruzi infection in mice previously immunized with T. rangeli with respect to only infected mice. The results showed a concentration of IL-6, TNF-α and IL-10 significantly lower in immunized mice than in only infected ones. The production of IFN-γ, a cytokine involved in resistance, was preserved in vaccinated mice. Finally, both groups of animals showed IL-2 and IL-4 serum levels similar to those observed in uninfected mice. As in other experimental or human infections, in this model, the pattern of systemic production of cytokines can be associated with the outcome of infection.

Słowa kluczowe

EN

infection; mice; Chagas disease; interleukin 10; Trypanosoma rangeli; parasite; interleukin 6; mouse; tumour necrosis factor-alpha; cytokine; protection; immunization; synthesis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2002
• Tom: 47
• Numer: 1
• Opis fizyczny: p.73-78,fig.

Twórcy

autor

Cervetta L

• Servicio Nacional de Chagas & Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina

autor

Moretti E

• Servicio Nacional de Chagas & Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina

autor

Basso B

• Servicio Nacional de Chagas & Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina

Bibliografia

• Abrahamsohn I., Coffman R. 1996. Trypanosoma cruzi: IL-10, TNF, IFN-gamma, and IL-12 regulate innate and acquired immunity to infection. Experimental Parasitology, 84, 231-244.
• Barbosa de Oliveira L., Curotto de Lafaille M., Collet de Araujo Lima G., Abrahamsohn I. 1996. Antigen-specific IL-4 and IL-10 secreting CD4+ lymphocytes increase in vivo susceptibility to Trypanosoma cruzi infection. Cellular Immunology, 170, 41-53.
• Basso B., Moretti E., Rodriguez M. 1980. Cultivo de Trypanosoma cruzi en medio monofásico con aireación para la obtención de antígenos. Medicina (Bs. As.), 40, 428-432.
• Basso B., Moretti E., Vottero-Cima E. 1987. Antigenic relationships between Trypanosoma cruzi and Trypanosoma rangeli. Revista Ibérica de Parasitología, 47, 15-21.
• Basso B., Moretti E., Vottero-Cima E. 1991. Immune response and Trypanosoma cruzi infection in Trypanosoma rangeli immunized mice. American Journal of Tropical Medicine and Hygiene, 44, 413-419.
• Cardillo F., Voltarelli J.C., Reed S.G., Silva J.S. 1996. Regulation of Trypanosoma cruzi infection in mice by gamma interferon and interleukin 10: role of NK cells. Infection and Immunity, 64, 128-134.
• Cunningham D., Khun R. 1980. T. cruzi induced suppression of the primary immune response in murine cell cultures to T cell- dependent and independent antigens. Journal of Parasitology, 66, 16-27.
• Chandrasekar B., Melby P.C., Troyer D.A., Freeman G.L. 1996. Induction of proinflammatory cytokine expression in experimental acute chagasic cardiomyopathy. Biochemical and Biophysical Research Communication, 223, 365-371.
• Chandrasekar B., Melby P.C., Troyer D.A., Colston J.T., Freeman G.L. 1998. Temporal expression of pro-inflammatory cytokines and inducible nitric oxide synthase in experimental acute chagasic cardiomyopathy. American Journal of Pathology, 152, 925-934.
• Fresno M., Kopf M., Rivas L. 1997. Cytokines and infectious diseases. Immunology Today, 18, 56-58.
• Gazzinelli R.T., Oswald I.P., Hieny S., James S.L., Sher A. 1992. The microbicidal activity of interferon-γ-treated macrophages against Trypanosoma cruzi involves an L-arginine-dependent, nitrogen oxide-mediated mechanism inhibitable by interleukin-10 and transforming growth factor-ß. European Journal of Immunology, 22, 2501-2506.
• Gomez L., Nasser J., Basombrio M. 1996. Complete immunization against Trypanosoma cruzi verified in individual mice by complement mediated lysis. Memorias do Instituto Oswaldo Cruz, 91, 56-61.
• Harel-Bellan A., Joskowicz M., Fradelizi D., Bisen H. 1983. Modification of T-cell proliferation and interleukin 2 production in mice infected with Trypanosoma cruzi. Proceedings of the National Academy of Sciences, USA, 80, 3466-3469.
• Hoft D., Lynch R., Kicchoff L. 1993. Kinetic analysis of antigen-specific immune responses in resistant and susceptible mice during infection with Trypanosoma cruzi. Journal of Immunology, 151, 7038-7047.
• Introini M., Basso B., Moretti E. 1998. Enfermedad de Chagas experimental: I estudio de diferentes condiciones de immunización sobre el curso de la infección. Boletín Chileno de Parasitología, 53, 45-51.
• Kierszenbaum F., Moretti E., Sztein M. 1993. Molecular basis of T. cruzi induced immunosuppression. Altered expression by activated human lymphocytes of molecules which regulate antigen recognition and progression through the cell cycle. Biological Research, 26, 197-207.
• Locksley R.M., Scott P. 1991. Helper T-cell subset in mouse leishmaniasis: induction, expansion and effector function. Immunology Today, 12, A58-A61.
• Miller M., Wrightsman R., Manning J. 1996. Trypanosoma cruzi: protective immunity in mice immunized with paraflagellar rod proteins is associated with a T-helper type 1 response. Experimental Parasitology, 84, 156-167.
• Nabors G., Tarleton R. 1991. Differential control of IFN-gamma and IL-2 production during Trypanosoma cruzi infection. Journal of Immunology, 146, 3591-3598.
• Petray P., Castaños-Velez E., Grinstein S., Orn A., Rottenberg M. 1995. Role of nitric oxide in resistance and histopathology during experimental infection with Trypanosoma cruzi. Immunology Letters, 47, 121-126.
• Reed S. 1988. In vivo administration of recombinant interferon gamma induces macrophage activation, and prevents acute disease, immune suppression, and death in experimental Trypanosoma cruzi infections. Journal of Immunology, 140, 4342-4347.
• Reed S., Brownell C., Russo D., Silva J., Grabstein K., Morrisey P. 1994. IL-10 mediates susceptibility to Trypanosoma cruzi infection. Journal of Immunology, 153, 3135-3140.
• Revelli S., Didoli G., Roggero E., Moreno H., Bernabo J., Wietzerbin J., Bottasso O. 1998. Macrophage activity, IL-6 levels, antibody response and heart histology in rats undergoing an attenuated Typanosoma cruzi acute infection upon treatment with recombinant interferon gamma. Cytokines Cellular and Molecular Therapy, 4, 153-159.
• Revelli S., Gomez L., Wietzerbin J., Bottasso O., Basombrio M.A. 1999. Levels of tumor necrosis factor alpha, gamma interferon, and interleukins 4, 6, and 10 as determined in mice infected with virulent or attenuated strains of Trypanosoma cruzi. Parasitology Research, 85, 147-150.
• Rivera M., Marques de Araujo S., Lucas R., Deman J., Truyens C., Defresno M., Baetselier P., Carlier Y. 1995. High tumor necrosis factor alpha (TNF-α) production in Trypanosoma cruzi infected pregnant mice and increased TNF-α gene transcription in their offspring. Infection and Immunity, 63, 591-595.
• Saldana A., Sousa O. 1996. Trypanosoma rangeli: epimastigote immunogenicity and cross-reaction with Trypanosoma cruzi. Journal of Parasitology, 82, 363-366.
• Saldana A., Sousa O., Orn A. 1995. Immunoparasitological studies of Trypanosoma cruzi low virulence clones from Panama: humoral immune responses and antigenic cross-reactions with Trypanosoma rangeli in experimental infected mice. Scandinavian Journal of Immunology, 42, 644-650.
• Silva J.S., Morrisey P.J., Grabstein K.H., Mohler K.M., Anderson D., Reed S.G. 1992. Interleukin 10 and interferon gamma regulation of experimental Trypanosoma cruzi infection. Journal of Experimental Medicine, 175, 169-174.
• Silva J., Vespa G., Cardoso M., Aliberti J., Cunha F. 1995. Tumor necrosis factor alpha mediates resistance to Trypanosoma cruzi infection in mice by inducing nitric oxide production in infected gamma interferon activated macrophages. Infection and Immunity, 63, 4862-4867.
• Tanowitz H., Grumprecht J., Spurr D., Calderon T., Ventura M., Raventos-Suarez C., Kellie S., Factor S., Hatcher V., Wittner M. 1992. Cytokine gene expression of endothelial cells infected with Trypanosoma cruzi. Journal of Infectious Diseases, 166, 598-603.
• Tarleton R. 1997. Immunity to Trypanosoma cruzi. In: Host response to intracellular pathogens (Ed. H.E. Stefan). R. G. Landers Company.
• Torrico F., Heremans H., Rivera M.T., Van Marck E., Billiau A., Carlier Y. 1991. Endogenous IFN-γ is required for resistance to acute Trypanosoma cruzi infection in mice. Journal of Immunology, 146, 3626-3632.
• Truyens C., Torrico F., Angelo-Barrios A., Lucas R., Heremans H., De Baetselier P., Carlier Y. 1995. The cachexia associated with Trypanosoma cruzi acute infection in mice is attenuated by anti-TNF-α, but not by anti-IL-6 or anti-IFN-γ antibodies. Parasite Immunology, 17, 561-568.
• Wrightsman R., Miller J., Saborio J., Manning J. 1995. Pure paraflagellar rod protein protects mice against Trypanosoma cruzi infection. Infection and Immunity, 63, 122-127.
• Zhang L., Tarleton R.L. 1996. Characterization of cytokine production in murine Trypanosoma cruzi infection by in situ immunocytochemistry: lack of association between susceptibility and type 2 cytokine production. European Journal of Immunology, 26, 102-109.
• Zuniga C., Palau T., Penin P., Gamallo C., de Diego J. 1997. Protective effect of Trypanosoma rangeli against infections with a highly virulent strain of Trypanosoma cruzi. Tropical Medicine and International Health, 2, 482-487.