Histological estimation of the small intestine wall after administration of feed containing deoxynivalenol, T-2 toxin and zearalenone in the pig

• Autorzy: Obremski K. , Zielonka L. , Gajecka M. , Jakimiuk E. , Bakula T. , Baranowski M. , Gajecki M.
• Języki publikacji: EN

Abstrakty

EN

Fusarium spp. moulds are common in moderate climate regions of North America, Asia and Europe. They produce hepatotoxic and nephrotoxic mycotoxins, acting like estrogens, impairing heniopoesis and immunosuppressing. Actively dividing skin cells, lymphatic tissue, haemopoetic tissue and gastrointestinal tissue are the most sensitive for these trichothecenes action. The mucosal membrane of the gastrointestinal tract is the first barrier of the organism contacting with foreign antigens like feed proteins, natural toxins, saprophytic and pathogenic microflora and mycotoxins. The aim of this study was to perform histological estimation of the porcine small intestine after short term intoxication with low doses of deoxynivalenol (DON), T-2 toxin (T-2) and zearalenone (ZEA) obtained from wheat naturally contaminated with Fusarium moulds. Experimental pigs (n=5) were fed for 14 days feed containing DON, T-2 and ZEA (28.9, 11.5 and 33.2 μg kg-1 of feed). On the last day of the experiment, the animals were euthanised and samples of the jejunum were collected for histological examination. In the experimental pigs, normally developed intestinal villi and crypts were found. However, number of acidophilic granulocytes in the mucous membrane and decreased numbers of goblet cells, increased numbers of endothelial lymphocytes and numerous plasma cells in intestinal epithelium was observed. On the surface of the intestinal epithelium the glycocalyx was poorly developed. The results obtained suggest that short term intoxication with low doses of DON, T-2 and ZEA does not cause significant changes in the histological structure of the small intestine in the pig. However, low concentrations of DON. T-2 and ZEA probably influence enterocytes metabolism and evoke inflammation of the mucous membrane of the small intestine.

Słowa kluczowe

EN

pig; Fusarium; mycotoxin; immunosuppression; gastrointestinal tract; small intestinal wall; feed; deoxynivalenol; T-2 toxin; zearalenone; histology; intestine; animal feeding

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2008
• Tom: 11
• Numer: 4
• Opis fizyczny: p.339-345,fig.,ref.

Twórcy

autor

Obremski K.

• University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Zielonka L.

autor

Gajecka M.

autor

Jakimiuk E.

autor

Bakula T.

autor

Baranowski M.

autor

Gajecki M.

Bibliografia

• Biehl ML, Prelusky DB, Koritz GD, Hartin KE, Buck WB, Trenholm HL (1993) Billiary excretion and en- terohepatic cycling of zearalenone in immature pigs. Toxicol Appl Pharmacol 121: 152-159.
• Bouhet S, Oswald IP (2005) The effects of mycotoxins, fun­gal food contaminants, on the intestinal epithelial cell-derived innate immune response. Vet Immunol Im- munopathol 108: 199-209.
• Brown T, Rottinghaus G, Williams M (1992) Fumonisin mycotoxicosis in broilers: performances and pathology. Avian Dis 36: 450-454.
• Bulerman LB (1996) Occurrence of fusarium and fumonisins on food grains and in foods. Adv Exp Med Biol 392: 27-38.
• Cavret S, Lecoeur S (2006) Fusariotoxin transfer in animal. Food Chem Toxicol 44: 444-453.
• Curtui VG, Gareis M, Usleber E, Martlabauer E (2001) Survey of Romanian slaughtered pigs for the occurrence of mycotoxins ochratoxins A and B, and zearalenone. Food Addit Contam 18: 730-738.
• Howard MD, Gordon DT, Pace LW, Garleb KA, Kerley MS (1995) Effects of dietary supplementation with fruc- tooligosacchachides on colonic microbiota populations and epithelial cell proliferation in neonatal pigs. J Pediatr Gastroenterol Nutr 21: 297-303.
• Gajęcki M, Gajęcka M, Zielonka Ł, Jakimiuk E, Obremski K (2006) Zearalenone as a potential allergen in the alimentary tract - a review. Pol J Food Nutr Sci 15/56: 263-268.
• Jiang R, Chang X, Stoll B, Fan MZ, Arthington J, Weaver E, Campbell J, Burrin DG (2000) Dietary plasma pro­tein reduces small intestinal growth and lamina propria cell density in early weaned pigs. J Nutr 130: 21-26.
• Li G, Shinozuka J, Uetuka K, Nakayama H, Doi K (1997) T-2 toxin-induced apoptosis in intestinal crypts epi­thelial cells of mice. Exp Toxicol Pathol 49: 447-450.
• Montagne L, Piel C, Lalles JP (2004) Effect of diet on mucin kinetics and composition: nutrition and health implications. Nutr Rev 62: 105-114.
• Morita Y, Sawada M, Seno H, Takaishi S, Fukuzawa H, Miyake N, Hiai H, Chiba T (2001) Identification of xan­thine dehydrogenase/xanthine oxidase as a rat Paneth cell zinc-binding protein. Biochim Biophys Acta 1540: 43-49.
• Obremski K, Gajęcka M, Zielonka Ł, Jakimiuk E, Gajęcki M (2005) Morphology and ultrastructure of small intes­tine mucosa in gilts with zearalenone mycotoxicosis. Pol J Vet Sci 8: 301-307.
• Perkowski J, Miedaner T, Geiger HH, Muller NM, Chelkowski J (1995) Occurrence of deoxynivalenol (DON). 3-Acetyl-DON, Zearalenone, and Ergosterol in Winter Rye Inoculated with Fusarium culmorum. Cereal Chem 72:205-209.
• Pestka JJ (2003) Deoxynivalenol-induced IgA production and IgA nephropaty-aberrant mucosal immune re­sponse with systemic repercussion. Toxicol Lett 140-141: 287-295.
• Romański K (2007) Podstawy immunologii przewodu pokarmowego. Med Weter 63: 768-772.
• Tornwall J, Carem AB, Fox RI (1999) Estrogen in autoim­munity: expression of estrogen receptors in thymic and autoimmune T cells. J Gend Specif Med 2: 33-40.