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2005 | 54 | 1 |
Tytuł artykułu

The presence of free shiga-like toxins in stool specimens of patients with diarrhea during one year study

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
During one year study, 394 stool samples obtained from a random cases of diarrhea were examined for the presence of free shiga-like toxins Stx1 and Stx2 in Vero cells assay. Of 394 stool specimen supernatants tested, 2 (0.5%) gave positive results. The two stool supernatants positive on Vero cell line were wholly capable of being neutralized with the monoclonal antibody to Stx2. Broth cultures of strains isolated from the two positive stool samples were negative for Stxs-production in Vero cytotoxicity assay and by PCR.
Wydawca
-
Rocznik
Tom
54
Numer
1
Opis fizyczny
p.69-71,fig.,ref.
Twórcy
  • University of Medicine, 4 Chalubinskiego Street, 50-368 Wroclaw, Poland
autor
autor
Bibliografia
  • Boyce T.G., A.G. Pemberton, J.G. Wells and RM Griffin. 1995. Screening for Escherichia coli Ol 57:H7- a nationwide survey of clinical laboratories. J. Clin. Microbiol. 33: 3275-3277.
  • Karch H., T. Meyer, H. Rüssmann and J. Heesemann. 1992. Frequent loss of shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation. Infect. Immun. 60: 3464-3467.
  • Karch H., H. Rüssmann, H. Schmidt, A. Scharzkopf and J. Heesemann. 1995. Long-term shedding and clonal turnover of enterohemorrhagic Escherichia coli 0157 in diarrheal diseases. J. Clin. Microbiol. 33: 1602-1605.
  • Karmali M.A., M. Petric, C. Lim, R. Cheung and G.S. Arbus. 1985. Sensitive method for detecting low numbers of verotoxin-producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin. J. Clin. Microbiol. 22: 614-619.
  • Krishnan C, V.A. Fitzgerald, S.J. Dakin and R.J. Behme. 1987. Laboratory investigation of outbreak colitis caused by Escherichia coli 0157: H7. J. Clin. Microbiol. 25: 1043-1047.
  • Maniar A.C., T. Williams, CM. Anad and G.W. Hammond. 1990. Detection of verotoxin in stool specimens. J. Clin. Microbiol. 28: 134-135.
  • Piérard D., G. Cornu and W. Proesmans. 1999. Hemolytic uremic syndrome in Belgium: incidence and association with verocytotoxin-producing Escherichia coli infections. Clin. Microbiol. Infect. 5: 16-22.
  • Ritchie M., S. Partington, J. Jessop and M.T. Kelly. 1992. Comparison of a direct fecal shiga-like toxin assay and sorbitol-MacConkey agar culture for laboratory diagnosis of enterohemorrhagic Escherichia coli infection. J. Clin. Microbiol. 30: 461-464.
  • Sobieszczańska B.M., R. Gryko and C.W. Małek. 2000. Isolation of verotoxigenic strains of Escherichia coli O26 in Poland. Clin. Microbiol. Infect. Dis. 6: 226-231.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-655d12de-9a12-49c0-b206-21367f914cdf
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