SARs on an 835 kb DNA fragment from the Drosophila genome

• Autorzy: Miassod R. , Jullien N.
• Języki publikacji: EN

Abstrakty

EN

We have investigated the loop organization of a 835 kilobases DNA fragment from the Drosophila genome. This analysis has focused on the perodicity of the distribution of anchoring sequences (SARs) and its relationship to the distribution of A,T-rich regions, transcription units, repeated elements, putative replication origins and topoisomerase II cleavege sites. Altogether, the data support the idea of an active participation of SARs to the structural organization and functioning of this eukaryotic genome.

Słowa kluczowe

EN

scaffold attached region; eukaryotic genome; autonomously replicating sequence; Drosophila; replication origin; repeated element; DNA; topoisomerase II

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 2
• Opis fizyczny: p.161-170,fig.

Twórcy

autor

Miassod R.

• Parc Scientifigue de Luminy, Case 907, 13288 Marseille, Cedex 9, France

autor

Jullien N.

Bibliografia

• 1. Surdej, P., Got, C, Rosset, R. & Miassod, R. (1990) Supragenic loop organization: mapping, in Drosophila embryos, of scaffold-associated regions on an 800 kilobase DNA continuum from the 14B-15B first chromosome region. Nucleic Acids Res. 18,3713-3722.
• 2. Surdej, P., Got, C. & Miassod, R. (1990) Develop­mental expression pattern of an 800 kb DNA continuum cloned from the Drosophila X chromosome 14B-15B region. Biol. Cell 68, 105-118.
• 3. Brun, C., Dang, Q. & Miassod, R. (1990) Studies of an 800-kilobase DNA stretch of the Drosophila X chromosome: co-mapping of a subclass of scaffold-attached regions with sequences able to replicate autonomously in Saccharomyces cerevisiae. Mol. Cell. Biol. 10,5455-5463.
• 4. Surdej, P., Brandli, D. & Miassod, R. (1991) Scaffold-associated regions and repeated or cross-hybridizing sequences on an 800 kilobase DNA stretch of the Drosophila X chromosome. Biol. Cell 73,111-120.
• 5. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
• 6. Mirkovitch, J., Mirault, M.E. & Laemmli, U.E. (1984) Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold. Cell 39,223-232.
• 7. Amati, B.B. & Gasser, S.M. (1988) Chromosomal ARS and CEN elements bind specifically to the yeast nuclear scaffold. Cell 54,967-978.
• 8. Struhl, K., Stinchcomb, D.T., Scherer, S. & Davis, R.W. (1979) High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. U.S.A. 76, 1035-1039.
• 9. Rothstein, R. (1985) Cloning in yeast; in DNA cloning: a practical approach (Glover, D.M., ed.) vol. 2, p. 53, IRL Press, Oxford.
• 10. Razin, S.V., Vassetzky, Y.S. & Hancock, R. (1991) Nuclear matrix attachment regions and topoisomerase II binding and reaction sites in the vicinity of a chicken DNA replication origin. Biochem. Biophys. Res. Commun. 177,265-270.
• 11. Earnshaw, W.C. & Heck, M.M.S. (1985) Localization of topoisomerase II in mitotic chromosomes. J. Cell Biol. 100,1706-1715.
• 12. Berrios, M., Osheroff, N. & Fisher, P.A. (1985) In situ localization of DNA topoisomerase II, a major polypeptide component of the nuclear matrix fraction. Proc. Natl. Acad. Sci. U.S.A. 82, 4142-4146.
• 13. Adachi, Y, Kas, E. & Laemmli, U.K. (1989) Preferential, cooperative binding of DNA topoisomerase II to scaffold-associated regions. EMBOJ. 8,3997-4006.
• 14. Wu, H.-Y., Shyy, S., Wang, J.C. & Liu, L.F. (1988) Transcription generates positively and negatively supercoiled domains in the template. Cell 53,433-440.
• 15. Razin, S.V. (1987) DNA interactions with the nuclear matrix and spatial organization of replication and transcription. BioEssays 6,19-23.
• 16. Brewer, B.J. & Fangman, W.L. (1991) Mapping replication origins in yeast chromosomes. BioEssays 13,317-322.
• 17. Bran, C., Surdej, P. & Miassod, R. (1993) Loop organization of the Drosophila rDNA: relations between scaffold-attached regions, sequences replicating autonomously in yeast and a chromosomal replication origin. Exp. Cell Res. 208,104-114.
• 18. Saitoh, Y. & Laemmli, U.K. (1994) Metaphase chromosome structure: bands arise from a differential folding path of the highly AT-rich scaffold. Cell 76,609-622.
• 19. Jackson, D.A. & Cook, P.R. (1993) Transcriptionally active miriichromosomes are attached transiently in nuclei through transcription units. /. Cell Sci. 105,1143-1150.
• 20. Ptashne, M. (1986) Gene regulation by proteins acting nearby and at distance. Nature (London) 697,697-701.
• 21. Ashburner, M. (1989) Drosophila: A Laboratory Handbook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
• 22. Bellen, H.J., Wilson, C. & Gehring, W.J. (1990) Dissecting the complexity of the nervous system by enhancer detection. BioEssays 12,199-204.
• 23. Blumenthal, A.B., Kriegstein, H.J. & Hogness, D.S. (1973) The units of DNA replication in Drosophila melanogaster chromosomes. Cold Spring Harbor Symp. Quant. Biol. 38,205-223.
• 24. Amati, B. & Gasser, S.M. (1990) Drosophila scaffold-attached regions bind nuclear scaffolds and can function as ARS elements in both budding and fission yeast. Mol. Cell. Biol. 10, 5442-5454.
• 25. Bassim Hassan, A., Errington, R.J., White, N.S., Jackson, D.A. & Cook, P.R. (1994) Replication and transcription sites are co-localized in human cells. /. Cell Sci. 107,425-434