Plant regeneration via somatic embryogenesis in Pinus kesiya [Royle ex.Gord.] influenced by triacontanol

• Autorzy: Malabadi R B , Mulgund G.S. , Nataraja K.
• Języki publikacji: EN

Abstrakty

EN

Embryogenic cultures were initiated and established for the first time in 3 different genotypes of Pinus kesiya using mature zygotic embryos and triacontanol. Mature zygotic embryos produced white-mucilaginous embryogenic callus when cultured on half strength MSG (Becwar et al. 1990) basal medium supplemented with 90 mM maltose, 2.0 ·g l⁻¹ Gellan gum, 9.0 μM 2, 4-D and 10 μg·1⁻¹triacontanol. On subculture of such embryogenic callus on the maintenance medium (II) containing 2.0 μM 2,4-D and 2.0 μg ·l⁻¹ triacontanol induced cleavage polyembryogenesis with proembryos. The percentage of somatic embryogenesis was not similar in all the three genotypes. The highest percentage of somatic embryogenesis (88.5 %) was recorded in PK04 genotype. Somatic embryos were successfully germinated on half strength MSG basal medium without growth regulators. Somatic seedlings showed fast growth and a survival rate of 95 %. This work for the first time reveals that triacontanol can be used as an effective growth regulator for inducing somatic embryogenesis in conifers.

Słowa kluczowe

EN

plant regeneration; plant biotechnology; Pinus kesiya; growth regulator; afforestation; somatic embryogenesis

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2005
• Tom: 27
• Numer: 4A
• Opis fizyczny: p.531-537,fig.,ref.

Twórcy

autor

Malabadi R B

• Karnatak University, Pavate Nagar, Dharwad - 58003, Karnatake state, India

autor

Mulgund G.S.

autor

Nataraja K.

Bibliografia

• Becwar M. R., Nagamani R., Wann S. R. 1990. Initiation of embryogenic cultures and somatic embryo development in Loblolly pine (Pinus taeda). Can. J. For. Res. 20: 810 - 817.
• Biernbaum J.A., Houtz R.L., Ries S.K. 1998. Field studi es with crops treated with colloidally dispersed triacontanol. J. Am. Soc. Hort. Sci. 113: 679-684.
• Chibnall A.C., Williams F., Latner A.L., Piper S.H. 1933. The isolation of n-triacontanol from Lucerne wax. Biochem. J. 27: 1885-1888.
• Crosby D.G., Vlitos A.J., 1959. Growth sub stances from Maryland Mammoth T abasco: long chain alcohols. Contrib. Boyce Thompson Inst. 20: 283.
• Fraternale D., Giamperi D.,Ricci D., Rocchi M.B.L. 2002. Microprpagation of Bupleurum fruticosum L.: The effect of triacontanol. Plant Cell Tissue Organ Cult. 69: 135-140.
• Fraternale D., Giamperi D., Ricci D., Rocchi M.B.L., Guidi L., Epifano F., Marcotullio M.C. 2003. The effect of triacontanol on micropropagation and on secretory system of Thymus mastichina. Plant Cell Tissue Organ Cult. 74: 87-97.
• Gupta P. K., Durzan D. J. 1986. Somatic embryo - genesis from caltus of mature sugar pine embryos. Bio/Technology. 4: 643 -645.
• Houtz R.L., Ries S.K.,Tolbert N.E. 1985. Effect of triacontanol on Chlamydomonas. I. Stimulation of growth and photo synthetic CO2 assimilation. Plant Physiol. 79: 357-364.
• Klimaszewska K., Garin E., Cardou M. B., Isabel N., Plourde A. 2000. Effect of sugars, amino acids, and culture technique on maturation of somatic embryos of Pinus strobus on medium with two gellan gum concentrations. Plant Cell Tissue Organ. Cult. 62: 27 - 37.
• Klimaszewska K., Park Y. S., Overton C., Ian, M., Bonga J. M. 2001. Optimized somatic embryogenesis in Pinus strobus L. In Vitro Cell. Dev. Biol-Plant 37: 392-399.
• Kulandaivelu K. 1995. Effect of triacontanol in flooded Erythrina variegata Lam seedlings. Changes in growth, photosynthesis pigments and biomass productivity. Photosynthetica. 31: 269-275.
• Ma F. W., Wang, J. C., Rong W. 1990. Effect of plant growth regulators on in vitro propagation of apple cultivar Fuji. J. Fruit. Sci. 7: 201-206.
• Malabadi R. B., Choudhury H., Tandon P. 2002. Plant regeneration via somatic embryogenesis in Pinus kesiya (Royle ex.Gord). App Biol Res. 4: 1-10.
• Malabadi R. B., Choudhury H., Tandon P. 2003. Effect of gelting agent, carbon sources and sterihzation methods on initiation and establishment of embryogenic cultures in Khasi pine (Pinus kesiya Royle ex. Gord). App Biol Res. 5:1-8.
• Malabadi R. B., van Staden J. 2003. So matic embryos can be induced from the vegetative shoot apex of mature Pinuspatula trees. S Afr J Bot.69:450-451.
• Malabadi R. B., van Staden, J. 2005a. Somatic embryogenesis from vegetative shoot apices of mature trees of Pinus patula. Tree Physiol. 25: 11-16.
• Malabadi, R. B., van Staden, J. 2005b. Role of antioxidants and amino actds on somatic embryogenesis of Pinus patula. In Vitro. Cell. Dev. Biol-Plant. 41: 181-186.
• Mullin T. J., Park Y. S. 1992. Estimating genetic gains from alternative breeding strategies for clonal forestry. Can J of For Res. 22:14-23.
• Nagamani R., Diner A. M., Sharma G. C. 1993. Somatic embryogenesis in long leaf pine (Pinus palustris). Can. J. For. Res. 23: 873 - 876.
• Reddy B.O., Giridhar P., Ravishankar G. A. 2002. The effect of triacontanol on micropropagation of Cap- si cum frutescens and Decalepis hamiltonii W& A. Plant Cell Tissue Organ Cult. 71: 253-258.
• Ries S.K., Wert V. F. 1982. Rapid effects of triacontanol. J. Plant Growth Regul. 1: 117-127.
• Ries S., Houtz R. 1983. Triacontanol as a plant growth regulator. Hort Sci. 18: 654-662.
• Ries S.K, Wert V., Sweeley C. C., Leavitt R.A. 1977. Triacontanol: a new natufally occurring plant growth regulator. Science. 195: 1339-1341.
• Ries S.K., Wert V. F. 1988. Rapid elicitation of second mesfengers by nanomolar doses of triacontanol and octacosanol. Planta. 173: 79-87.
• Srivstava N. K., Sharma S. 1990. Effect of triacontanol onphotosynthesis, alkaloid content and growth in opium poppy (Papaver somniferum L.). Plant Growth Regul. 9: 65-71.
• Stoutemyer V. 1981. Triacontanol: A promising new growth promoting substance. Fuchsia Fan. 41: 9-10.
• Stoutemyer V., Cook R. 1987. Update on Triacontanol. The Orchid Advocate. 13: 86-87.
• Tantos A., Meszaros A., Kissimon J. 1999. The effect of triacontanol on micropropagation of balm, Me lissa officinalis L. Plant Cell Rep. 19: 88-91.
• Tantos A., Meszaros A., Kissimon J., Horvath G., Farkas T. 2001. Triacontanol-supported micropropaf gation of woody plants. Plant Cell Rep. 19: 88-91.
• Yun C., Kim H. I. 1986. Differences in callus formation from different explant sources and effect of plant growth regulators in plant regeneration in rice (Oryza sativa L.). Res. Rep. Rur. Dev. Admin. 1: 113-116.