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2010 | 59 | 2 |

Tytuł artykułu

False negative results in high viremia parvovirus B19-samples tested with real-time PCR

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Extremly high viremia is observed during some viruses infection, especialy in immunocompromised patients. False negative results of Parvovirus B19 DNA tests performed with real-time PCR in high viremic samples are reported. The way of fluorescence diagrams analysis and algorithm of positive result confirmation to exclude such phenomenon are proposed.

Wydawca

-

Rocznik

Tom

59

Numer

2

Opis fizyczny

p.129-132,fig.,ref.

Twórcy

autor
  • Institute of Haematology and Transfusion Medicine, 5th Chocimska Str., 00-957 Warsaw, Poland
autor
autor
autor

Bibliografia

  • Aberham C, C. Pendl, P. Gross, G. Zerlauth and M. Gessner. 2001. A quantitative, internally controlled real-time PCR assay for the detection of parvovirus B19 DNA. J Virol. Method 92: 183-191.
  • Aubin J.T., C. Defer, M. Viduad, M. Maniez Montrenil and B. Flan. 2000. Large-scale screening for human parvovirus B19 DNA by PCR: application to the quality control of plasma for fractionation. Vox Sang. 78: 7-12.
  • Candotti D., N. Etiz, A. Parsyan and J.-P. Allain. 2004. Identification and characterization of persistent human erythrovirus infection in blood donor samples. J. Virol. 78: 12169-12178.
  • Enders M., A. Weidner, T. Rosenthal, C. Baisch, L. Hedman, M. Soderlund-Venermo and K. Hedman. 2008. Improved diagnosis of gestational parvovirus B19 infection at the time of nonimmune fetal hydrops. J. Infect. Dis. 1; 197: 58-62.
  • Kleinman S.H., S.A. Glynn, L. Tzong-Hae, L. Tobler et al. 2007. Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay. Transfusion 47: 1756-64.
  • Koppelman M.H., H.T.M. Cuypers, T. Emrich and H.L.Zaaijer. 2004. Quantitative real-time detection of parvovirus B19 DNA in plasma. Transfusion 44: 97-103.
  • Liefeldt, L., A. Plentz, B. Klempa, O. Kershaw, A.-S. Endres, U. Raab, H-H. Neumayer, H. Meisel and S. Modrow. 2005. Recurrent high level Parvovirus B19/genotype 2 viremia in a renal transplant recipient analysed by real-time PCR for simultaneous detection of genotypes 1 to 3. J. Med. Virol. 75: 161-169.
  • Mackay I.M., K.E. Arden and A. Nitsche. 2002. Real-time PCR in virology. Nucleic Acid Res. 30: 1292-1305.
  • Plentz A., J. Hahn, E. Holler, W. Jilg and S. Modrow. 2004. Long-term parvovirus B19 viraemia associated with pure red cell aplasia after allogeneic bone marrow transplantation. J. Clin. Virol. 21: 16-9.
  • Schmidt M., A. Themann, C. Drexler, M. Bayer, et al. 2007. Blood donor screening for parvovirus B19 in Germany and Austria. Transfusion 47: 1775-1782.
  • Young N.S. and K.E. Brown. 2004. Mechanisms of disease. Parvovirus B19. N. Engl. J. Med. 350: 586-597.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-642adf6a-6ab7-49cc-9de3-f1ce3c6f7258
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