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1998 | 45 | 3 |
Tytuł artykułu

Amino-acid composition of pyruvate kinase M2 isoenzyme variants from rat liver and Morris hepatoma 7777

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Cytosolic fractions B (salted out between 51-70% ammonium sulphate saturation) from rat liver and Morris hepatoma 7777, containing pyruvate kinase (EC 2.7.1.40) M2 isoenzymes, were purified by affinity chromatography on Blue Sepharose CL-6B. When compared by polyacrylamide gel electrophoresis at pH 8.3, all three M2 pyruvate kinase variants from Morris hepatoma 7777 had lower mobilities (α2, β2, γ3) than the three corresponding variants (α1, β1, γ2) from normal rat liver. Using an automatic amino-acid analyser, significant differences in selected amino- acid content have been found in corresponding highly purified γ3 and γ2 variants from Morris hepatoma and normal rat liver, respectively. The γ3 -variant of the Morris hepatoma M2 isoenzyme had twice the amount of L-tyrosine and L-cysteine, and a content of L-serine higher by 20% than the corresponding γ2 variant of the normal rat liver M2 isoenzyme. It contained, however, significantly less dicarboxylic amino acids which explains its lower electrophoretic mobility. It showed also a decrease (by ca. 10%) in several other amino-acid content, corresponding to a 10% decrease in the tumour enzyme molecular mass.
Wydawca
-
Rocznik
Tom
45
Numer
3
Opis fizyczny
p.775-780,fig.
Twórcy
autor
  • Jagiellonian University, Collegium Medicum, Krakow, Poland
autor
autor
Bibliografia
  • 1. Gumiriska, M., Stachurska, M.B. & Ignacak, J. (1988) Biochim. Biophys. Acta 966, 207-213.
  • 2. Gumińska, M. & Ignacak, J. (1996) Biochim. Biophys. Acta 1292, 99-105.
  • 3. Gumińska, M., Stachurska, M.B., Christen­sen, B., Tromholt, V., Kieler, J., Radzikowski, Cz. & Duś, D. (1989) Experientia 45,571-574.
  • 4. Ibsen, K.H. (1977) Cancer Res. 37, 341-353.
  • 5. Gumińska, M. & Ważewska-Czyżewska, M. (1979) Brit. J. Hematol. 41, 125-132.
  • 6. Eigenbrodt, E., Reinacher, M., Scheefers-Bor- chel, U., Scheefers, II. & Friis, R. (1992) CriL Rev. Oncogen. 3, 91-115.
  • 7. Fister, P., Eigenbrodt, E., Presek, P., Rein­acher, M. & Schoner, W. (1983) Biochem. Bio­phys. Res. Com.rn.un. 115, 409-414.
  • 8. Weernink, P.A.O., Rijksen, G., Van der Heij- den, M.C.M. & Staa, G.E.J. (1990) Cancer Res. 50, 4604-4610.
  • 9. Presek, P., Reinacher, M. & Eigenbrodt, E. (1988) FEBS Lett. 242, 194-198.
  • 10. Eigenbrodt, 2., Abdel-Fattah Mostafa, M. & Schöner, W. (1977) Hoppe-Seylcr's Z. Physiol. Chenu 358, 1047-1067.
  • 11. Ignacak, J. & Gumińska, M. (1997) Acta Bio­chim. Polon. 44, 201-208.
  • 12. Gumińska, M., Kędryna, T. & Marchut, E. (1986) Biochem. Pharmacol. 35, 4369-4374.
  • 13. Kędryna, T., Gumińska, M. & Marchut, E. (1990) Biochim. Biophys. Acta 1039,130-133.
  • 14. Ignacak, J. & Gumińska, M. (1993) Acta Bio­chim. Polon. 40, 261-267.
  • 15. Esterday, R.L. & Esterday, I.M. (1974) in Im­mobilized Biochemicals and Affinity Chromatog­raphy (Dunlap, R.B., ed.) pp.123-133, Plenum Press, New York.
  • 16. Laemmli, U.K. (1970) Nature 227, 680-685.
  • 17. Davis, B.J. (1964) Am. N.Y. Acad. Sei. 121, 404-427.
  • 18. Bücher, T. & Pfleiderer, G. (1955) Methods En- zymol 1, 435-440.
  • 19. Gumińska. M„ Ptak, W. & Zembala, M. (1975) Enzyme 19, 24-37.
  • 20. Allen, G. (1981) in Laboratory Techniques in Biochemistry and Molecular Biology (Work, T.S. & Burdon, R.H., cds.) pp. 24-25, North- Holland Publishing Company, Amsterdam, New York, Oxford.
  • 21. Atkin, G.E. & Ferdinand, W. (1970) Analyt. Biochem. 38, 313-381.
  • 22. Dreyer, W.J. & Bynum, E. (1967) Methods En- zymol 11, 35.
  • 23. Lowry, O.H., Rosebrough, N.I., Farr, AL. & Randall, R.I. (1951) J. Biol Chem. 193. 265- 275.
  • 24. Harada, K., Saheki, S., Waola, K. & Tanaka, T. (1978) Biochim. Biophys. Acta 524, 327-339.
  • 25. Saheki, S., Saheki. K. & Tanaka, T. (1982) Bio­chem. Biophys. Acta 704, 484-493.
  • 26. Feliu, I.E., Diaz, I.J., Garcia-Conero, R. & Gosalvez, II. (1975) FEBS Lett. 50, 334-338.
  • 27. Gumińska, M. & PorwitBóbr, Z. (1995) in Mo­lecular Biology for Man and Environment (Gu­mińska, M. & Micdzobrodzki, J., eds.) pp. 127- 142, Jagiellonian University, Cracow.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-article-63b80ad9-317a-4f9f-a70b-a10d3caaabbf
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