Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima

• Autorzy: Huang Z , Long H , Jiang Q-T , Wei Y-M , Yan Z-H , Zheng Y-L
• Języki publikacji: EN

Abstrakty

EN

Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS genes from Ae.longissima Schweinf. & Muschl., a species of the section Sitopsis of the genus Aegilops, which is closely related to the В genome of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the В genome, and the remaining was related to those from A and D genomes of wheat and Ae. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats and Ae. tauschii failed to obtain the expected products, indicating that the 3 groups of LMW-GS genes obtained in this study were new members of LMW-GS multi-gene famailies. These results suggested that the Sitopsis species of the genus Aegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes from Ae. longissima into wheat.

Słowa kluczowe

PL

genetic variation; glutenin; gliadin; wheat storage protein; protein; gluten; subunit; DNA marker; Aegilops longissima; gene; molecular characteristics; Sitopsis; B genome

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2010
• Tom: 51
• Numer: 1
• Opis fizyczny: p.9-18,fig.,ref.

Twórcy

autor

Huang Z

• Dujiangyan Campus, Sichuan Agricultural University, Dujiangyan, Sichuan, China
• Triticeae Research Institute, Sichuan Agricultural University, Yaan, Sichuan, China

autor

Long H

• Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, China

autor

Jiang Q-T

• Triticeae Research Institute, Sichuan Agricultural University, Yaan, Sichuan, China

autor

Wei Y-M

• Triticeae Research Institute, Sichuan Agricultural University, Yaan, Sichuan, China

autor

Yan Z-H

• Triticeae Research Institute, Sichuan Agricultural University, Yaan, Sichuan, China

autor

Zheng Y-L

• Triticeae Research Institute, Sichuan Agricultural University, Yaan, Sichuan, China
• Ministry of Education Key Laboratory for Crop Genetics Resources and Improvement in Southwest China, Sichuan Agricultural University, Yaan, Sichuan, China

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