Transcriptomic profile of two canine mammary cancer cell lines with different proliferative and anti-apoptotic potential

• Autorzy: Krol M. , Pawlowski K.M. , Skierski J. , Rao N.A.S. , Hellmen E. , Mol J.A. , Motyl T.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to identify the genes responsible for the high growth rate and anti-apoptotic potential in selected canine mammary cancer cells. cDNA canine microarrays were used to compare the transcriptome in simple carcinoma CMT-U27 and spindle-cell tumor CMT-U309 cell lines. In CMT-U27 cell line the growth rate (shorter cell cycle), anti-apoptotic potential (higher expression of Bcl-2) was higher and spontaneous and induced apoptosis was lower. Comparison of transcriptomes revealed 333 genes which expression differed similarly. We focused on genes involved in cell proliferation, adhesion and apoptosis, and selected 29 of them. The high growth rate and anti-apoptotic potential in CMT-U27 cells was associated with enhanced expression of genes (at the level of transcripts) involved in Ca2+ signaling pathway (Calmodulin 1, 2, 3 and SPSB2) and growth hormone cellular pathway. The low-proliferative and pro-apoptotic phenotype of CMT-U309 cells was more dependent on TGFß, neuregulin 1 pathways and adhesion-related molecules.

Słowa kluczowe

EN

adhesion; cell line; gene expression; antiapoptotic potential; apoptosis; cDNA microarray; canine mammary cell; cancer cell line; cell proliferation

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2009
• Tom: 60
• Numer: 1
• Opis fizyczny: p.95-106,fig.,ref.

Twórcy

autor

Krol M.

• Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02-776 Warsaw, Poland

autor

Pawlowski K.M.

autor

Skierski J.

autor

Rao N.A.S.

autor

Hellmen E.

autor

Mol J.A.

autor

Motyl T.