Dzialanie in vitro bakterii z rodzaju Lactobacillus izolowanych z pochwy na grzyby wywolujace kandydoze sromu i pochwy

• Autorzy: Strus M. , Brzychczy-Wloch M. , Kucharska A. , Gosiewski T. , Heczko P.B.

Warianty tytułu

EN

Inhibitory activity of vaginal Lactobacillus bacteria on yeasts causing vulvovaginal candidiasis

• Języki publikacji: PL

Abstrakty

PL

Bakterie Lactobacillus delbrueckii, występujące w mikroflorze pochwy wspólnie z gatunkami z grupy Lactobacillus acidophilus, hamują wzrost Candida albicans w czasie 90 minut, zaś bakterie z gatunku Lactobacillus plantarum - po 24 godzinach. Wczesne działanie Lactobacillus można przypisać nadtlenkowi wodoru, zaś późne - innym metabolitom. W warunkach naturalnych bakterie z rodzaju Lactobacillus prawdopodobnie współdziałają ze sobą przeciwko C. albicans, wykorzystując kilka metabolitów.

EN

Growing frequency of therapeutical failures of vulvovaginal candidiasis, resulting from resistance of certain species of Candida to imidazole agents, raises interest in the use of probiotics from Lactobacillus genera as prophylaxis. Unfortunately, little is known about inhibitory mechanisms of Lactobacillus on Candida. The aim of this study was to compare the activity of selected Lactobacillus species, representing the physiological vaginal flora, against Candida as well as investigation whether their inhibitory activity against Candida is related strictly to hydrogen peroxide and lactic acid production. 125 strains from vaginal smears of healthy women were classified by making use of phenotypic and genotypie methods. The majority of strains belonged toL. acidophilus: L. acidophilus sensu stricto, L. crispatus, L. gasseri and L. johnsonii as well as L. fermentům and L. plantarum species. Culture supernatants of selected 25 strains representing the isolated species were examined for their inhibitory activity against the growth of Candida albicans and C. glabrata. The results showed that the strongest and the fastest activity against C. albicans was demonstrated by L. delbrueckii strains, producing the largest quantities of hydrogen peroxide. On the other hand, extended activity, demonstrable after 24 hours, was shown by non-H2O2 producing L. plantarum supernatants. Growth of C. glabrata was not inhibited by any of the examined strains of Lactobacillus. Comparison of activity of live active cultures of Lactobacillus strains and their mixtures with this of pure H2O2 and lactic acid has shown that pure chemical compounds were less active than the cultures. This suggests that mixtures of Lactobacillus strains are in cooperation with each other using many different metabolites.

Słowa kluczowe

PL

choroby czlowieka; kobiety; kandydoza sromu; kandydoza pochwy; czynniki chorobotworcze; grzyby; Candida; Candida albicans; Candida glabrata; Candida krusei; grzybice; bakterie; Lactobacillus delbrueckii; Lactobacillus acidophilus; Lactobacillus plantarum; hamowanie wzrostu; metabolity bakteryjne

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2005
• Tom: 57
• Numer: 1
• Opis fizyczny: s.7-17,rys.,tab.,bibliogr.

Twórcy

autor

Strus M.

• Collegium Medicum Uniwersytetu Jagiellonskiego, ul.Czysta 18, 31-121 Krakow

autor

Brzychczy-Wloch M.

autor

Kucharska A.

autor

Gosiewski T.

autor

Heczko P.B.

Bibliografia

• 1. Antonio MA, Hawes SE, Hillier SL. The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis 1999; 180: 1950-6.
• 2. Bohalcl, Back W, Richter L i inni. Lactobacillus amylolyticus sp. nov., isolated from beer malt and beer wort. Syst Appl Microbiol 1998; 21: 360-4.
• 3. Falsen E,Pascual C, Sjoden B, Ohlen M, Collins MD. Phenotypic and phylogenetic characterisation of a novel Lactobacillus species from human sources: description of Lactobacillus iners sp. nov. Int J Syst Bacteriol 1999; 449: 217-21.
• 4. Ferrer J. Vaginal candidosis: epidemiological and etiological factors. Int J Gynaecol Obstet 2000; 71: 21-7.
• 5. Fidel PL. Distinct protective host defenses against oral and vaginal candidiasis. Med Mycology 2002; 40: 359-75.
• 6. Fujisawa T., Benno Y.,Yaeshima T., Mitsuoka T. Taxonomie study of the Lactobacillus acidophilus group, with recognition of Lactobacillus gallinarum sp. nov. and synonymy of Lactobacillus acidophilus group A3 (Johnson et al. 1980) with the type strain of Lactobacillus amylovorus (Nakamura 1981). Int J Syst Bacteriol 1992; 42:487-91.
• 7. Hilton E., Isenberg HD, Alpersein P i inni. Ingestion of yogurt containing Lactobacillus acidophilus as prophylaxis for candidal vaginitis. Ann Intern Med 1992; 116: 353-7.
• 8. Horiguchi H, Yurimoto H, Goh T i inni. Peroxidal catalase in the methylotrophic yeast Candida boidii: transport efficiency and metabolic significance. J Bacteriol 2001; 183: 6372-83.
• 9. Johnson JL, Phelps CF, Cummins CS i inni Taxonomy of the Lactobacillus acidophilus group. Int J Sys Bacteriol 1980; 30: 53-68.
• 10. Kmet V, Lucchini F. Aggregation-promoting factor in human vaginal Lactobacillus strains. FEMS Immunol Med Microbiol 1997; 19: 111-4.
• 11. Nugent RP, Krohn MA, Hillier SL. Reliability of diagnosing bacterial vaginosis is improved by a standardized method of Gram stain interpretation. J Clin Microbiol 1991; 29: 297-301.
• 12. Onderdonk AB, Zamarchi GR, Rodriguez ML i inni. Qualitative assessment of vaginal microflora during use of tampons of various compositions. Appl Environ Microbiol 1987; 53: 2779-84.
• 13. Shalev E, Battino S, Weiner E inni. Ingestion of yogurt containing Lactobacillus acidophilus compered with pasteurized yogurt as prophylaxis for recurrent candidal vaginitis and bacterial vaginosis. Arch Fam Med 1966; 5: 593-6.
• 14. Strom K, Sjogren J, Broberg A, Schnurer J. Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides Cyco (L-Phe-L-Pro) and Cyclo (L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid. Appl Environ Microbiol 2002; 68: 4322-7.
• 15. Strus M, Brzychczy-Włoch M, Kochan P, Heczko PB. Nadtlenek wodoru, produkowany przez bakterie z rodzaju Lactobacillus, jako czynnik regulujący mikroflorę pochwy. Med Dośw Mikrobiol 2004; 56: 67-77.
• 16. Strus M, Malinowska M, Heczko PB. In vitro antagonistic effect of Lactobacillus on organisms associated with bacterial vaginosis. J Reprod Med 2002; 47: 41-6.
• 17. Tilsaia-Timisjarvi A, Alotossava T. Development of oligonucleotide primers from the 16S-23S rRNA intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR. Int J Food Microbiol 1997; 35: 49-56.
• 18. Vasquez A, Jakobsson T, Ahme S i inni. Vaginal Lactobacillus flora of healthy Swedish women. J Clin Microbiol 2002; 40: 2746-9.
• 19. Walter J, Tannock GW, Tilsala-Timisjarvi A. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and specific-species PCR primers. Appl Environ Microbiol 2000; 66: 297-303.
• 20. White TC, Holleman S, Dy F i inni Resistance mechanisms in clinical isolates of Candida albicans. Antimicrob Agents Chemother 2002; 46: 1704-13.