Wybrane parametry odpornosci nieswoistej u indykow szczepionych Dindoralem-SPF po podaniu lewamizolu i izoprynozyny

• Autorzy: Wojcik R , Swiecicka-Grabowska G.

Warianty tytułu

EN

Selected parameters of non-specific immunity in turkeys vaccinated with Dindoral-SPF in response to a NDV antigen after the administration of levamisol and isoprinozine

• Języki publikacji: PL

Abstrakty

EN

The aim of the present study was to compare selected biochemical and non-specific immunity parameters in turkeys vaccinated with Dindoral-SPF in response to a live virus antigen, the Roakin strain of Newcastle disease virus (NDV), after the administration of two synthetic immunity stimulators: isoprinozine and levamisol. The experiment was performed on 30 turkeys. At 24-days-of-age they were vaccinated with the commercial vaccine Dindoral-SPF containing an attenuated strain of hemorrhagic enteritis virus (HEV). At 29-days-of-age the turkeys were divided into three groups of ten birds each. Particular subgroups were given a single dose of either levamisol or isoprinozine, and the third group (the control) received none of the immunity stimulators. After 7 days and again after 100 days all birds received a live mesogenic Roakin strain of NDV. Biochemical and non-specific immunity parameters were determined in blood collected from the wing vein. The results obtained indicated short-term but statistically significant decreases and increases in the levels of biochemical parameters (total protein, gammaglobulins and ceruloplasmin) and non-specific immunity indicators (lysozyme activity) in both experimental groups, as compared with the control group. More interesting, indicating higher effectiveness of isoprinozine than levamisol, were the results concerning non-specific cellular immunity in turkeys, including a higher metabolic activity of leukocytes in the NBT test, especially during the primary response, and higher leukocyte reactivity in the TB test, reflected by RI values, especially during the secondary response. The phagocytic activity of leukocytes, expressed as a percentage of phagocytizing cells and the values of the phagocytic index, was the highest in isoprinozine-stimulated turkeys

Słowa kluczowe

PL

indyki; immunostymulatory; zwierzeta szczepione; wskazniki odpornosci nieswoistej; lewamizol; izoprynozyna; drob; odpornosc nieswoista

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 11
• Opis fizyczny: s.1256-1260,tab.,bibliogr.

Twórcy

autor

Wojcik R

• Uniwersytet Warminsko-Mazurski, ul.Oczapowskiego 13, 10-957 Olsztyn

autor

Swiecicka-Grabowska G.

Bibliografia

• 1.Balla L., Papocsi L., Szurop I., Toth B.: Studies on the efficiency of various immunization methods and schedules against Newcastle disease. I. Immune state of chicken flocks after repeated drinking water and aerosol vaccinations with the LaSota strain. Acta Vet. Acad. Sci. Hung. 1979, 27, 253-263.
• 2.Cardona C. J., Reed W. M., Witter R. L., Silva R. F.: Protection of turkeys from hemorrhagic enteritis with a recombinant fowl poxvirus expressing the native hexon of hemorrhagic enteritis virus. Avian Dis. 1999, 43, 234-244.
• 3.Delafuente J. C., Panush R. C.: Pharmacologic immunoenhancement in the elderly: in vitro effects of isoprinosine. Clin. Immunol. Immunopathol. 1988, 47, 363-367.
• 4.Domermuth C. H., Gross W. B.: Effect of disinfectants and drying on the virus of hemorrhagic enteritis of turkeys. Avian Dis. 1971, 15, 94-97.
• 5.Domermuth C. H., Gross W. B.: Effect of chlorine on the virus of hemorrhagic enteritis of turkeys. Avian Dis. 1972, 16, 952-953.
• 6.Domermuth C. H., Gross W. B., Douglass C. S., DuBose R. T., Harris J. R., Davis R. B.: Vaccination for hemorrhagic enteritis of turkeys. Avian Dis. 1977, 21, 557-565.
• 7.Flaming K. P., Blecha F., Fedorka-Cray P. J., Anderson G. A.: Influence of isoprinosine on lymphocyte function in virus-infected feeder pigs. Am. J. Vet. Res. 1989, 50, 1653-1657.
• 8.Goch J. H., Tchórzewski H., Niedworak J., Tkaczewski W., Ofierska H., Soszyñska W.: Test transformacji blastycznej limfocytów i zahamowania migracji leukocytów w przebiegu przewlekłego wirusowego zapalenia miêęśnia sercowego u ludzi. Immunol. Pol. 1981, 6, 239-248. 9.Guiro S.: Badania nad immunosupresyjnąroląwirusa krwotocznego zapalenia jelit u indyków. Praca dokt. UWM, Olsztyn 2000.
• 10.Hennessy K. J., Blecha F., Pollman D. S., Kluber E. F.: Isoprinosine and levamisole immunomodulation in artificially treared neonatal pigs. Am. J. Vet. Res. 1987, 48, 477-480.
• 11.Koncicki A., Krasnodębska-Depta A.: Aktualne problemy w patologii indyków w Polsce. Mat. VIII Symp. Drob., Polanica Zdrój 1997, s. 67-69.
• 12.Larski Z., Grabowska G., Spohr-Foundez I.: Poziom przeciwciał HI i indeks hamowania migracji leukocytów u kurcząt po dwustopniowym doustnym szczepieniu przeciw chorobie Newcastle. Med. Weter. 1977, 33, 153-155.
• 13.Le Gros F. X., Gillet J. P., Toquin D., Guittet M., Bennejean G.: Etude de l'effet immunodépresseur de souches virulente ou vaccinale de l'entérite hémorragique de la dinde. Avian Path. 1988, 17, 547-558.
• 14.Lowry O. H., Rosebrough N. J., Farr A. L., Randall R.: Protein measurements with the folin phenol reagent. J. Biol. Chem. 1951, 193, 265-275.
• 15.Minta Z., Koncicki A.: Nowe jednostki chorobowe u indyków. Mat. III Międzynarodowego Symp. PRO ANIMALI '96, 1996, s. 5-7.
• 16.Neumann U., Sander I., Rautenschlein S., Behr H. P., Baron G.: Krwotoczne zapalenia jelit u indyków. Aspekty patogenezy i uodpornienia. Mat. VIII Symp. Drob., Polanica Zdrój 1997, s. 70-77.
• 17.Parry R. M., Chandau R. C., Shahani R. M.: A rapid and sensitive assay of muramidase. Proc. Soc. Exp. Biol. Med. 1965, 119, 384-386.
• 18.Rautenschlein S., Sharma J. M.: Response of turkeys to simultaneous vaccination with hemorrhagic enteritis and Newcastle disease viruses. Avian Dis. 1999, 43, 286-292.
• 19.Rice E. W., Wagman E., Takenaka Y.: Ceruloplazmin assay in serum: standardization of ceruloplazmin activity in terms of international enzyme units. Diag. Lab. 1986, 12, 39-53.
• 20.Sander I., NeumannU., Lindloff-Muller J.: Vaccination against adenovirus (type II) induced hemorrhagic enteritis in turkey flocks: field trials and laboratory experiments. Mat. XIth International Congress of the World Veterinary Poultry Association. Budapest 1997, s. 43.
• 21.Sauders G. K., Pierson F. W., Van den Hurk J. V.: HEV infection in turkeys: a comparison of virulent and avirulent virus infections and a proposed pathogenesis. Avian Dis. 1993, 22, 47-58.
• 22.Siwicki A. K., Anderson D. P.: Immunostimulation in fish: Measuring the effects of stimulants by serological and immunological methods. U.S. Fish Wildl. Service-IFI, Olsztyn 1993, 1, 17.
• 23.Studnicka M., Dunier M., Siwicki A. K., Morand M.: Aplication of biostimulants in rectifing of immunological reactivation handicapped by phosphoroorganic pesticides, [w:] Siwicki A. K. (red.): Biologiczne monitorowanie skażenia śœrodowiska. Olsztyn, IRS 1997, 187-194.
• 24.Suresh M., Sharma J. M.: Hemorrhagic enteritis virus induced changes in the lymphocyte subpopulations in turkeys and the effect of experimental immunodeficiency on viral pathogens. Vet. Immunol. Immunopathol. 1995, 45, 139-150.
• 25.Sychłowy A., Lukas A.: Ocena mikroilościowej metody redukcji NBT przez granulocyty krwi obwodowej. Pol. Tyg. Lek. 1978, 33, 45-48.
• 26.Wiśniewski J., Grabowska G., Trybała E., Rotkiewicz Z.: Wpływ podania biotropiny i lewamizolu na wybrane wskaźniki odporności swoistej i nieswoistej u świñ. Medycyna Wet. 1993, 49, 66-68.
• 27.Wiśniewski J., Grabowska G., Wasilewska A.: Badanie immunosupresyjnego działania szczepu LaSota wirusa choroby Newcastle (NDV) u kurcząt. Medycyna Wet. 1982, 38, 41-43.
• 28.Wójcik R., Święcicka-Grabowska G.: Parametry odporności swoistej u indyków szczepionych wirusem NDV po podaniu lewamizolu lub izoprynozyny. Medycyna Wet. 2003, 59, 808-810.
• 29.Wójcik R., Święcicka-Grabowska G., Siwicki A. K.: Wpływ lewamizolu i izoprynozyny na odpornoœść nieswoistą u indyków supresorowanych karbarylem. Medycyna Wet. 2004, 60, 437-440.