Method of parenchyma cells parametrisation using fluorescence images obtained by confocal scanning laser microscope

• Autorzy: Zdunek A. , Umeda M. , Konstankiewicz K.
• Języki publikacji: EN

Abstrakty

EN

Fruits and vegetables are built mainly of thin walled parenchyma cells highly susceptible to mechanical damage. According to experimental investigation of relationship between cells geometrical parameters and mechanical properties of tissue a new efficient method of structure reconstruction and parametrisation is elaborated and presented in this paper. Special sample preparation and way of image taking by Confocal Scanning Laser Microscope with fluorescence mode allows obtaining images of any number of cells. Unique computer procedure based on morphological operators was created that allows analysing automatically any number of images with extracting geometrical parameters of each cell separately. The method was tested on potato and carrot tissue. Results show that the method is fast and gives proper cell reconstruction for both materials. In order to decrease error of incorrect reconstruction, possibility of manual correction was introduced to the procedure.

Słowa kluczowe

EN

cell size; geometrical parameter; mechanical damage; plant tissue; cell shape; parenchyma cell; mechanical property; vegetable; microscope image; fruit; image analysis

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Agricultural Engineering
• Rocznik: 2004
• Tom: 07
• Numer: 1
• Opis fizyczny: http://www.ejpau.media.pl/series/volume7/issue1/engineering/art-01.html

Twórcy

autor

Zdunek A.

• Polish Academy of Sciences, 4 Doswiadczalna Street, 20-290 Lublin, Poland

autor

Umeda M.

autor

Konstankiewicz K.

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