Embryogenic callus induction and differentiation in silver fir (Abies alba Mill.) tissue cultures

• Autorzy: Nawrot-Chorabik K
• Języki publikacji: EN

Abstrakty

EN

The research was conducted on explants of silver fir (Abies alba Mill.) deriving from several forest districts in southern Poland. The study encompassed the influence of the origin of plant material, type of explants, kind of substances used for explants sterilization, PPM and the type of medium on the ability to form embryogenic callus and to develop somatic embryos in silver fir explants. From the plant material collected in three sites, 57 clones were obtained from mature zygotic embryos; this produced an embryogenesis frequency of 6%. Embryogenic callus was obtained with a diameter of 65–70 mm depending on the material origin. The best medium for development of callus inducted on embryos isolated from mature silver fir seeds was the SH medium. Somatic embryos were formed in a globular stadium (24 pieces) on this medium. The 10% solution of NaOCl (used for 15 minutes) turned out to be the most effective substance for seed sterilization.

Słowa kluczowe

EN

silver fir; Abies alba; tissue culture; embryogenic suspensor mass; somatic embryogenesis; callus induction; differentiation

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2008
• Tom: 59
• Opis fizyczny: p.31-40,fig.,ref.

Twórcy

autor

Nawrot-Chorabik K

• Agriculture University, Al.29-Listopada 46, 31-425 Krakow, Poland

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