A potent chitinolytic activity of Alternaria alternata isolated from Egyptian black sand

• Autorzy: Sharaf E F
• Języki publikacji: EN

Abstrakty

EN

Eight fungal species characterized by chitinolytic activity were isolated from Egyptian black sand collected from Rosetta coast. Genus Aspergillus and Alternaria alternata exhibited the highest density (> 40% of the total count, each) on the isolation plates containing different treatments of native shrimp shell chitin. Genus Aspergillus was represented by A. flavus, A. niger, A.foetidus and A. ungius, with the former species being the most dominant. The other species were Cladosporium herbarum, Fusarium equisitum (5.71% of the total count, each) and Dendryphiella vinosa (3.21% of the total count). The isolated species were screened for chitinase production on agar plates containing 0.2% colloidal chitin. The chitinolytic activity of each individual was not always correlated with its density on the isolation plates. Alternaria alternata was the most promising species for chitinase excretion. The use of colloidal chitin (1.5%) as a sole carbon source was superior for the enzyme production by A. alternata. Maximum enzyme yield was obtained after 7 days incubation at 30°C with shaking (150 rev min⁻¹), with an initial pH value of the growth medium at 5.0. Presence of NaN0₃ (0.3%), the best nitrogen source, and CaCl₂ (100 pg/ml) stimulated the induction of the enzyme. The crude A. alternata chitinase revealed a potential insecticidal effect on the larvae of fruitfly (82% mortality) and could degrade crude shrimp shell waste.

Słowa kluczowe

EN

Fusarium equisitum; Aspergillus foetidus; Aspergillus ungius; microbiology; Egyptian black sand; Aspergillus flavus; Alternaria alternata; Aspergillus; Cladosporium herbarum; chitinolytic activity; Dendryphiella vinosa; fungi species; Aspergillus niger; chitinase

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2005
• Tom: 54
• Numer: 2
• Opis fizyczny: p.145-151,fig.,ref.

Twórcy

autor

Sharaf E F

• Cairo University, Giza 12613, Egypt

Bibliografia

• Ali U.F. and Z.M. Ibrahim. 2003. Chitinolytic activity of the thermophilic fungi Chaetomium thermophilum and Thermomyces lanuginosus. N. Egypt. J. Microbiol. 5: 80-90.
• Chang K.L.B. and G. Tsai. 1997. Response, surface optimization and kinetics of isolating chitin from pink shrimp (Solenocera melantho) shell waste. J. Agric. Food Chem. 45: 1900-1905.
• Cosio J.G., R.A. Fisher and RA. Carroad. 1982. Bioconversion of shell-fish chitin waste: waste pretreatment, enzyme production, process, design and economic analysis. J. FoodSci. 47: 901-905.
• Dabbour G.A. 1995. Estimation of the economic minerals reserves in Rosetta beach sands. Egypt. Mineral. 7: 1-6.
• Donzelli B.G. and G.E. Harman. 2001. Interaction of ammonium, glucose and chitin regulates the expression of cell wall degrading enzymes in Trichoderma atroviride strain Pl. Appl. Environ. Microbiol. 67: 5643-5647.
• Ellis M.B. 1971. Dematiaceous Hyphomycetes. Commonwealth Mycol. Inst. Kew. Surey England, p. 494.
• Ellis M.B. 1976. More Dematiaceous Hyphomycetes. Commonwealth Mycol., Inst. Kew. Surey England, p. 595.
• El-Naghy M.A., S.K.M. Hassan and A.M. El-Shahed. 1985. Chitin digestion by some soil fungi. Bull. Fac. Sci. Assiut Univ. 14: 33.
• Fenton D.M. and D.E. Eveleigh. 1981. Purification and mode of action of a chitosanase from Pénicillium islandicum. J. Gen. Microbiol. 126: 151-156.
• Ferrer J., G. Palz, Z. Marmol, E. Ramones, H. Garcia and L. Forster. 1996. Acid hydrolysis of shrimp-shell wastes and the production of single cell protein from the hydrolyzate. Biore. Techn. 57: 60-64.
• Gad A.S. 2003. Production of chitinase from Bacillus subtilius in solid state culture. N. Egypt. J. Microbiol. 5: 112-124.
• Ghaly M.F. 2003. Improvement of the activity of cell wall degrading chitinase produced by Streptomyces albovinaceus and its uses as antifungal and insecticide. Egypt. J. Biotechnol. 14: 374-382.
• Ghareib M., M.M. Nour El-Dein, M.A. Abbas and G.G. El Diasty. 2004. Purification and characterization of chitinase from Cunnighamella echinulata. N. Egypt. J. Microbiol. 7: 190-197.
• Giambattesta G.R., F. Federici, M. Petruccioli and M. Fenice. 2001. The chitinolytic activity of P. janthinellum P9: Purification, partial characterization and potential applications. J. Appl. Microbiol. 91: 498-505.
• Haran S., H. Schikler, A. Oppenheim and I. Chet. 1995. New components of the chitinolytic system of Trichoderma harzianum. Mycol. Res. 99: 441-445.
• Jeuniaux C. 1966. Chitinases. Methods Enzymol. 8: 644-651.
• Johnson L.F., E.A. Curl, J.H. Bond and H.A. Fribourg. 1960. Methods for Studying Soil Microflora-Plant Disease Relationship. Burgess Publishing. Minneapolis. U.S.A. pp. 178.
• Kapat A., S.K. Rakshit and T. Panda. 1996. Parameters optimization of chitin hydrolysis by Trichoderma harzianum chitinase under assay condition. Bioprocess Engin. 14: 275-279.
• McCormack J., T.J. Hackett, M.G. Tuohy and M.P. Coughlan. 1991. Chitinase production by Talaromyces emersonii. Biotechnol. Lett. 13: 677-681.
• Mendosa E., P. Vartak, J. Rao and M. Deshpande. 1996. An enzyme from Myrothecium verrucaria that degrades insect cuticle for biocontrol of Aedes aegypti mosquito. Biotech. Lett. 18: 373-376.
• Mercer CF., D.R. Greenwood and J.L. Grant. 1992. Effect of plant and microbial chitinases on the eggs and juveniles of Meloidogyne hapta chitwood. Nematologica 38: 227-236.
• Monreal J. and E.T. Reese. 1969. The chitinase of Serratia marcescens. Can. J. Microbiol. 15: 689-696.
• Moubasher A. 1993. Soil Fungi in Qatar and Other Arab Countries. Sci. Appl. Res. Center Univ. Qatar, p. 566.
• Muzzarelli R.A. 1977. Chitin. Pergamon Press, New York, N.Y., p. 309.
• Nopakarn P., P. Abhinya, S. Yano, W. Marunu and T. Takashi. 2002. Utilization of shrimp shellfish waste as a substrate for solid-state cultivation of Aspergillus sp. S1-13. J. Biosa. Bioeng. 93: 550-556.
• Nour El-Dein M.M., M. Abbas and G.G. El-Diasty. 1999. Regulation of chitinase production in Cuninghamella echinulata. Egypt. J. Bot. 39: 15-26.
• Palani P.V. and D. Lalithakumari. 1999. Inhibition of Venturia inaequalis by genetically improved Trichoderma logbrachiatum strains. Zeitschrift fur Pflanzen Krankheiten und Pflanzencturtz 106: 460-4654.
• Patil R., V. Ghormade and M. Deshpande. 2000. Chitinolytic enzymes: an exploration. Enz. Microbiol. Techn. 26: 473-783.
• Raper K.B. and D. Fennell. 1965. The genus Aspergillus. Williams and Wilkins, Baltimore, U.S.A.
• Reguera G. and S.B. Leschine. 2003. Biochemical and genetic characterization of Chi A, the major enzyme component for the solubilization of chitin by Cellulomonas uda. Arch. Microbiol. 18: 434-443.
• Reid J.D. and D.M. Orgrydiazk. 1981. Chitinase-over producing mutant of Serrada marcescens. Appl. Environ. Microbiol. 41: 664-468.
• Reissig J.L., J.L. Strominger and L.F. Leloir. 1955. A modified colorimetric method for estimation of N-acetylamino sugar. J. Biol. Chem. 27: 959-962.
• Rodriguez J., J.L. Copa-Patino and M.I. Perez-Leblic. 1995. Purification and properties of a chitinase from Penicillium oxalicum autolysates. Lett. Appl. Microbiol. 20: 46-51.
• Saad B. and H.S. Hamdy. 2004. Purification and some properties of the extracellular chitinase produced by Paecilomyce variotii. Egypt. J. Biotechnol. 16: 150-161.
• Sakai K., A. Yokota, H. Kurokawa, M. Wakayama and M. Moriguchi. 1998. Purification and characterization of three thermostable endochitinases of a noble Bacillus strain MH-I, isolated from chitin-containing compost. Appl. Environ. Microbiol. 64: 3397-3402.
• Sherief A.A., M.M. El-Sawah and M.A. Abdel-Naby. 1991. Some properties of chitinase produced by a potent Aspergillus carneus. Appl. Microbiol. Biotechnol. 35: 228-230.
• Shindia A. A., M.I. Abou El Hawa and K.El-S.M. Shalaby. 2001. Fungal degradation of shrimp shell chitin waste. I. Effects of culture conditions on the production of chitinase by a potent Trichoderma viride. Egypt. J. Microbiol. 36: 119-134.
• Taylor G., J. Suha, M. Pierre and K. Wagahatullam. 2002. Purification and characterization of an extracellular exochitinase, B-n-cetylhexosaminidase, from the fungal mycoparasite Stachybotrys elegans. Can. J. Microbiol. 48: 311-319.
• Tweddell R.J., S.H. Habji-Hare and M.C. Pierre. 1994. Production of chitinase and B-1,3-glucanases by Stachybotrys elegans, a mycoparasite of Rhizoctonia solani. Appl. Environ. Microbiol. 60: 489-593.
• Ulhoa C.J. and J.F. Peberdy. 1991. Regulation of chitinase synthesis in Trichoderma harzianum. J. Gen. Microbiol. 137: 2162-2167.
• Witkowska D. and A. Maj. 2002. Production of lytic enzymes by Trichoderma spp. and their effect on the growth of phyto-pathogenic fungi. Folia Microbiol. 47: 279-282.