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2007 | 63 | 04 |

Tytuł artykułu

Fenotypowa charakterystyka szczepow Malassezia pachydermatis

Warianty tytułu

EN
Phenotypic characteristics of Malassezia pachydermatis strains

Języki publikacji

PL

Abstrakty

EN
Yeast like fungi of Malassezia pachydermatis species are numbered among the opportunistic agents accounting for acute dermatoses or even systemic infections in people and animals. They exhibit the clear heterogeneity pertaining to the phenotypic traits and growth requirements. The objective of the present study was to determine the degree of variation as well as the biochemical profiles of M. pachydermatis strains. The studies were conducted on 40 strains isolated from the auditory canal of healthy dogs and those with otitis externa symptoms recognized. An assessment was conducted of the traits of fungus morphology (size and shape of cells and colony type), expression of extracellular hydrolases (API-ZYM) and the activity of catalase, urease, caseinase, beta-glucosidase, phospholipase and Tween hydrolases: 20, 40, 60 and 80. The obtained results made it possible to determine the general metabolism pattern for this species: no fermentability or capacity for assimilation of most carbohydrates, poor proteolytic properties, high activity of enzymes from the phosphatase and lipolytic enzymes groups. On the grounds of the statistical analysis, the examined strains were classified into 7 separate groups of congenial morphology and a determined level of enzymatic activity. Biotype I includes large, smooth and creamy-white colonies of high metabolic activity; moreover, they exhibit high sensitivity to the inhibitory operation of Tween 20 and Cremophor EL; biotype II exhibits a rough type of colony growth, the lowest metabolic activity and a lack of expression of lipase C14 and enzymes from the arylamidase group; biotype III comprises the strains of large, smooth colonies, poor total enzymatic activity and with no activity of cystynic arylamidase; biotype IV includes the strains of small and smooth colonies, average metabolic activity but with optimal usability of Tween 40 and 60 hydrolysis products for growth; biotype V groups the strains of the average metabolic activity and without phospholipase activity recorded; biotype VI comprises the strains of smooth, large or medium-sized colonies and a relatively high enzymatic activity as well as the highest level for alkaline phosphatase and valine arylimidase, phospholipase, catalase, caseine, Tween 80 hydrolase; biotype VII is characterized by the highest total enzymatic activity, high capacity for eskulin break-down, Tween 40, 60 and 80 hydrolysis. Further studies are needed to demonstrate a correlation between the strain classification into a defined biotype and its ecologic or clinical status.

Wydawca

-

Rocznik

Tom

63

Numer

04

Opis fizyczny

s.448-454,tab.,bibliogr.

Twórcy

  • Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-033 Lublin

Bibliografia

  • 1.Aspiroz C., Ara M., Varea M., Rezusta A., Rubio C.: Isolation of Malassezia globosa and Malassezia sympodialis from patients with pityriasis versicolor in Spain. Mycopathologia 2001, 154, 111-117.
  • 2.Bond R., Anthony R. M.: Characterization of markedly lipid-dependent Malassezia pachydermatis isolates from healthy dogs. J. Appl. Bacteriol. 1995, 78, 537-542.
  • 3.Bond R., Lamport A. J., Lloyd D. H.: Colonization status of Malassezia pachydermatis on the hair and in the hair follicle of healthy beagle dogs. Res. Vet. Sci. 2000, 68, 291-293.
  • 4.Breuer-Strosberg R., Hocheithner M., Kuttin E. S.: Malassezia pachydermatis isolation from a scarlet mascaw. Mycoses 1990, 33, 247-250.
  • 5.Crespo M. J., Abarca M. J., Cabanes F. J.: Occurence of Malassezia spp. in horses and domestic ruminants. Mycoses 2002, 45, 333-337.
  • 6.Crespo M. J., Abarca M. L., Cabanes F. J.: Atypical lipid-dependent Malassezia species isolated from dogs with otitis externa. J. Clin. Microbiol. 2000, 38, 2383-2385.
  • 7.David M., Gabriel M., Kopecka M.: Unusual ultrastructural characteristic of yeast Malassezia pachydermatis. Scr. Med. 2003, 76, 173-186.
  • 8.Duarte E. R., Melo M. M., Hahn R. C., Hamdan J. S.: Prevalence of Malassezia species in the ears of asymptomatic cattle with otitis in Brasil. Med. Mycology 1999, 37, 159-162.
  • 9.Gueho E., Boeghout T., Ashbee H. R., Guillot J., Van Belkum A., Faergemann J.: The role of Malassezia species in the ecology of human skin and as pathogens. Med. Mycology 1998, 36, 220-229.
  • 10.Gueho E., Midgley G., Guillot J.: The genus Malassezia with description of four new species. Antonie Van Leeuwenhoek 1996, 68, 337-355.
  • 11.Guillot J., Bond R.: Malassezia pachydermatis: a review. Med. Mycology 1999, 37, 295-306.
  • 12.Guillot J., Gueho E., Lesourd M., Midgley G., Chevrier G., Dupont B.: Identification of Malassezia species: A practical approach. J. Mycol. Med. 1996, 6, 133-110.
  • 13.Guillot J., Gueho E.: The diversity of Malassezia yeasts confirmed by rRNA sequence and nuclear DNA comparisons. Antonie Van Leeuwenhoek 1995, 67, 297-314.
  • 14.Gupta A. K., Kohli Y., Summerbell R. C.: Molecular differentiation of seven Malassezia species. J. Clin. Microbiol. 2000, 38, 1869-1875.
  • 15.Gupta A. K., Kohli Y., Summerbell R. C., Faergemann J.: Quantitative culture of Malassezia species from different body sites of individuals with or without dermatoses. Med. Mycology 2001, 39, 243-251.
  • 16.Inamader A. C., Palit A.: The genus Malassezia and human disease. Indian J. Dermatol. 2003, 69, 265-270.
  • 17.Kesavan K. S., Holland K. T., Ingham E.: The effects of lipid extraction on the immunomodulatory activity of Malassezia spp. in vitro. Med. Mycology 2000, 38, 239-247.
  • 18.Kiss G., Radvanyi S., Szigeti G.: Characteristic of Malassezia pachydermatis strains isolated from canine otitis externa. Mycoses 1996, 39, 313-321.
  • 19.Król J., Staroniewicz Z.: Ocena aktywności enzymatycznej grzybów drożdżopodobnych Malssezia pachydermatis izolowanych od psów. Mykologia Lek. 2000, 7, 7-12.
  • 20.Mancianti F., Rum A., Nardoni S., Carazza M.: Extracellular enzymatic activity of Malassezia species. Mycopathologia 2000, 149, 131-135.
  • 21.Mickelsen P. A., Viano-Paulson M. C., Stevens D. A., Diaz P. S.: Clinical and microbiological features of infection with Malassezia pachydermatis in highrisk infant. J. Infect. Dis. 1988, 6, 1163-1168.
  • 22.Nakagaki K., Hata K., Iwata E., Takeo K.: Malassezia pachydermatis isolated from a South American Sea Lion with dermatitis. J. Vet. Med. Sci. 2002, 62, 901-903.
  • 23.Plotkin L. I., Squiquera L., Mathov I., Galimberti R., Leoni J.: Characterization of the lipase activity of Malassezia furfur. J. Med. Vet. Mycol. 1996, 34, 43-48.
  • 24.Ramsay I. O., Silverman B. W.: Applied functional data analysis. Method and case study. Springer Verlag, Nowy Jork 2002.
  • 25.Shattuck K. E., Cochran C. K., Zabrausky R. J., Pasarell L., Davis J. C., Malloy M. H.: Colonization and infection associated with Malassezia and Candida species in a neonatal unit. J. Hosp. Infect. 1996, 34, 123-129.
  • 26.Tuleva B., Vasileva-Tonkova E., Galabova D.: A specific alkaline phosphatase from Saccharomyces cerevisiae with protein phosphatase activity. FEMS Microbiol. Lett. 1998, 161, 139-144.
  • 27.Vasilieva-Tonkova E., Balasheva D. M., Galabova D.: Influence of growth temperature on the acid phosphatase activity in the yeast Yarrowia lipolytica. FEMS Microbiol. Lett. 1996, 145, 267-271.
  • 28.Wołoszyn S., Winiarczyk S.: Rola grzybów z rodzaju Pityrosporum w patogenezie schorzeń skórnych. Medycyna Wet. 1986, 42, 131-135.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-604f1294-93c2-4c96-8e8b-ffd69b18cc4a
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