Experimental obeliscoidosis in rabbits. II. Immunohistological changes in the stomach and some aspects of immune response

• Autorzy: Zarnowska-Prymek H , Sinski E
• Języki publikacji: EN

Abstrakty

EN

The dynamics of IgA- and IgG-containing cells in the stomach mucosa of rabbits infected with Obeliscoides cuniculi was studied by means of a direct immunofluorescence method. An increase in the numbers of IgA-containing cells was found during the histotrophic phase of infection. An aggregation of secretory IgA on the external surface of the cuticle of worms was also observed. There was no stimulation of proliferation of local IgG-containing cells. The numbers of IgG-containing cells in infected rabbits were very low, and also very few IgG-containing cells were found in the stomach mucosa of uninfected rabbits. A weak immunological response in the blood sera, was observed only in a few animals after infection with various doses of larvae. No difference was found in the level of antibody titers as a result of exposure of animals to various doses of larvae. A significant increase in the number of IgA-containing cells in the stomach of infected rabbits might imply that О. cuniculi stimulated a local immune response of parasitized rabbits.

Słowa kluczowe

EN

obeliscoidosis; immunohistology; Obeliscoides cuniculi; stomach; rabbit; immune response

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1993
• Tom: 38
• Numer: 3
• Opis fizyczny: p.131-135,fig.

Twórcy

autor

Zarnowska-Prymek H

• Clinic of Zoonoses and Tropical Diseases, Institute of Infectious and Parasitic Diseases, Medical Academy, ul.Wolska 37, 01-201 Warszawa, Poland

autor

Sinski E

• Department of Parasitology, Zoological Institute, University of Warszawa, 00-927 Warszawa, Poland

Bibliografia

• Adams D. В., Beh K. J. 1981. Immunity acquired by sheep from an experimental infection with Haemonchus contortus. International Journal for Parasitology, 11, 381-386.
• Bozicevich J. 1938. Studies on trichinosis. XII. The preparation and use of an improved trichina antigen. Public Health Reports, 53, 2130-2138.
• Byszewska-Szpocińska E., Stankiewicz M. 1983. Immunological studies on experimental haemonchosis in sheep. Ш. Appearance of antibodies in sera and abomasal mucosae after single infection. Acta Parasitologica Polonica, 28, 481-488.
• Crandall R. B., Cebra J. J., Crandall C. A. 1967. The relative proportions of IgG, IgA and IgM-containing cells in rabbit tissues during experimental trichinosis. Immunology, 12, 147-158.
• Gill H. S., Husband A. J., Watson D. L. 1992. Localization of immunoglobulin-containing cells in the abomasum of sheep following infection with Haemonchus contortus. Veterinary Immunology and Immunopathology, 31, 179-187.
• Helal J. B., Wędrychowicz H., Siński E., Bezubik B. 1987. Inhibition phenomena mechanisms in experimental obeliscoidosis in rabbits. II. Local and systemic antibody responses. Journal of Helminthology, 61,115-123.
• Kessel J. F., Lewis W.P., Molina-Pasquel C., Turner J. A. 1965. Indirect hemagglutination and complement fixation tests in amebiasis. American Journal of Tropical Medicine and Hygiene, 14, 540-550.
• Krupp J. M. 1969. Glutaraldehyde treated cells in the indirect hemagglutination test for amebiases. American Journal of Tropical Medicine and Hygiene, 18, 666-669.
• Poulain J., Luffau G., Pery P. 1976. Nippostrongylus brasiliensis in the rat. Immune response in serum and intestinal secretions. Annales ďImmunologie, 127c, 215-224.
• Sęk S. 1978. Zastosowanie metod statystycznych w badaniach klinicznych i eksperymentalnych. Centrum Medyczne Kształcenia Podyplomowego, Warszawa.
• Silverman P. H., Patterson J. E. 1960. Histotrophic (parasitic) stages of Haemonchus contortus. Nature, London, 185, 54-55.
• Sinclair I. J., Wassall D. A., Cawthorme R. J. G. 1985. Demonstration of IgА-containing cells in the small intestine of lambs infected with Nematodirus battus. Research in Veterinary Science, 38, 373-376.
• Siński E. 1975. Immunological investigations in experimental ostertagiosis in sheep. III. Serological changes and analysis of antibodies in the sera and abomasal mucosa after single and multiple infections. Acta Parasitologica Polonica, 23, 637-652.
• Siński E. 1983. The role of local immunity in resistance to nematode infections. I. An investigation of IgA and IgM responses of gut associated lymphoid tissue (GALT) in rats infected with Nippostrongylus brasiliensis. Acta Parasitologica Polonica, 28, 327-335.
• Siński E., Bezubik B. 1980. Immunological investigations in experimental ostertagiosis in sheep. VI. Local immunoglobulin levels after single infection. Acta Parasitologica Polonica, 27, 399-407.
• Siński E., Holmes P. H. 1977. Nippostrongylus brasiliensis: systemic and local IgA and IgG immunoglobulin responses in parasitized rats. Experimental Parasitology, 43, 382-389.
• Siński E., Holmes P. H. 1978. In vitro binding of IgG and IgA to Nippostrongylus brasiliensis measured by radioimmunoassay. Journal of Parasitology, 64, 189-191.
• Siński E., MacLean J. M., Holmes P. H. 1984. The role of local immunity in resistance to nematode infections. III. The effects of sublethal gamma-irradiation of rats on the kinetics of systemic and gut associated lymphoid tissue (GALT) responses following infection with Nippostrongylus brasiliensis. Acta Parasitologica Polonica, 29, 265-275.
• Smith W. D., Christie M. G. 1978. Resistance to Haemonchus contortus: local and serum antibodies in sheep immunised with irradiated larvae. International Journal for Parasitology, 8, 219-223.
• Smith H. V., Herbert J. V., Davis A. J. 1979. The immune response of pigs to infection with the stomach worm Hyostrongylus rubidus (Hassal and Stiles, 1892). III. Effect of primary infection on immunoglobulin positive cell numbers. Immunology, 38, 659-664.
• Sollod A. E., Allen J. R. 1971. Immunity to Obeliscoides cuniculi: chemical suppression and passive transfer. Journal of Parasitology, 57, 339-342.
• Wędrychowicz H., Siński E., Bezubik B. 1988. Surface antigens of Obeliscoides cuniculi inducing local and systemic humoral responses in infected rabbits. Parasitology Research, 74, 386-392.
• Żamowska-Prymek H. 1991. Experimental obeliscoidosis in the rabbit. I. Anatomo- and histopathological changes in the wall of the stomach. Acta Parasitologica Polonica, 36, 145-151.