Opracowanie zestawu diagnostycznego do wykrywania przeciwcial anty-Hypoderma bovis u bydla testem ELISA. 3.Badanie trwalosci i przydatnosci komponentow do testu ELISA przechowywanych w roznych warunkach

• Autorzy: Cencek T , Ziomko I. , Karamon J.
• Języki publikacji: PL

Abstrakty

EN

The aim of investigations was to determine the stability of ELISA components preserved under different conditions. The following components for the ELISA were used: the secreted/excreted antigen prepared from H. bovis L1 larvae, its fraction containing hypodermine A, conjugat, positive and negative control sera. Freezing at -20°C, lyophilization and preservation at 4°C were applied. Seven kits for the ELISA containing components preserved using described methods, were prepared., The identical packets of sera were examined by the ELISA directly after preparation of the kits (day '0'), and next, at 3-4 week intervals for 9 months. The experiments showed that the best method of conservation of ELISA components for the detection of H. Bovis antibodies was lyophilization. The kits prepared in this way did not lose their characteristics for 9 months and their specificity and sensitivity were close 100%.

Słowa kluczowe

PL

komponenty; pasozyty zwierzat; bydlo; choroby pasozytnicze; Hypoderma bovis; giez bydlecy; gzawica; diagnostyka; warunki przechowywania; trwalosc; zestawy diagnostyczne; przeciwciala; parazytologia; przeciwciala anty-Hypoderma bovis; choroby zwierzat; przydatnosc; wykrywanie; test ELISA

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2001
• Tom: 47
• Numer: 3
• Opis fizyczny: s.511-520,rys.,tab.,bibliogr.

Twórcy

autor

Cencek T

• Panstwowy Instytut Weterynaryjny, ul.Partyzantow 57, 24-100 Pulawy

autor

Ziomko I.

autor

Karamon J.

Bibliografia

• BOULARD C. 1985. Avantages de l'immunodiagnostic de l'hypodermose bovine etabli par hemagglutination passive et par ELISA, a partir du serum et lactoserum, sur la numeration des varons. Ann. Rech. Vet. 16(4): 335-43.
• PRIETO M., ESPI A., GARCIA PALOMA J. A., PARRA F. 1990. Effect of ivermectin treatment on anti-hypodermin C titers of Asturiana cattle naturally infected with Hypoderma lineatum. Vet. Parasitol. 35(3): 211-218.
• SINCLAIR I. J., WASSALL D. A. 1983. Enzyme-linked immunosorbent assay for the detection of antibodies to Hypoderma bovis in cattle. Res. Vet. Sci. 34(2): 251-252.
• ZIOMKO I., CENCEK T. 1997. Effect of preservation conditions of Hypoderma bovis antigens on their activity in the ELISA. Bulletin of the Veterinary Institute in Puławy 41(1): 25-28.