Expression of ras-family genes in the cell cycle and during differentiation of the lower eukaryote Physarum polycephalum

• Autorzy: Fronk J.
• Języki publikacji: EN

Abstrakty

EN

Messenger RNA levels of three rcw-family genes (Pprasl, Ppras2, and Pprapl) were measured in different life forms and throughout the cell cycle of the slime mold Physarum polycephalum. All three genes are expressed at constant rates in the uni­nucleate amoebae and flagellates, regardless of the culture conditions (solid or liquid medium, particulate or dissolved nutrients). In the multinucleate stages (micro- and macroplasmodia) Pprasl and Pprapl mRNAs are somewhat less abundant, while Ppras2 is not expressed at all. The early stages of the amoeba-plasmodium transition proceed without any drop in Ppras2 expression. During the synchronous cell cycle in macroplasmodia Pprasl and Pprapl arc expressed at a constant level.

Słowa kluczowe

EN

Physarum polycephalum; cell cycle; ras-family gene; slime mould; eukaryote; RNA level; differentiation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 1
• Opis fizyczny: p.197-202,fig.

Twórcy

autor

Fronk J.

• Warsaw University, S.Zwirki i F.Wigury 93, 02-089 Warsaw, Poland

Bibliografia

• 1. Lacal, J.L. & McCormick, F. (eds.) (1993) The ras superfanily of GTPases. CRC Press, Boca Raton.
• 2. Olkkonen, V.M. & Stenmark, H. (1997) Role of Rab GTPases in membrane traffic. Int. Rev. Cytol 176, 1-85.
• 3. Machesky, L.M. & Hall, A. (1996) Rho: A con­nection between membrane receptor signal­ling and the cytoskeleton. Trends Cell Biol 6, 304-310.
• 4. Lowy, D.R. & WiUumsen. B.M. (1993) Func­tion and regulation of ras. Annu. Rev. Bio- chem. 62, 851-891.
• 5. Capon, D.J., Seeburg, P.H., McGrath, J.P., Hayflick, J.S., Edman, U., Levinson, A.D. & Goeddel, D.V. (1983) Activation of Ki-nw2 gene in human colon and lung carcinomas by two different point mutations. Nature 304, 507-513.
• 6. Avruch, J., Zhang, X.F. & Kyriakis, J.M. (1994) Ras meets Raf: Completing the frame­work of a signal transduction pathway. Trends Biochem. Set 19, 279-283.
• 7. Marshall, C.J. (1996) Ras effectors. Curr. Opin. Cell Biol 89, 197-204.
• 8. Vojtek, A.B. & Der, C.J. (1998) Increasing complexity of the Ras signaling pathway. J. Biol Chem. 273, 19925-19928.
• 9. Chang, H.C., Karim, F.D., O'Neill, E.M., Re- bay, I., Solomon, N.M., Therrien, M., Was- sarmn, D.A.. Wolff, T. & Rubin, G.M. (1994) Ras signal transduction pathway in Droso- phila eye development Cold Spring Harbor Symp. 59, 147-153.
• 10. Han, M. & Sternberg, P.W. (1990) let60, a gene that specifies cell fates during C. elegans vulval induction, encodes a ras protein. Cell 63, 921-931.
• 11. Broach, J.R. (1991) RAS genes in Saccharomy- ces cerevisiae: Signal transduction in search of a pathway. Trends Genet 7, 28-33.
• 12. Burland, T.G., Solnica-Krezel, L., Bailey, J., Cunningham, D.B. & Dove, W.F. (1993) Patr terns of inheritance, development and the mi-totic cycle in the protist Physarum polycepha­lum. Adv. Microbial Physiol. 35, 1-69.
• 13. Kozłowski, P., Fronk, J. & Toczko, K. (1993) Identification of a ras gene in the slime mold Physarum polycephalum. Biochim. Biophys. Acta 1173, 357-359.
• 14. Kozłowski, P. Tymowska, Z. & Toczko, K. (1993) Nucleotide and predicted amino acid sequence of a new member of the ras gene family from the slime mold Physarum poly­cephalum. Biochim Biophys. Acta 1174, 299- 302.
• 15. Kozłowski, P., Trzcinska-Danielewicz, J. & Toczko, K. (1996) Identification and sequence analysis of a rap gene from the true slime mold Physarum polycephalum. Biochim. Bio­phys. Acta 1305, 29-33.
• 16. Kozłowski, P., Trzcinska-Danielewicz, J., Ur­bańczyk, A. & Toczko, K. (1997) Pprasl, Ppras2 and Pprapl genes, members of a ras gene family from the true slime mold Physa­rum polycephalum, are developmental^ regu­lated. Cell Biol. Intern. 21, 441-445.
• 17. Aldrich, H.C. & Daniel, J.W. (eds.) (1982) Cell Biology of Physarum and Didymium. Aca­demic Press, New York.
• 18. Burland, T.G., Gull, K., Schedl, T., Boston, R.S. & Dove. W.F. (1983) Cell type-dependent expression of tubulins in Physarum. J. Cell Biol 97, 1852-1859.
• 19. Hamelin, M., Adam, L., Lemieux, M. & Pal- lotta, D. (1988) Expression of the three un­linked isocoding actin genes of Physarum polycephalum. DNA 7, 317-328.
• 20. Wilhelm, M.L. & Wilhelm, F.X. (1984) A transposon-like DNA fragment interrupts a Physarum polycephalum histone H4 gene. FEBS Lett 168, 249-254.
• 21. Wilhelm, M.L., Toublan, B., Jalouzot, R. & Wilhelm, F.X. (1984) Histone H4 gene is tran­scribed in S phase but also late in Gz phase in Physarum polycephalum. EMBO J. 3, 2659- 2662.
• 22. Daniel, J., Bush, J., Cardelli, J., Spiegelman, G.B. & Weeks, G. (1994) Isolation of two novel ras genes in Dictyostelium discoideum; Evi­dence for a complex, developmentally regu­lated ras gene subfamily. Oncogene 9, 501- 508.
• 23. Bailey, J. (1995) Plasmodium development in the myxomycete Physarum polycephalum: Ge­netic control and cellular events. Microbiology 141, 2355-2365.
• 24. Barbieri, M.A., Kohn, A.D., Roth, R.A. & Stahl, P.D. (1998) Protein kinase B/akt and Rab5 mediate ras activation of endocytosis. J. Biol Chem. 273, 19367-19370.