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1995 | 42 | 2 |

Tytuł artykułu

Expression analysis of Cucurbita cDNA encoding endonuclease

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The nuclear matrices of plant cell nuclei display intrinsic nuclease activity which consists in nicking supercoiled DNA. A cDNA encoding a 32 kDa endonuclease has been cloned and sequenced. The nucleotide and deduced amino-acid sequences show high homology to known 14-3-3 protein sequences from other sources. The amino-acid sequence shows agreement with consensus sequences for potential phosphorylation by protein kinase A and C and for calcium, lipid and membrane-binding sites. The nucleotide-binding site is also present within the conserved part of the sequence. By Northern blot analysis, the differential expression of the corresponding mRNA was detected; it was the strongest in sink tissues. The endonuclease activity found on DNA-polyacrylamide gel electrophoresis coincided with mRNA content and was the highest in tuber.

Wydawca

-

Rocznik

Tom

42

Numer

2

Opis fizyczny

p.183-189,fig.

Twórcy

autor
  • Wroclaw University, S.Przybyszewskiego 63, 51-148 Wroclaw, Poland

Bibliografia

  • 1. Rudnicki, R., Rzepecki, R. & Szopa, J. (1988) Rearrangement of the nuclease-inhibitor complex components within cells of white bush treated with phytohormone./. Plant Physiol. 132, 658-663.
  • 2. Rzepecki, R., Szmidzinski, R., Bode, J. & Szopa, J. (1992) The 65 kDa protein affected endonuclease tightly associated with plant nuclear matrix. }. Plant Physiol. 139, 284^288.
  • 3. Szopa, J. & Adamiec, R. (1993) Is the 65 kDa protein a direct signal for the nuclease release from nuclear matrix, starting the apoptotic cascade? Acta Biochim. Polon. 40,209-212.
  • 4. Szopa, J. & Rose, K.M. (1986) Cleavage of the 190 kDa subunit of RNA polymerase I yields small polypeptides capable of degrading DNA./. Biol. Chem. 261, 9022-9028.
  • 5. Furukawa, Y., Ikuta, N., Omata, S., Yamauchi, T., Isobe, T. & Ichimura, T. (1993) Demonstration of the phosphorylation-dependent interaction of tryptophan hydroxylase with the 14-3-3 protein. Biochem. Biophys. Res. Commun. 194, 144-149.
  • 6. Isobe, T., Hiyane, Y., Ichimura, T., Okuyama, T., Takahashi, N., Nakajo, S. & Nakaya, K. (1992) Activation of protein kinase C by the 14-3-3 proteins homologous with exol protein that stimulates calcium-dependent exocytosis. FEBS Lett. 308,121-124.
  • 7. de Vetten, N.C., Lu, G. & Ferl, R.J. (1992) A maize protein associated with the G-box binding complex has homology to brain regulatory proteins. Plant Cell 4,1295-1307.
  • 8. Pearson, W.R. & Lipman, D.J. (1988) Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. U.S.A. 85,2444-2448.
  • 9. Logemann, J., Schell, J. & Willmitzer, L. (1987) Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 163,16-20.
  • 10. Rogers, S.O. & Bendich, A.J. (1985) Extraction of DNA from miligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol. 5,69-76.
  • 11. Gonzalez, G.A., Yamamoto, K.K., Fischer, W.H., Karr, D., Menzel, P., Biggs III, W., Vale, W.W. & Montminy, M.R. (1989) A cluster of phospho­rylation sites on the cyclic AMP-regulated nuclear factor CREB predicted by its sequence. Nature (London) 337, 749-752.
  • 12. Lu, G., DeLisIe, A.J., de Vetten, N.C. & Ferl, R.J. (1992) Brain proteins in plants: An Arabidopsis homolog to neurotransmitter pathway activators is part of a DNA binding complex. Proc. Natl. Acad. Sci. U.S.A. 89,11490-11494.
  • 13. Aitken, A., Ellis, C.A., Sellers, LA. & Toker, A. (1990) Kinases and neurotransmitters. Nature (London) 344,594.
  • 14. Toker, A., Ellis, C.A., Sellers, L.A. & Aitken, A. (1990) Protein kinase C inhibitor proteins. Eur. ]. Biochem. 191,421^29.
  • 15. Leffers, H., Madsen, P., Rasmussen, H.H., Honore, B., Andersen, A.H., Walbum, E., Vandekerckhove, J. & Celis, J.E. (1993) Molecular cloning and expression of the transformation sensitive epithelial marker stratifin. A member of a protein family that has been involved in the protein kinase C signalling pathway. J. Mol. Biol. 231,982-998.
  • 16. Leung, H.B., Kvalnes-Krick, K.L., Meyer, S.L., deRiel, J.K. & Schramm, V.L. (1989) Structure and regulation of the AMP nucleosidase gene (amn) from Escherichia coli. Biochemistry 28, 8726-8733.
  • 17. Coffman, C., Harris, W. & Kintner, C. (1990) Xotch, the Xenopus homolog of Drosophila Notch. Science 249,1438-1441.
  • 18. Geryk, J., Dezelee, P., Barnier, J.V., Svoboda, J., Nehyba, J., Karakoz, I., Rynditch, A.V., Yatsula, B.A. & Calothy, G. (1989) Transduction of the cellular src gene 3' adjacent sequences in avian sarcoma virus PR2257. J. Virol. 63,481-492.
  • 19. Fu, H., Coburn, J. & Collier, R.J. (1993) The eucaryotic host factor that activates exoertzyme S of Pseudomonas aeruginosa is a member of the 14-3-3 protein family. Proc. Natl. Acad. Sci. U.S.A. 90, 2320-2324.
  • 20. Raikhel, N. (1992) Nuclear targeting in plants. Plant Physiol. 100,1627-1632.
  • 21. Hirsch, S., Aitken, A., Bertsch, U. & Soil, A. (1992) A plant homologue to mammalian brain 14-3-3 protein and kinase C inhibitor. FEBS Lett. 296, 222-224.
  • 22. Brandt, J., Thordal-Christensen, H., Vad, K., Gregersen, P.L. & Collinge, D.B. (1992) A pathogen-induced gene of barley encodes a protein showing high similarity to a protein kinase regulator. Plant J. 2,815-820.

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Bibliografia

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