Proces apoptozy w przebiegu doswiadczalnego zarazenia T. pseudospiralis u myszy

• Autorzy: Widyma A , Piekarska J.
• Języki publikacji: PL

Abstrakty

EN

The apoptosis in the course of experimental infection with T. pseudospiralis in mice. The level of apoptic cells in the lamina propria of the mucosa of the jejunum and in the masseter muscle in the mice infected with 200 larvae T. pseudospiralis was observed. Cryostat preparations made from jejunum and muscle samples on 7, 14, 21, 28, 60, 180 and 360 day post infection (dpi), were examined employing the TUNNEL method with the use of "In situ Cell Death Detection KIT Fluorescein" of Reche. The number of apoptic cells in the lamina propria of jejunum started to increase as early as on 7 dpi. The highest number of the apoptic cells, on an average - 56,4 in the crypta-villus unit, was recorded on 28 dpi, whereas the retum to the standard level took place only 360 dpi. The level of the apoptic cells in the masseter muscle was the highest oo 28 and 60 dpi, which coostituted some 30% of all the cells of infiltration.

Słowa kluczowe

PL

apoptoza; Trichinella pseudospiralis; zwierzeta doswiadczalne; parazytologia; myszy; pasozyty

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2002
• Tom: 48
• Numer: 4
• Opis fizyczny: s.413-417,bibliogr.

Twórcy

autor

Widyma A

• Instytut Parzytologii PAN, ul.Norwida 31, 50-375 Wroclaw

autor

Piekarska J.

Bibliografia

• Abou-Afifi M., Gamra M.M., Moustafa M.A, Hoseiny L.M. 2000. Modulation of the pathologic and apoptotic changes of experimental toxoplasmosis by concomitant infection with Trichinella spiralis. Journal of Egypt Society of Parasitology 30: 69-81.
• Carneiro-Santos P., Martins-Filho O., Alves-Oliveira L.F, Silveira A.M., Coura-Filho P., Viana I.R., Wilson R.A., Correa-Oliveira R. 2000. Apoptosis: a mechanism of immunoregulation during human schistosomiasis mansoni. Parasite Immunology 2: 267-277.
• Estaquier J., Marguesite M., Sahuc F., Bessis N., Auriault C., Ameisen J.C. 1997. Interleukin-10-mediated T cell apoptosis during the T helper type 2 cytokine response in murine Schistosoma mansoni parasite infection. European Cytokine Network 8: 153-160 (cyt. za Helmintologinal Abstract 1998. 67: 195).
• Fush D., Bajer-Bitterlich G., Wachter H. 1996. Impaired Th1-like immune response in Schistosoma mansoni infection. Journal of Immunology and Infection Diseases 174: 677-678.
• Garside P., Sands W.A., Kusel J.R., Hagan P. 1996. Is the induction of apoptosis the mechanism of the protective effects of TNFα in helminth infection? Parasite Immunology 18: 111-113.
• Gavrieli Y., Sherman Y., Bensesson S. 1992. Identilication of programmed cell death in silu via specific labeling of nuclear DNA fragmentation. Journal of Cell Biology 119: 493-501.
• Hyoh Y., Nishida M., Yamada M., Uchikawa R., Matsuda S., Arizono N. 1999. Enhancement of apoptosis with lass of cellular adherence in the villus epithelium of the small intestine after infection with the nematode Nipposirongylus brasiliensis in rats. Parasitology 119: 199-207.
• Jung S.K., Mai A., Iwamoto M., Arizono N., Fujimoto D., Sakamaki K., Yonehara S. 2000.· Purification and cloning of an apoptosis - inducing protein derived from fish infected with Anisakis simplex, a causative nematode of human anisakiasis. Journal of Immunology 165: 1491-1497.
• Karmańska K., Houszka M., Piekarska J. 2000. The phenomenon of apoptosis in the course of experimental trichinellosis in mice. Wiadomości Parazytologiczne 46: 111-115.
• Kozar Z., Kozar M. 1971. Diagnostyka chorób pasożytniczych człowieka. PZWL. Warszawa.
• Lane Petter W., Pearson A. 1971. The labolatory animal - principles and practice. Academic Press, London-New York.
• Nash P.B., Purner M.B., Leon R.P., Clarke P., Duke R.C., Curiel T.J. 1998. Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis. Journal of Immunology 160: 1823-1830.
• O'Connell K.M., Rogan M.T. 2000. Apoptosis in human Jurkat T cell after culture with live Taenia crassiceps cysticerci in vitro. Parasitology 120: 649-655.
• Piekarska J., Widyma A.2000. Apoptosis of cells isolated from mause of lymphatic node induced by ES antigen of T. spiralis. Electronic Journal of Polish Agricultural Universities (w druku).
• Shin M.H. 2000. Excretory-secretory product of newly excysted metacercariae of Paragonimus westermani directly induces eosinophil apoptosis. Korean Journal of Parasitology 38: 17-23.
• Starke W., Oaks J. 1999. Hymenolepis diminuta: praziquantel removal of adult tapeworms is followed by apoptotic down - regulation of mucosal mastocytosis. Experimental Parasitology 92: 171-181.