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2006 | 62 | 02 |

Tytuł artykułu

DNA Anaplasma phagocytophilum we krwi saren oraz w pozyskanych z nich kleszczach

Autorzy

Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
The aim of the study was to establish the role of roe deer (Capreolus capreolus) as reservoirs of Anaplasma phagocytophilum and the role of Ixodes ricinus ticks as vectors of this pathogen in NorthWest Poland. Blood samples of 127 roe deer were collected between May and June 2004 and between September and December 2004. 51 of the 63 roe deer caught between May and June 2004 and 7 of the 64 roe deer caught between September and December 2004 were infested by I. ricinus ticks. 170 individuals of the common tick were collected from 45.7% of roe deer. PCR amplification of a fragment of the msp2 gene was used for detecting A. phagocytophilum DNA. Pathogen DNA was detected in 30 of the blood samples (23.6%) and in 10 ticks collected from the animals (5.9%). 28.6% of the roe deer caught between May and June 2004 and 18.75% of the roe deer caught between September and December 2004 were infected, but this difference was not significant. 5.3% of I. racinus ticks were infected by A. phagocytophilum. It is clear that C. capreolus is a significant reservoir of A. phagocytophilum in NorthWest Poland. In addition, I. ricinus is a significant vector in this area.

Wydawca

-

Rocznik

Tom

62

Numer

02

Opis fizyczny

s.201-203,tab.,bibliogr.

Twórcy

autor
  • Uniwersytet Szczecinski, al. Piastow 40B, 71-065 Szczecin

Bibliografia

  • 1.Alberdi M. P., Walker A. R., Urquhart K. A.: Field evidence that roe deer (Capreolus capreolus) are a natural host for Ehrlichia phagocytophila. Epidemiol. Infect. 2000, 124, 315-323.
  • 2.Alekseev A. N., Dubinina H. V., van de Pol I., Schouls L. M.: Identification of Ehrlichia spp. and Borrelia burgdorferi in Ixodes ticks in the Baltic regions of Russia. J. Clin. Microbiol. 2001, 39, 2237-2242.
  • 3.Bakken J. S., Dumler J. S.: Human granulocytic ehrlichiosis. Clin. Infect. Dis. 2000, 31, 554-560.
  • 4.Bjöersdorff A., Bergstrom S., Massung R. F., Haeming P. D., Olsen B.: Ehrlichia-infected ticks on migrating birds. Emerg. Infect. Dis. 2001, 7, 877-879.
  • 5.Blanco J. R., Oteo J. A.: Human granulocytic ehrlichiosis in Europe. Clin. Microbiol. Infect. 2002, 8, 763-772.
  • 6.Bown K. J., Begon M., Bennett M., Woldehlwet Z., Ogden N. H.: Seasonal dynamics of Anaplasma phagocytophila in rodent-tick (Ixodes trianguliceps) system, United Kingdom. Emerg. Infect. Dis. 2003, 9, 63-70.
  • 7.Comer J. A., Nicholson W. I., Sumner J. W., Olson J. G., Childs J. E.: Diagnosis of human ehrlichiosis by PCR assay acute-phase serum. J. Clin. Microbiol. 1999, 37, 31-34.
  • 8.Daniels T. J., Battaly G. R., Liveris D., Falco R. C., Schwartz I.: Avian reservoirs of the agent of human granulocytic ehrlichiosis? Emerg. Infect. Dis. 2002, 8, 1524-1525.
  • 9.Guy E. C., Stanek G.: Detection of Borrelia burgdorferi in patient with Lyme disease by the polymerase chain reaction. J. Clin. Pathol. 1991, 44, 610-611.
  • 10.Hulinska D., Votypka J., Plch J., Vlcek E., Valesova M., Bojar M., Hulinsky V., Smetana K.: Molecular and microscopial evidence of Ehrlichia spp. and Borrelia burgdorferi sensu lato in patients, animals and ticks in the Czech Republic. New Microbiol. 2002, 5, 437-448.
  • 11.Levin M. L., des Vignes F., Fish D.: Disparity in the natural cycles of Borrelia burgdorferi and the agent of human granulocytic ehrlichiosis. Emerg. Infect. Dis. 1999, 5, 204-208.
  • 12.Liz J. S., Anderes L., Sumner J. W., Massung R. F., Gern L., Rutti B., Brossard M.: PCR detection of granulocytic Ehrlichiae in Ixodes ricinus ticks and wild small mammals in Western Switzerland. J. Clin. Microbiol. 2000, 38, 1002-1007.
  • 13.Massung R. F., Slater K.: Comparision of PCR assays for detection of the agent of human granulocytic ehrlichiosis, Anaplasma phagocytophilum. J. Clin. Microbiol. 2003, 41, 717-722.
  • 14.Mikulski T.: Statystyka medyczna. PAM, Szczecin 1994, 94-95.
  • 15.Petrovec M., Bidovec A., Sumner J. W., Nicholson W. L., Childs J. E., Avsinc- -Zupanc T.: Infection with Anaplasma phagocytophila in cervids from Slovenia: evidence of two genotypic lineages. Wien. Klin. Wochenschr. 2002, 114, 641-647.
  • 16.Skotarczak B., Rymaszewska A.: Wstępne badania czynnika etiologicznego ludzkiej ehrlichiozy (HGE) w kleszczach z zachodniopółnocnej Polski. Wiad. Parazytol. 2001, 47, 95-101.
  • 17.Skotarczak B., Rymaszewska A., Sawczuk M., Adamska M., Suproñ M., Jerszyñska-Mazur J.: PCR detection of granulocytic Anaplasma and Babesia in Ixodes ricinus ticks and yellow-necked mice (Apodemus flavicollis) in West-Central Poland. A Parazytol, in press.
  • 18.Skotarczak B., Rymaszewska A., Wodecka B., Sawczuk M., Adamska M., Suproñ M., Maciejewska A.: PCR detection of granulocytic Anaplasma and Babesia in Ixodes ricinus ticks and birds in West-Central Poland. Ann. Agric. Environ. Med, in press.
  • 19.Stañczak J., Racewicz M., Kruminis-£ozowska W., Kubica-Biernat B.: Coinfection of Ixodes ricinus (Acari: Ixodidae) in northern Poland with the agents of Lyme borreliosis (LB) and human granulocytic ehrliosis (HGE). Int. J. Med. Microbiol. 2002, 33, 198-201.
  • 20.Stuen S., Akerstedt J. K., Bergström K., Handeland K.: Antibodies to granulocytic Ehrlichia in moose, red deer, and roe deer in Norway. J. Wildl. Dis. 2002, 38, 1-6.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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