Metabolism of cyclic GMP in peritoneal macrophages of rat and guinea pig

• Autorzy: Kobialka M. , Witwicka H. , Siednienko J. , Gorczyca W.A.
• Języki publikacji: EN

Abstrakty

EN

The aim of our studies was to establish which enzymes constitute the "cGMP pathway" in rat and guinea pig peritoneal macrophages (PM). We found that in guinea pig PM synthesis of the nucleotide was significantly enhanced in response to activators of soluble guanylyl cyclase (sGC) and it was only slightly stimulated by specific activa­tors of particulate guanylyl cyclases (pGC). In contrast, rat PM responded strongly to atrial natriuretic peptide (ANP), the activator of pGC type A. The rat cells synthesized about three-fold more cGMP than an equal number of the guinea pig cells. The activity of phosphodiesterases (PDE) hydrolyzing cGMP was apparently regulated by cGMP itself in PM of both species and again it was higher in the rat cells than in those isolated from guinea pig. However, guinea pig PM revealed an activity of Ca2+ /cal- modulin-dependent PDE1, which was absent in the rat cells. Using Western blotting analysis we were unable to detect the presence of cGMP-dependent protein kinase 1 (PKG1) in PM isolated from either species. In summary, our findings indicate that particulate GC-A is the main active form of GC in the rat PM, while in guinea pig macrophages the sGC activity dominates. Since the profiles of the PDE activities in rat and guinea pig PM are also different, we conclude that the mechanisms regulating cGMP metabolism in PM are species-specific. Moreover, our results suggest that tar­gets for cGMP other than PKG1 should be present in PM of both species.

Słowa kluczowe

EN

phosphodiesterase; guanylyl cyclase; macrophage; signal transduction; cyclic nucleotide; guinea pig; peritoneal macrophage; rat; protein kinase

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 3
• Opis fizyczny: p.837-847,fig.

Twórcy

autor

Kobialka M.

• Polish Academy of Sciences, R.Weigla 12, 53-114 Wroclaw, Poland

autor

Witwicka H.

autor

Siednienko J.

autor

Gorczyca W.A.

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