Microsatellite markers discriminating accessions within collections of plant genetic resources

• Autorzy: Kraic J , Gregova E. , Jomova K. , Hudcovicova M.
• Języki publikacji: EN

Abstrakty

EN

The reliability of microsatellite analyses for discriminating between plant accessions maintained in collections of genetic resources was tested for 53 accessions of barley, 65 of soybean, 49 of chickpea, and 19 of alfalfa. The specific primer pairs used in this study were based on microsatellite DNA sequences surrounded by perfect dinucleotide and imperfect trinucleotide tandem repeat units. The evaluated polymorphic information content, diversity index, and probabilities of identity indicate that there is value in the application of SSR analyses in barley, soybean, and chickpea genetic resource management. Variation between alfalfa genotypes was not revealed at the five analyzed microsatellite loci.

Słowa kluczowe

EN

microsatellite marker; alfalfa; chick-pea; soybean; genotype; barley; plant genetics; discrimination

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2B
• Opis fizyczny: p.745-751,fig.

Twórcy

autor

Kraic J

• Research Institute of Plant Production, Bratislavska cesta 122, 92168 Piest'any, Slovakia

autor

Gregova E.

autor

Jomova K.

autor

Hudcovicova M.

Bibliografia

• 1. Powell, W., Machray, G. C. and Provan, J. Polymorphism revealed by simple sequence repeats. Trends Plant. Sci. 1 (1996) 215-222.
• 2. Coryell, V. H., Jensen, H., Schupp, J. M., Webb, D. and Keim P. Allele-specific hybridization markers for soybean. Theor. Appl. Genet. 98 (1999) 690-696.
• 3. Liu, W., Biyashev, R. M. and Saghai Maroof, M. A. Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor. Appl. Genet. 93 (1996) 869-876.
• 4. Macaulay, M., Ramsay, L., Powell, W. and Waugh R. A representative, highly informative “genotyping set” of barley SSRs. Theor. Appl. Genet. 102 (2001) 801-809.
• 5. Dellaporta, S. L., Wood, J. and Hicks, J. B. A plant DNA minipreparation: Version II. Plant Mol. Biol Rep. 4 (1993) 19-21.
• 6. Rongwen, J., Ahhaya, M. S., Bhagwat, A. A., Lawi, U. and Cregan, P. B. The use of microsatellite DNA markers for soybean genotype identification. Theor. Appl. Genet. 90 (1995) 43-48.
• 7. Winter, P., Benko-Iseppon, A. M., Huttel, B., Ratnaparkhe, M., Tullu, A., Sonnante, G., Pfaff, T., Tekeoglu, M., Santra, D., Rajesh, P. N., Kahl, G. and Muehlbauer, F. J. A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum x C. reticulatum cross: localization of resistance genes for fusarium wilt races 4 and 5. Theor. Appl. Genet. 101 (2000) 1155-1163.
• 8. Diwan, N., Bhagwat, A. A., Bauchan, G. B. and Cregan, P. B. Simple sequence repeat DNA markers in alfalfa and perennial and annual Medicago species. Genome 40 (1997) 887-895.
• 9. Bassam, B. J., Caetano-Anolles, G. and Gresshoff, P. M. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196 (1991) 80-83.
• 10. Weir, B. S. Genetic data analysis. II. Methods for discrete population genetic data. 2nd ed. Sinauer Associates, Sunderland, Mass.
• 11. Paetkau, D., Calvert, W., Stirling, I. and Strobeck, C. Microsatellite analysis of population structure in Canadian polar bear. Mol. Ecol. 4 (1995) 347-354.
• 12. Weber, J. L. Informativeness of human (dC-dA)n . (dG-dT)n polymorphism. Genomics 7 (1990) 524-530.
• 13. Kraic, J., Žáková, M. and Gregová, E. Comparison of differentiation capability of RAPD and SSR markers in commercial barley (Hordeum vulgare L.) cultivars. Cereal Res. Commun. 26 (1998) 375-382.