Immunoaffinity purification of juvenile hormone-binding protein from Galleria mellonella hemolymph

• Autorzy: Wieczorek E. , Rodriguez-Parkitna J.M. , Szkudlarek J. , Ozyhar A. , Kochman M.
• Języki publikacji: EN

Abstrakty

EN

Previously described methods of purification of hemolymph juvenile hormone- binding protein (hJHBP) from Lepidoptera were tedious and required multiple steps. These methods resulted in low protein yield (Kramer if al., 1976; Goodman et al., 1978; Peterson et al., 1982; Park et al., 1993; Ożyhar & Kochman, 1987). In this report a simple method of purification of hJHBP from Galleria mellonella (L.) larvae is described. Monoclonal antibodies against hJHBP were obtained and crosslinked to CNBr- -activated Sepharose 4B. The hemolymph of G. mellonella was centrifuged and then chromatographed on Sephadex G-200 gel filtration column. Juvenile-hormone- -binding activity containing material from Sephadex G-200 column was subjected to purification on an immunoaffinity column. Bound protein was eluted from anti-hJHBP Sepharose 4B gel by lowering pH to 3.0 with 200 mM citric acid 200 mM Na2HP04 buffer. This method resulted in 320-fold purification of G. mellonella hJHBP with 56% yield.

Słowa kluczowe

EN

Lepidoptera; Galleria mellonella; monoclonal antibody; immunoaffinity purification; juvenile hormone; binding protein; hemolymph; antibody

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1996
• Tom: 43
• Numer: 4
• Opis fizyczny: p.603-610,fig.

Twórcy

autor

Wieczorek E.

• Technical University of Wroclaw, Wybrzeze S.Wyspianskiego 27, 50-370 Wroclaw, Poland

autor

Rodriguez-Parkitna J.M.

autor

Szkudlarek J.

autor

Ozyhar A.

autor

Kochman M.

Bibliografia

• BhuIIar, R.P. & Seneviratne, H.D. (1996) Charac­terization of human platelet GTPase-activating factor protein for the RALGTP-binding protein. Biochim. Biophys. Acta 1311,181-188.
• Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72,248-254.
• Chiao, J.H., Yang, C.H., Roy, K., Pain, J. & Sirotnak, F.M. (1995) Ligand-directed immunoaffinity purification properties of the one-carbon, re­duced folate transporter. J. Biol. Chem. 270, 29698-29704.
• Cymborowski, B. (1984) Lndokrynologia owaddw. PWN (in Polish).
• DippoId, W.G., Lloyd, K.O., Li, L.T.C., Ikeda, H., Oettgen, H.F. & Old, I..J. (1980) Cell surface antigens of human malignant melanoma: De­finition of six antigenic systems with mouse monoclonal antibodies. Proc. Natl. Acad. Sci. U.S.A. 77, 6114-6118.
• Dunbar, B.S. & Schwoebel, E.D. (1990) Preparation of polyclonal antibodies. Methods Ettzymol. 182 663-670.
• Gingras, D., White, D., Garin, J., Multigner, L., Job, D., Cosson, J., Huitorel, P., Zingg, H., Dumas, F. & Gagnon, C. (1996) Purification, cloning and sequence analysis of a Mr = 30000 protein from sea urchin axoncmes that is important for sperm mobility. /. Biol. Chem. 271,12807-12813.
• Goodman, W., a Hern, P.A., Zaugg, R.H. & Gilbert, 1. (1978) Purification and characterization of a juvenile hormone binding protein from the hemolymph of the fourth instar tobacco horn- worm, Manduca sexta. Molec. Cell. Lndocrin. 11, 225-242.
• Hidayat, P. & Goodman, W.G. (1994) Juvenile hor­mone and hemolymph juvenile hormone bind­ing protein liters and their interaction in the hemolymph of fourth stadium Manduca sexta. Insect Biochem. Molec. Biol. 24, 709-715.
• Jones, C., Patel, A., Griffin, S., Martin, J., Young, P., O'Donnell, K., Silverman, A., Porter, T. & Chaiken, I. (1995) Current trends in molecular recognition and bioseparation. J. Chromatogr. A 707, 3-22.
• Kochman, M. & Wieczorek, E. (1991) Molecular mechanism of the juvenile hormone action. Acta Biochim. Polon. 38,393-405.
• Kochman, M. & Wieczorek, E. (1995) Proteins in­volved in juvenile hormone signal transmission; in Insects. Chemical, Physiological and Environ­mental Aspects. 1994. (Konopińska, D., Gold- sworthy,G.,Nachman, R.J., Nawrot, J., Orchard, I., Rosiński, G. & Sobótka, W., eds.) pp. 92-118, Wydawnictwo Uniwersytetu Wrocławskiego, Wrocław, Poland.
• Kohler, G. & Milstein, C. (1975) Continuous cultures of fused cells sccrcting antibody of predefined specificity. Nature (Ijondon) 256,495-497.
• Kramer, K.J., Dunn, PH., Peterson, R.C., Seballos, H.L., Sanburg, L.L. & Law, J.H. (1976) Purifica­tion and characterization of the carrier protein for juvenile hormone from the haemolymph of the tobacco homworm Manduca sexta Johann- son (l^epidoptera: Sphingidae). /. Biol. Chem. 251,4979-4985.
• Kwatra, M.M., Schreurs, J., Schwinn, D.A., Innis, M.A., Caron, M.G. & Lefkowitz, R.J. (1995) 1m- munoaffinity purification of epitope-tagged human beta(2)-adrenergic receptor to homo­geneity. Protein Expression & Purification 6, 717- 721.
• Laemmli, U.K. (1970) Cleavage of structural pro­teins during assembly of the head of bacterioph­age T4. Nature (London) 227, 680-685.
• Merril, C.R. (1990) Gel-staining techniques. Methods Lnzymol. 182,477-488.
• Ożyhar, A., Wiśniewski, J., Sehnal, F. & Kochman, M. (1983) Age dependent changes in the binding and hydrolysis of juvenile hormone in the hemolymph of Gatleria mellonella. Insect Bio- chem. 13,435-441.
• Ożyhar, A. & Kochman, M. (1986) Generation of multiple forms of juvenile hormone binding protein from Galleria mellonella hemolymph. Ex- perientia 42,1276-1278.
• Ożyhar, A. & Kochman, M. (1987) Juvenile-hor­mone-binding protein from the hemolymph of Galleria mellonella (L). Isolation and characteri­zation. Eur. J. Biochem. 162, 675-682.
• Park, Y.Ch., Tesch, M.J., Toong, Y.C. & Goodman, W.G. (1993) Affinity purification and binding analysis of the hemolymph juvenile hormone binding protein from Mmuiuca sexta. Biochem­istry 32,7909-7915.
• Peterson, R.C., Dunn, P.E., Seballos, H.L, Barbeau, B.K., Kcim, P.S., Riley, C.T., Heinrikson, R.L. & I .aw, J.H. (1982) Juvenile hormone carrier pro­tein of Manduca sexta haemolymph. Improved purification procedure: Protein modification studies and sequence of the amino terminus of the protein. Insect Biochem. 12,643-650.
• Pharmacia (1977) Affinity chromatography. Princi­ples and methods.
• Riddiford, L.M. (1994) Cellular and molecular ac­tions of juvenile hormone I. General consider­ations and premetamorphic actions. Adv. Insect Physiol. 24, 213-271.
• Sanburg, L., Kramer, K., Kezdy, F., Law, J. & Ober­länder, H. (1975) Role of juvenile hormone ester­ases and carrier proteins in insect development. Nature (London) 253,266-267.
• Sehnal, F. & Slama, K. (1966) The effect of corpus allatum hormone on respiratory metabolism during larval development and metamorphosis of Galleria mellonella. J. Insect Physiol. 12, 1333- 1342.
• Seya, T., Matsumoto, M., Hatanaka, M., Okada, M., * Masaki, T. & lida, K. (1996) Rapid purification of human-complement receptor-type-1 (Cd35, Cr-1). J. Biochem. Biophys. Methods 32, 69-76.
• Trautmann, K.H., Schuler, A., Suchy, M. & Wipf, K.H. (1974) Method for qualitative and quanti­tative determination of three natural insect juvenile hormones. Evidence of methyl 10,11- epoxy-3,7,11 trimethyl-2-frans, 6-/rans-
• Vannier, B., Loosfelt, H., Meduri, G., Pichon, C. & Milgrom, E. (1996) Anti-human FSH receptor monoclonal antibodies: immunochemical and immunocytochemical characterization of the re­ceptor. Biochemistry 35,1358-1366.
• Wieczorek, E. (1994) Ph.D. Thesis. Politechnika Wro­cławska.
• Wiśniewski, J.R., Muszyńska-Pytel, M., Grzelak, K. & Kochman, M. (1987) Biosynthesis and degra­dation of juvenile hormone in corpora allata and imaginal wing discs of Galleria mellonella (L.). Insect. Biochem. 17,249-254.