Spontaneous calcium oscillations in urinary bladder smooth muscle cells

• Autorzy: Martin-Cano F.E. , Gomez-Pinilla P.J. , Pozo M.J. , Camello P.J.
• Języki publikacji: EN

Abstrakty

EN

Although spontaneous phasic activity of detrusor muscle plays an important role in urinary bladder function there is little information regarding myogenic [Ca2+]i signals in this tissue. We have studied spontaneous, unstimulated [Ca2+]i signals in fura-2 loaded detrusor cells isolated from newborn (10-13 days old) guinea-pig urinary bladder. In newborn guinea pigs 35% of studied muscle cells displayed spontaneous [Ca2+]i oscillations with several kinetic patterns (from irregular to highly paced cycles). The oscillations were inhibited by external Ca2+ removal, treatment with L- and T-type Ca2+ channel blockers and by the hyperpolarizing drug pinacidil. Ca2+ stores were necessary to maintain oscillations, as indicated by the inhibitory effects of thapsigargin, ryanodine and 2-APB. Oscillations were also inhibited by folimycin, an inhibitor of acidic Ca2+ stores. Treatment with the selective inhibitors iberiotoxin and NPPB indicated that the oscillatory signal is also modulated by Ca2+ -activated K+ channels (inhibitory) and Ca2+ -activated Cl- channels (stimulatory). Our results indicate that detrusor cells from newborn guinea-pigs develop spontaneous [Ca2+]i oscillations due to Ca2+ influx through T- and L-type Ca2+ channels modulated by intracellular stores, including acidic pools. This activity could underlie the myogenic activity of urinary bladder during early stages of development.

Słowa kluczowe

EN

urinary bladder function; smooth muscle; cell; detrusor muscle; myogenic calcium signal; tissue; calcium channel; guinea pig

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2009
• Tom: 60
• Numer: 4
• Opis fizyczny: p.93-99,fig.,ref.

Twórcy

autor

Martin-Cano F.E.

• University of Extremadura, 10071 Caceres, Spain

autor

Gomez-Pinilla P.J.

autor

Pozo M.J.

autor

Camello P.J.