Thermal profile with alternately raised and lowered annealing temperature improves the PCR amplification using highly degenerate primers

• Autorzy: Sachadyn P. , Sobiewska G. , Kur J.
• Języki publikacji: EN

Abstrakty

EN

The proposed here PCR thermal profile improves the specificity and efficiency of PCR using highly degenerate primers, especially in the case of larger PCR products (around 2000 bp and more). The improvement is achieved by the use of a specific annealing temperature in the beginning cycles and the alternate lowering and raising of the annealing temperature in the subsequent cycles.

Słowa kluczowe

EN

temperature; thermal profile; glucosamine-6-phosphate synthase; polymerase chain reaction; degenerate primer

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 3
• Opis fizyczny: p.691-694,fig.

Twórcy

autor

Sachadyn P.

• Technical University of Gdansk, G.Narutowicza 11-12, 80-952 Gdansk, Poland

autor

Sobiewska G.

autor

Kur J.

Bibliografia

• 1. von Eggeling, F. & Spielvogel, H. (1995) Appli­cations of random PCR. Cell Mol. Biol. 41, 653-670.
• 2. Wesley, C.S., Ben, M., Kreitman, M., Hagag, N. & Eanes, W.F. (1989) Cloning regions of the DrosophUa genome by microdissection of polytene chromosome DNA and PCR with non-specific primer. Nucleic Acids Res. 18, 599- 603.