Seasonal patterns of free proline and carbohydrate levels in cherry laurel (Prunus laurocerasus) and ivy (Hederea helix) leaves and resistance to freezing and water deficit

• Autorzy: Bandurska H , Plachta M. , Woszczyk M.
• Języki publikacji: EN

Abstrakty

EN

Seasonal changes in the levels of water-soluble carbohydrates, free proline and tolerance to freezing and water deficit were studied in leaves of cherry laurel (Prunus laurocerasus) and ivy (Hedera helix). Frost and water deficit tolerance was estimated based on the measurement of electrical conductivity of aqueous media containing leaf discs that were previously treated with frost (–7°C) or polyethylene glycol solution (PEG 600), respectively. Carbohydrate content in leaves of examined species changed differently throughout the measurement period. In laurel leaves the highest carbohydrate level was found in February, while it was lowest in June. In contrast, in ivy leaves the highest carbohydrate content was recorded during summer (June, July), while the lowest in February. However, a lack of correlation between soluble carbohydrate level and membrane injury index was shown in this study. Free proline content in leaves of investigated species was the highest in early spring, i.e. April. The lowest level of free proline in laurel leaves was found in July, September and October, but in ivy from July to February. A negative correlation between proline content and membrane injury index observed in frost and PEG treated leaves of both species suggest that this amino acid may have resulted on membrane protection.

Słowa kluczowe

EN

evergreen shrub; woody plant; seasonal change; temperature; climate; water-soluble carbohydrate; proline; free proline; carbohydrate level; leaf; cherry laurel; Prunus laurocerasus; ivy; Hedera helix; freezing resistance; water deficit; resistance

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2009
• Tom: 62
• Opis fizyczny: p.3-9,fig.,ref.

Twórcy

autor

Bandurska H

• Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland

autor

Plachta M.

autor

Woszczyk M.

Bibliografia

• Ashraf M., Foolad M.R. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59: 206–216.
• Aniśko T. 2002. Cold hardiness of evergreen cherries. American Nurseryman 195(6): 42–48.
• Bandurska H. 2000. Does proline accumulated in leaves of water deficit stressed barley plants confine cell membrane injury? I. Free proline accumulation and membrane injury index in drought and osmotically stressed plants. Acta Physiologiae Plantarum 22: 409–415.
• Bates L.S., Waldren R.P., Teare J.D. 1973. Rapid determination of proline for water stress studies. Plant and Soil 39: 205–207.
• Beck E.H., Heim R., Hansen J. 2004. Plant resistance to cold stress: Mechanisms and environmental signals triggering frost hardening and dehardening. Journal of Biosciences 29: 449–459.
• Björnesjö K.B. 1955. Analysis of protein-bound serum polysaccharides with anthrone reagent. Scandinavian Journal of Clinical and Laboratory Investigation 7: 147–152.
• Diamantoglou S., Rhizopoulou S. 1992. Free proline accumulation in sapwood, bark and leaves of three evergreen sclerophylls and comparison with an evergreen conifer. Journal of Plant Physiology 140: 361–365.
• Frankow-Lindberg B.E. 2001. Adaptation to winter stress in nine white clover populations: Changes in non-structural carbohydrates during exposure to simulated winter conditions and spring re-growth potential. Annals of Botany 88: 745–751.
• Hare P.D., Cress W.A., Van Staden J. 1998. Dissecting the roles of osmolyte accumulation during stress. Plant Cell and Environment 21: 535–553.
• Hurry V.M., Strand Å. Tobićson M., Gardeström P., Öquist G. 1995. Cold hardening of spring and winter wheat and rape results in differential effects on growth, carbon metabolism, and carbohydrate content. Plant Physiology 109: 697–706.
• Karolewski P., Pukacki P.M. 1995. Free proline as an indicator of sensitivity of Norway spruce (Picea abies) to low temperature. Arboretum Kórnickie 40: 159–167.
• Kishor P.B.K., Sangam S., Amrutha R.N., Laxmi P.S., Naidu K.R., Rao K.R.S.S., Rao S., Reddy K.J., Theriappan P., Sreenivasulu N. 2005. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plants growth and abiotic stress tolerance. Current Science 88: 424–438.
• Keskitalo J., Bergquist G., Gardeström P., Jansson S. 2005. A cellular timetable of autumn senescence. Plant Physiology 139: 1635–1648.
• Larcher W. 2005. Climatic constraints drive the evolution of low temperature resistance in woody plants. Journal of Agricultural Meteorology 61: 189–202.
• Mahajan S., Tuteja N. 2005. Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics 444: 139–158.
• Molinari H.B.C., Marur C.J., Bespalhok J.C., Kobayashi A.K., Pileggi M., Leite R.P., Pereira L.F.P., Vieira L.G.E. 2004. Osmotic adjustment in transgenic citrus rootstock Carrizo citrange (Citrus sinensis OSB. X Poncirus trifoliata L. Raf.) overproducing proline. Plant Science 167: 1375–1381.
• Moussa H.R., Abel-Azis S.M. 2008. Comparative response of drought tolerant and drought sensitive maize genotypes to water stress. Australian Journal of Crop Science 1: 31–36.
• Miyazawa Y., Kikuzawa K. 2005. Winter photosynthesis by saplings of evergreen broad leaved trees in deciduous temperate forest. New Phytologist 165: 857–866.
• Nanjo T., Kobayashi M., Yoshiba Y., Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K. 1999. Antisense suppression of proline degradation improves tolerance to freezing and salinity in Arabidopsis thaliana. FEBS Letters 461: 205–210.
• Öztürk L., Demir Y. 2002. I vivo and in vitro protective role of proline. Plant Growth Regulation 38: 259–264.
• Oleksyn J., Zytkowiak P., Karolewski P., Reich P.B., Tjoelker M.G. 2000. Genetic and environmental control of seasonal carbohydrate dynamics in trees of diverse Pinus sylvestris populations. Tree Physiology 20: 837–847.
• Patton A.J., Cunningham S.M., Volenec J.J., Reicher Z.J. 2007. Differences in freeze tolerance of zoysiagrasses: II. Carbohydrate and proline accumulation. Crop Science 47: 2170–2181.
• Premachandra G.S., Saneoka H., Fujita K., Ogata S. 1993. Seasonal changes in leaf water relations and cell membrane stability in Orchardgrass (Dactylis glomerata). Journal of Agricultural Science 121: 169–175.
• Premachandra G.S., Shimada T. 1987. Measurement of drought tolerance in orchardgrass (Dactylis glomerata L.). I. Polyethylene glycol test of measuring cell membrane stability. Journal of Japanese Society of Grassland Science 33: 140–148.
• Pukacki P.M. 1995. Membrane properties of Norway spruce cell during long-term freezing stress. Arboretum Kórnickie 40: 125–133.
• Qureshi M.I., Qaidr S., Zolla L. 2007 Proteomic-based dissection of stress-responsive pathways in plants. Journal of Plant Physiology 164: 1239–1260.
• Sauter J.J. 1988. Temperature-induced changes in starch and sugars in steam of Populus × cana canadiensis “Robusta”. Journal of Plant Physiology 132: 608–612.
• Shahba M.A., Qian Y.L., Hughes H.G., Koski A.J., Christensen D. 2003. Relationships of soluble carbohydrates and freeze tolerance in saltgrass. Crop Science 43: 2148–2153.
• Smallwood M., Bowles D.J. 2002. Plants in a cold climate. Philosophical Transactions of Royal Society (B) 357: 831–847.
• Steponkus P.L., Uemura M., Webb M.S. 1993. Membrane destabilization during freeze-induced dehydration. Current Topics in Plant Physiology 10: 37–47.
• Sullivan C.Y. 1971. Techniques for measuring plant drought stress. In: Drought injury and resistance in crops. Larson K.I., Eastin J.D. (eds.). Crop Science Society of America, Madison, Wisconsin, pp. 1–18.
• Stitt M., Hurry V. 2002. A plant of all seasons: alteration in photosynthetic carbon metabolism during cold acclimation in Arabidopsis. Current Opinion in Plant Biology 5: 199–206.
• Swaaij A.C., Jacobsen E., Feenstra W.J. 1985. Effect of cold hardening, wilting and exogenously applied proline on leaf proline content and frost tolerance of several genotypes of Solanum. Physiologia Plantarum 64: 230–236.
• Swearingen J.M., Dietrich S. 2006. English ivy. http://www.invasive.org/weedcd/pdfs/wgw/englishivy.pdf
• Thomashow M.F. 1999. Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms. Annual Review of Plant Physiology and Plant Molecular Biology 50: 571–599.
• Webb M.S., Steponkus P.L. 1993. Freeze-induced membrane ultrastructural alterations in Rye (Secale cereale) leaves. Plant Physiology 101: 955–963.
• Xin Z., Browse J. 2000. Cold comfort farm: The acclimation of plant to freezing temperatures. Plant Cell and Environment 23: 893–902.