Diagnosis of Brucella suis infections in pigs and hares by ELISA

• Autorzy: Szulowski K.
• Języki publikacji: EN

Abstrakty

EN

Two ELISA kits for diagnosis of Brucella suis infections in pigs and hares were evaluated. The evaluation was performed by comparison of the results obtained in the ELISA with those in traditional serological methods. To evaluate the ELISA for testing pig sera, 100 samples from pigs from infected herds, 40 samples from pigs from doubtful herds and 18 672 sera from healthy pigs monitored for brucellosis were involved. As concerns the ELISA for testing hare sera, samples from 16 animals suspected of Brucella infection (in this number 12 hares revealed clinical or anatomopathological lesions characteristic of brucellosis), 8 samples from healthy hares and 1 120 samples from hares monitored for anti-Brucella antibodies were involved. The ELISA has proved to be more specific than the RBT when testing pig sera and more sensitive in testing both pig and hare sera. A high correlation between the ELISA and CFT was found. All 97 pig sera positive or doubtful in the CFT reacted positively in the ELISA, whereas only 1 serum out of 99 positive in the ELISA was negative in the CFT. Out of 53 sera hare sera positive in the ELISA, 51 revealed the anti-Brucella antibodies in the CFT (the remaining 2 were not tested). The ELISA proved to be a valuable and appropriate method for testing both pigs and hares for Brucella suis infections.

Słowa kluczowe

EN

pig; serology; Brucella suis; diagnosis; ELISA test; hare

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 1999
• Tom: 02
• Numer: 2
• Opis fizyczny: p.65-70,fig.

Twórcy

autor

Szulowski K.

• National Veterinary Research Institute, Pulawy, Poland