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2007 | 48 | 4 |

Tytuł artykułu

The use of the Vibrio harveyi luminescence mutagenicity assay as rapid test for preliminary assessment of mutagenic pollution of marine sediments

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Mutagenic pollution of the natural environment is currently one of the most serious environmental problems. It includes the pollution of marine sediments. Therefore, rapid detection of the presence of mutagens is an important issue. Recently, we have developed a novel microbiological assay for rapid assessment of mutagenicity of samples from the natural environment. This assay is based on bioluminescence of a mutant Vibrio harveyi strain, and was shown to be useful in testing samples of marine water and plant tissues. Here we demonstrate the usefulness of this assay in preliminary assessment of mutagenic pollution of marine sediments. Mutagenicity of environmental samples taken from the Baltic Sea, is documented and compared here with a commercially available standard sediment sample (IAEA 383), which contains known amounts of mutagenic compounds. The whole procedure, from obtaining a sample in the laboratory to getting final results, is very short (less than 4 h).

Słowa kluczowe

Wydawca

-

Rocznik

Tom

48

Numer

4

Opis fizyczny

p.409-412,fig.,ref.

Twórcy

autor
  • Institute of Oceanology, Polish Academy of Sciences, Sopot, Poland
autor
  • Institute of Oceanology, Polish Academy of Sciences, Sopot, Poland
autor
  • Institute of Oceanology, Polish Academy of Sciences, Sopot, Poland
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland

Bibliografia

  • Au WW, Oberheitmann B, Heo MY, Hoffmann W, Oh HY, 2001. Biomarker monitoring for health risk based on sensitivity to environmental mutagens. Rev Environ Health 16: 41-64.
  • Barton HA, Cogliano VJ, Flowers L, Valcovic L, Setzer RW, Woodruff TJ, 2005. Assessing susceptibility from early-life exposure to carcinogens. Environ Health Perspect 113: 1125-1133.
  • Chęć E, Podgórska B, Węgrzyn G, 2006. Direct addition of cultures of tester bacteria into semipermeable membrane devices (SPMDs) as a modified procedure for preliminary detection of mutagenic pollution of marine environment using microbiological mutagenicity assays. Mutat Res 611: 17-24.
  • Chęć E, Podgórska B, Węgrzyn G, 2007. Comparison of the use of mussels and semipermeable membrane devices for monitoring and assessment of accumulation of mutagenic pollutants in marine environment in combination with a novel microbiological mutagenicity assay. Environ Monit Assess (in press) DOI 10.1007/s 10661 -007-9849-1.
  • Chen G, White PA, 2004. The mutagenic hazards of aquatic sediments: a review 567: 151-225.
  • Czyż A, Plata K, Węgrzyn G, 2002. Induction of light emission by luminescent bacteria treated with UV light and chemical mutagens. J Appl Genet 43: 377-389.
  • Depledge MH, 1998. The ecotoxicological significance of genotoxicity in marine invertebrates. Mutat Res 399: 109-122.
  • El-Bayoumy K, 1992. Environmental carcinogens that may be involved in human breast cancer etiology. Chem Res Toxicol 5: 585-590.
  • Goldman R, Shields PG, 2003. Food mutagens. J Nutr 133: 965-973.
  • Heddle JA, Moody J, Thompson LU, Torous DK, Trentin G, 1999. New approaches to antimutagenesis. J Environ Pathol Toxicol Oncol 18: 95-101.
  • Jha AN, 2004. Genotoxicological studies in aquatic organisms: an overview. Mutat Res 552: 1-17.
  • Klein G, Walczak R, Krasowska E, Błaszczak A, Lipińska B, 1995. Characterization of heat-shock response of the marine bacterium Vibrio harveyi. Mol Microbiol 16: 801-811.
  • Maron DM, Ames BN, 1983. Revised methods for the Salmonella mutagenicity test. Mutat Res 113: 173-215.
  • Martin FL, 2001. Genotoxins and the initiation of sporadic breast cancer. Mutagenesis 16: 155-161.
  • Meighen EA, 1988. Enzymes and genes from the lux operons of bioluminescent bacteria. Annu Rev Microbiol 42: 151-176.
  • Podgórska B, Węgrzyn G, 2006. A modified Vibrio harveyi mutagenicity assay based on bioluminescence induction. Lett Appl Microbiol 42: 578-582.
  • Podgórska B, Pazdro K, Pempkowiak J, Węgrzyn G, 2007a. The use of a novel Vibrio harveyi luminescence mutagenicity assay in testing marine water for the presence of mutagenic pollution. Mar Pollut Bull 54: 808-814.
  • Podgórska B, Królicka A, Łojkowska E, Węgrzyn G, 2007b. Rapid detection of mutagens accumulated in plant tissues using a novel Vibrio harveyi mutagenicity assay. Ecotoxicol Environ Saf (in press) DOI 10.1016/j.ecoenv.2007.04.004.
  • Tornqvist M, Ehrenberg L, 2001. Estimation of cancer risk caused by environmental chemicals based on in vivo dose measurement. J Environ Pathol Toxicol Oncol 20: 263-271.
  • Vargas VM, 2003. Mutagenic activity as a parameter to assess ambient air quality for protection of the environment and human health. Mutat Res 544: 313-319.
  • Węgrzyn G, Czyż A, 2003. Detection of mutagenic pollution of natural environment using microbiological assays. J Appl Microbiol 95: 1175-1181.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-57d19a20-c64e-4467-8f4f-6e8ffad2f665
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