Gene segregation in a barley DH population

• Autorzy: Szarejko I , Maluszynski M , Polok K , Zbieszczyk J
• Języki publikacji: EN

Abstrakty

EN

A large population of anther culture-derived barley regenerants and their progeny was tested for allele segregation at 1 isozyme and 8 morphological marker loci. The segregation of genetic markers was examined separately for haploid, diploid and polyploid regenerants. All the 9 analysed genes except al (albino lemma) on chromosome 3 segregated according to the expected 1:1 ratio in the microspore-derived barley population. There was no difference in allele distribution between haploid and diploid regenerants. Among the limited number of 34 analysed tetraploids a significant excess of the dominant allele at locus о (orange lemma) of chromosome 6 was also observed. The recombination frequency between linked genes (n - lk2 on chromosome 1 and r - s on chromosome 7) estimated in the DH population did not differ significantly from recombination rates calculated in F₂ progeny or presented in barley chromosome maps. The phenomenon of gametic selection is discussed in relation to the genotype dependency of anther culture response and procedures used for DH production in barley.

Słowa kluczowe

EN

doubled haploid; barley; haploid plant; gametoclonal variation; gametic selection; anther culture; population; gene segregation

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 4
• Opis fizyczny: p.415-424

Twórcy

autor

Szarejko I

• Department of Genetics, Silesian University, Jagiellonska 28, 40-032 Katowice, Poland

autor

Maluszynski M

• Joint FAO/IAEA Division, Plant Breeding and Genetics Section, IAEA, 1400-Vienna, Austria

autor

Polok K

• Department of Genetics, Silesian University, Jagiellonska 28, 40-032 Katowice, Poland

autor

Zbieszczyk J

• Department of Genetics, Silesian University, Jagiellonska 28, 40-032 Katowice, Poland

Bibliografia

• Cai Q., Szarejko I., Polok K., Maluszynski M. (1992). The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture. Plant Breeding 109: 218-226.
• Chen C.C., Kasha K.J., Marsolais A. (1984). Segmentation patterns and mechanisms of genome multiplication in cultured microspores of barley. Can. J. Genet. Cytol. 26: 475-483.
• Foroughi-Wehr B., Friedt W. (1984). Rapid production of recombinant barley yellow mosaic virus resistant Hordeum vulgare lines by anther culture. Thcor. Appl. Genet. 67: 377-382.
• Heun M., Kennedy A.E., Anderson J.A., Lapatin N.L.V., Sorrells M.E., Tanksley S.D. (1991). Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34: 437-447.
• Immer F.R. (1930). Formulae and tables for calculating linkage intensities. Genetics 15: 81-98.
• Jensen J. (1981). Construction of barley chromosome 7 linkage map. In: Barley Gnetics IV, Edinburgh: 927-939.
• Kao K.N. (1988). In vitro culture in barley breeding. In: Semi-dwarf Cereal Mutants and Their Use in Cross-Breeding III, IAEA-TECDOC 455, Vienna: 183-191.
• Kasha K.J., Ziauddin A., Cho U.H. (1990). Haploids in cereal improvement: anther and microspore culture. In: Gene Manipulation in Plant Improvement II. Proc. 19th Stadler Genetics Symp (J.P. Gustafson, ed.). Plenum Press, New York: 213-235.
• Lee F.M., Chen C.C. (1987). Nuclear fusion in cultured microspores of barley. Plant Cell Rep. 6: 191-193.
• Liu J., Procunier J.D., Kasha K.J. (1991). Analysis of randomness of doubled haploid plants produced from anther culture and the Bulbosum method in barley (Hordeum vulgare L.). In: Barley Genetics VI. Vol. I (L. Munck, ed.). Helsingborg: 370-371.
• Maluszynski M. (1990). Induced mutations an integrating tool in genetics and plant breeding. In: Gene Manipulation in Plant Improvement II. Proc. 19th Stadler Genetics Symp. (J.P. Gustafson, ed.). Plenum Press, New York: 127-162.
• Pickering R.A., Devaux P. (1992). Haploid production: approaches and use in plant breeding. In: Barley: Genetics, Biochemistry, Molecular Biology and Biotechnology (P.R. Shcwry, ed.). CAB International, Wallingford: 519-547.
• Powell W., Borrino E.M., Allison M.J., Griffiths D.W., Asher M.J.C., Dunwell J.M. (1986). Gcnctical analysis of microspore derived plants of barley (Hordeum vulgare). Theor. Appl. Genet. 72: 619-626.
• Sall T. (1990). Genetic control of recombination in barley. II. Variation in linkage between marker genes. Hereditas 112: 171-178.
• Schon C., Sanchez M., Blake T., Hayes P.M. (1990). Segregation of Mendelian factors in doubled haploid and F₂ progeny of a barley cross. Hereditas 113: 69-72.
• Shaw C.R., Prasad R. (1970). Starch gel electrophoresis of enzymes - a compilation of recipes. Biochcm. Genet. 4: 297-320.
• Shin J.S., Chao S., Corpuz L., Blake T. (1990). A partial map of a barley genome incorporating restriction fragment length polymorphism, polymerase chain reaction, isozyme and morphological marker loci. Genome 33: 803-810.
• Snape J.W., Simpson E., Parker B.B., Friedt W., Foroughi-Wehr B. (1986). Criteria for the selection and use of doubled haploid systems in cereal breeding programmes. In: Genetic Manipulation in Plant Breeding (W. Horn, S.J. Jensen, W. Odenbach, О. Shieder, eds.). Walter de Gruyter, Berlin: 217-229.
• Szarejko I., Maluszynski M., Polok K., Kilian A. (1991). Doubled haploids in the mutation breeding of selected crops. In: Plant Mutat. Breeding Crop Improv. Vol. 2, IAEA, Vienna: 355-378.
• Szarejko I., Kasha K.J. (1991). Induction of anther culture derived doubled haploids in barley. Cereal Res. Commun. 19: 219-237.
• Thompson D.M., Chalmers K., Waugh R., Forster B.P., Thomas W.T.B., Caligari P.D.S., Powell W. (1991). The inheritance of genetic markers in microscope-derived plants of barley Hordeum vulgare L. Theor. Appl. Genet. 81: 487-492.
• Wettstein-Knowles von P. (1990). Barley (Hordeum vulgare) 2n=14. In: Genetic Maps. Locus Maps of Complex Genomes. Book 6. Plants (S.J. O’Brien, ed.). Cold Spring Harbour Lab. Press, Cold Spring Harbour: 6.125-6.134.