Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in Brazil

• Autorzy: Garcia-Melo D P , Regidor-Cerrillo J. , Ortega-Mora L.M. , Collantes-Fernandez E. , Ferreira de Oliveira V.S. , Pelli de Oliveira M.A. , Caetano da Silva A.
• Języki publikacji: EN

Abstrakty

EN

Neospora caninum is a tissue-cyst forming parasite that has been recognized worldwide as a cause of abortion in cattle. Despite the ubiquitous distribution of this parasite and its broad range of hosts, the number of N. caninum isolates obtained to date is limited. In addition, the majority of isolates have been obtained from clinically affected hosts, therefore potentially biasing this population towards more virulent isolates. This report describes the isolation and biological characterisation of a new N. caninum isolate, Nc-Goiás 1, obtained from an asymptomatic, naturally infected calf from Brazil. This new isolate was identified as a member of the N. caninum species by polymerase chain reaction (PCR) using specific primers based on the N. caninum internal transcribed spacer 1 (ITS-1) sequence, and was genetically identified at multiple loci using microsatellite analysis. Finally, a pathogenicity study was conducted in a BALB/c mice model. All Nc-Goiás 1-infected mice survived without exhibiting any clinical signs. Further pathogenic characterisation of this isolate suggested that Nc-Goiás 1 is less virulent than other N. caninum isolates (Nc-Liv and Nc-1) studied in this mouse model. This is the first report of the isolation and biological characterisation of N. caninum from an infected but clinically healthy calf in South America.

Słowa kluczowe

EN

parasite; cattle; Neospora caninum; neuromuscular disease; neonatal mortality; Brazil; animal disease; coccidian parasite; abortion; dog

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2009
• Tom: 54
• Numer: 2
• Opis fizyczny: p.180-185,fig.,ref.

Twórcy

autor

Garcia-Melo D P

• Universidade Federal de Goias, Caixa Postal 131-74001-970, Goiania, Goias, Brazil

autor

Regidor-Cerrillo J.

autor

Ortega-Mora L.M.

autor

Collantes-Fernandez E.

autor

Ferreira de Oliveira V.S.

autor

Pelli de Oliveira M.A.

autor

Caetano da Silva A.

Bibliografia

• Álvarez-García G., Pereira-Bueno J., Gómez-Bautista M., Ortega-Mora L.M. 2002. Pattern of recognition of Neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses. Veterinary Parasitology, 107, 15–27. DOI: 10.1016/S0304-4017(02)00091-2.
• Atkinson R., Harper P.A., Ryce C., Morrison D.A., Ellis J.T. 1999. Comparison of the biological characteristics of two isolates of Neospora caninum. Parasitology, 118, 363–370. DOI: 10.1017/S0031182098003898.
• Buxton D., Maley S.W., Wright S., Thomson K.M., Rae A.G., Innes E.A 1998. The pathogenesis of experimental neosporosis in pregnant sheep. Journal of Comparative Pathology, 118, 267–279. DOI: 10.1016/S0021-9975(07)80003-X.
• Collantes-Fernández E., López-Pérez I., Álvarez-García G., Ortega-Mora L.M. 2006a. Temporal distribution and parasite load kinetics in blood and tissues during Neospora caninum infection in mice. Infection and Immunity, 74, 2491–2494. DOI: 10.1128/IAI.74.4.2491-2494.
• Collantes-Fernandez E., Rodriguez-Bertos A., Arnaiz-Seco I., Moreno B., Aduriz G., Ortega-Mora L.M. 2006b. Influence of the stage of pregnancy on Neospora caninum distribution, parasite loads and lesions in aborted bovine foetuses. Theriogenology, 65, 629–641. DOI: 10.1016/j.theriogenology.2005.06.003.
• Collantes-Fernández E., Zaballos A., Alvarez-García G., Ortega-Mora L.M. 2002. Quantitative detection of Neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time PCR. Journal of Clinical Microbiology, 40, 1194–1198. DOI: 10.1128/JCM.40.4.1194-1198.
• Dubey J.P., Buxton D., Wouda W. 2006. Pathogenesis of bovine neosporosis. Journal of Comparative Pathology, 134, 267–289. DOI: 10.1016/j.jcpa.2005.11.004.
• Dubey J.P., Schares G., Ortega-Mora L.M 2007. Epidemiology and control of neosporosis and Neospora caninum. Clinical Microbiology Reviews, 20, 323–367. DOI: 10.1128/CMR.00031-06.
• Gondim L.F.P., Pinheiro A.M., Santos P.O.M., Jesus E.E.V., Ribeiro M.B., Fernandes H.S., Almeida M.A.O., Freire S.M., Meyer R., McAllister M.M. 2001. Isolation of Neospora caninum from the brain of a naturally infected dog, and production of encysted bradyzoites in gerbils. Veterinary Parasitology, 101, 1–7. DOI: 10.1016/S0304-4017(01)00493-9.
• Innes E.A., Wright S., Bartley P., Maley S., Macaldowie C., Esteban-Redondo I., Buxton D. 2005. The host-parasite relationship in bovine neosporosis. Veterinary Immunology and Immunopathology, 108, 29–36. DOI:10.1016/j.vetimm.2005.07.004.
• Locatelli-Dittrich R., Richartz R.R., Joineau M.E., Pinckney R.D., de Sousa R.S., Leite L.C., Thomaz-Soccol V. 2003. Isolation of Neospora caninum from a blind calf in Paraná, southern Brazil. Veterinary Records, 153, 366–367.
• Locatelli-Dittrich R., Thomaz-Soccol V., Richartz R.R.T.B., Gasino-Joineau M.E., Vander Vinne R., Pinckney R.D. 2004. Isolamento de Neospora caninum de feto bovino de rebanho leiteiro no Paraná (Isolation of Neospora caninum from bovine fetus from a dairy herd in Paraná). Revista Brasileira de Parasitologia Veterinária (Brazilian Journal of Veterinary Parasitology), 3, 103–109.
• Miller C.M., Quinn H.E., Windsor P.A., Ellis J.T. 2002. Characterisation of the first Australian isolate of Neospora caninum from cattle. Australian Veterinary Journal, 80, 620–625. DOI: 10.1111/j.1751-0813.2002.tb10967.x.
• Pedraza-Díaz S., Marugán-Hernández V., Collantes-Fernández E., Regidor-Cerrillo J., Rojo-Montejo S., Gómez-Bautista M., Ortega-Mora L.M. 2008. Genetic characterisation of Neospora caninum isolates from aborted bovine foetuses. In: 4th Annual Workshop I, COST Action 857, 4–7 May, 2007, Porticcio, France, 52.
• Pérez-Zaballos F.J., Ortega-Mora L.M., Álvarez-García G., Collantes-Fernández E., Navarro-Lozano V., García-Villada L., Costas E. 2005. Adaptation of Neospora caninum isolates to cell-culture changes: an argument in favor of its clonal population structure. Journal of Parasitology, 91, 507–510. DOI: 10.1645/GE-381R1.
• Regidor-Cerrillo J., Gómez-Bautista M., Pereira-Bueno J., Aduriz G., Navarro-Lozano V., Risco-Castillo V., Fernández-García A., Pedraza-Diaz S., Ortega-Mora L.M. 2008. Isolation and genetic characterization of Neospora caninum from asymptomatic calves in Spain. Parasitology, 135, 1651–1659. DOI: 10.1017/S003118200800509X.
• Regidor-Cerrillo J., Pedraza-Díaz S., Gómez-Bautista M., Ortega-Mora L.M. 2006. Multilocus microsatellite analysis reveals extensive genetic diversity in Neospora caninum. Journal of Parasitology, 92, 517–524. DOI: 10.1645/GE-713R.1.
• Rodrigues A.A., Gennari S.M., Aguiar D.M., Sreekumar C., Hill D.E., Miska K.B., Vianna M.C., Dubey J.P. 2004. Shedding of Neospora caninum oocysts by dogs fed tissues from naturally infected water buffaloes (Bubalus bubalis) from Brazil. Veterinary Parasitology, 124, 139–150. DOI: 10.1016/j.vetpar.2004.07.007.
• Rojo-Montejo S., Collantes-Fernández E., Regidor-Cerrillo J., Álvarez García G., Marugan-Hernández V., Pedraza-Díaz S., Blanco-Murcia J., Prenafeta A., Ortega-Mora L.M. 2009. Isolation and characterization of a low virulence bovine isolate of Neospora caninum. Veterinary Parasitology, 159, 7–16. DOI: 10.1016/j.vetpar.2008.10.009.
• Schock A., Innes E.A., Yamane I., Latham S.M., Wastling J.M. 2001. Genetic and biological diversity among isolates of Neospora caninum. Parasitology, 123, 13–23.
• Shibahara T., Kokuho T., Eto M., Haritani M., Hamaoka T., Shimura K., Nakamura K., Yokomizo Y., Yamane I. 1999. Pathological and immunological findings of athymic nude and congenic wild type BALB/c mice experimentally infected with Neospora caninum. Veterinary Pathology, 36, 321–327. DOI: 10.1354/vp.36-4-321.
• Trees A.J., Williams D.J. 2005. Endogenous and exogenous transplacental infection in Neospora caninum and Toxoplasma gondii. Trends in Parasitology, 21, 558–561. DOI: 10.1016/j.pt.2005.09.005.