Serological detection and variability of tomato yellow leaf curl virus isolates from Tanzania

• Autorzy: Kashina B D , Mabagala R.B. , Mpunami A.A.

Warianty tytułu

PL

Wlasciwosci transmisyjne wirusa zoltej kedzierzawosci pomidora z Tanzanii

• Języki publikacji: EN

Abstrakty

EN

Tomato farms in Arusha, Morogoro, Dodoma, Iringa, Kilimanjaro and Coast regions of Tanzania were surveyed to assess the incidence of the yellow leaf curl disease, and to collect infected tomato leaf samples for sero-diagnosis. The triple antibody sandwich enzyme linked immunosorbent assay (TAS-ELISA) format was adopted for the detection of disease using commercial polyclonal antiserum and monoclonal antibodies SCRI 17, SCRI 20, SCRI 23 and SCRI 33. ELISA readings were rated on a scale of 0-4. The results of the tests indicated that all the Tomato yellow leaf curl virus (TY- LCV) isolates recorded high reaction values (4) with the polyclonal antibody. However, the Dodoma and Arusha isolates were rated highest in optical density (OD) reading with MAb SCRI 20 and 23. The remaining isolates produced lower OD values. All the isolates rated low (2) when tested with SCRI 33. The differences in reaction to the monoclonal antibodies of TYLCV indicated that variability exists between the coat protein epitopes of TYLCV and Tomato yellow leaf curl Tanzania virus (TYL-CTZV) on one hand, and among the TYLCTZV isolates on the other. Only the isolates from Arusha and Dodoma share a high sequence homology in coat protein with the European and related TYLCV isolates. Furthermore, the reaction with either SCRI 20 or SCRI 23 show that the isolates from Arusha and Dodoma share a high degree of homology, and could belong to one serotype. The other isolates from Morogoro, Coast and Kilimanjaro could form another serotype, while the isolate from Iringa is a different serotype. On the other hand, reaction with SCRI 17 groups the isolates in two serotypes, the Dodoma isolate alone, and another that groups the other five isolates together. It is recommended that other procedures such as DNA-DNA hybridization assays, polymerase chain reaction, restriction fragment length polymorphisms and sequencing can be combined with the use of monoclonal antisera for the detection and prediction or inference of Tomato yellow leaf curl disease (TYLCD) virus relationships at the quasi-species or strain levels in Tanzania.

PL

Stwierdzono, że wirus żółtej kędzierzawości pomidora infekujący rośliny pomidora w Tanzanii różni się od geminiwirusów Starego Świata. Badania podjęto w celu określenia właściwości transmisyjnych tego wirusa takich jak czas żerowania wektora na roślinie porażonej potrzebny do nabycia przez niego wirusa, czas żerowania wektora na roślinie infekowanej potrzebny do jej inokulacji, czas życia wirusa w wektorze, inokulacja mechaniczna oraz przenoszenie przez nasiona lub przeszczepianie. Otrzymane wyniki wykazały, że wirus jest przenoszony przez Bemisia tabaci Genn., nie jest natomiast przenoszony mechanicznie ani przez nasiona. Minimalne czasy nabycia wirusa przez wektora i inokulacji rośliny wynosiły 30 minut. Zatem właściwości czynnika powodującego objawy żółtej kędzierzawości na pomidorach z różnych regionów Tanzanii są podobne i nie odbiegają od właściwości garunków Begomowirus badanych gdzie indziej.

Słowa kluczowe

EN

plant protection; serological detection; variability; tomato yellow leaf curl virus; isolation; Tanzania; tomato plant; plant farm; plant disease; monoclonal antibody

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2007
• Tom: 47
• Numer: 4
• Opis fizyczny: p.367-373,ref.

Twórcy

autor

Kashina B D

• Sokoine University of Agriculture, Tanzania

autor

Mabagala R.B.

autor

Mpunami A.A.

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