Transfer of divercin through ultrafiltration membranes after treatment with detergents and organic solvents

• Autorzy: Sip A. , Grajek W.
• Języki publikacji: EN

Abstrakty

EN

The ultrafiltration of post-culture fluids proved that divercin occurred in the form of large macroaggregates with the molecular weight over 100 kDa. Addition of detergents: Tween 80, Nonidet P-40 and SDS, and alcohols: ethanol and isopropanol into supernatants from post-culture fluids caused partial disaggregation of divercin. The better effect was obtained when Tween 80 was introduced directly into culture medium. However, other detergents and both alcohols showed a toxic effect to the bacteria and reduced the divercin production in C. divergens culture. The ultrafiltration yield of divercin depended on the type of membranes and treatment of divercin-containing extracts with detergents and alcohols. The cellulose acetate membranes were more permeable for divercin that made from polyethersulfone.

Słowa kluczowe

EN

polyethersulphone membrane; bacteriocin; detergent; alcohol; divercin; ultrafiltration; organic solvent; cellulose acetate membrane; aggregation; ultrafiltration membrane; Carnobacterium divergens

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology
• Rocznik: 2001
• Tom: 04
• Numer: 2
• Opis fizyczny: http://www.ejpau.media.pl

Twórcy

autor

Sip A.

• Agricultural University of Poznan, Wojska Polskiego 48, 60-627 Poznan, Poland

autor

Grajek W.

Bibliografia

• Delves-Broughton J. ,1990, Nisin and its application as a food preservative. J. Soc. Dairy Technol. 43: 73-76.
• Fitzgerald G.F., Vaughan E.V., Daly C., 1992, Identification and characterization of helveticin V-1829, a bacteriocin produced by Lactobacillus helveticus 1829. J. Appl. Bacteriol. 73: 299-308.
• Hansen J.N., 1994, Nisin as a model food preservative. Crit. Rev. Food Sci. Nutr. 34: 69-93.
• Joerger M. C., Klaenhammer T. R., 1986, Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481, J. Bacteriol. 167: 439-446.
• Kabuki T., Saito T., Kawai Y., Uemura J., Itoh T., 1997, Production, purification and characterization of reutericin 6, a bacteriocin with lytic activity produced by Lactobacillus reuteri LA6. Int. J. Food Microbiol. 34: 145-156.
• Khouiti Z., Simon J.P., 1997, Detection and partial characterization of bacteriocin produced by Carnobacterium piscicola 213. J. Ind. Microbiol. Biotechnol. 19: 28-33.
• Lyon W. J., Glatz B. A., 1993: Isolation and purification of propionicyn PLG-1, a bacteriocin produced by a strain of Propionibacter thoenii, Appl. Environ. Microbiol. 59: 83-88.
• Metivier A., Pilet M. A., Dousset X., Sorokine O., Anglade P., Zagorec M., Piard J. Ch., Marion D., Cenatiempo Y., Fremaux Ch., 1998, Divercin V41, a new bacteriocin with two disulphide bonds produced by Carnobacterium divergens V41: primary structure and genomic organization. Microbiology 144: 2837-2844.
• Mortvedt C. J., Nissen-Mayer J., Sletten K., Nes I. F., 1991, Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45. Appl. Environ. Microbiol. 57: 114-121.
• Muriana P. M., Klaenhammer T. R., 1991, Purification and partial characterization of lactocin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl. Environ. Microbiol. 57: 114-121.
• Pilet M. F., 1994, Characterisation de la divercine V41 et de la piscicocine V1, bacteriocines produites par Carnobacterium divergens V 41 et Carnobacterium piscicola V1 isolees de produits marins. Ph. Thesis, Universite de Caen, Institut de Recherches en Biologie Appliquee, pp 1-139.
• Pilet M. F., Dusset X., Barre R., Novel G., Desmazaeud M., Piard J. Ch., 1995, Evidence for two bacteriocins produced by Carnobacterium piscicola and Carnobacterium divergens isolated from fish and active against Listeria monocytogenes. J. Food Protect. 58: 256-262.
• Schved F., Lalazar A. Henis Y., Juven B. J., 1993, Purification, partial characterization and plasmid-linkage of pediocin SJ-1, a bacteriocin produced by Pediococcus acidilactici. J. Appl. Bacteriol. 74: 67-77.
• Sip A., Grajek W., 1997, Production of divercin by Carnobacterium divergens in presence of divercin-sensitive bacteria Carnobacterium piscicola and Listeria innocua. Pol. J. Food Nutr. Sci. 3: 1-6.