Activity of beta-glucosidase, arylsulfatase and phosphatases in soil contaminated with copper

• Autorzy: Wyszkowska J , Kucharski M. , Kucharski J.

Warianty tytułu

PL

Aktywnosc beta-glukozydazy, arylosulfatazy i fosfataz w glebach zanieczyszczonych miedzia

• Języki publikacji: EN

Abstrakty

EN

A pot experiment was carried out to determine the effect of soil (loamy sand and sandy loam) contamination with copper doses of 0, 150, 450 mg Cu·kg-1 d.m. soil on the activity of β-glucosidase (EC 3.2.1.21), acid phosphatase (EC 3.1.3.2), alkaline phosphatase (EC 3.1.3.1) and arylsulfatase (EC 3.1.6.1) in soil. The resistance of these enzymes to copper pollution was also estimated. Soil samples were contaminated with copper chloride. The experiment was carried out in five replications, in two series. The first series was performed on uncropped soil and the second one — on cropped soil. The experimental plants were oat, spring rape and yellow lupine. The activity of soil enzymes was determined in the analyzed samples on the 25th and the 50th day of the experiment. The results of the experiment showed that copper contamination in doses of 150 mg to 450 mg·kg-1 soil significantly inhibits soil’s biochemical activity. The sensitivity of the tested enzymes to copper was determined in the following order: alkaline phosphatase > arylsulfatase > acid phosphatase > β-glucosidase. The resistance of the above enzymes to copper depended on the cultivated plant spe- cies, soil type and the type of soil use and management. In samples of sandy loam, copper induced the smallest change in the activity of acid phosphatase and alkaline phosphatase, and in loamy sand — in the activity of arylsulfatase and acid phosphatase. In uncropped soil, copper was the least effective in changing the activity of arylsulfatase and acid phosphatase. All of the tested enzymes were less resistant to copper contamination in cropped than in uncropped soil. In soil planted with oat, β-glucosidase was the most resistant and arylsulfatase was the least resistant enzyme to copper contamination. In samples sown with spring rape, the analogous enzymes were arylsulfatase and alkaline phosphatase. In yellow lupine treatments, alkaline phosphatase was the most and β-glucosidase was the least resistant enzyme.

PL

W doświadczeniu wazonowym badano wpływ zanieczyszczenia gleby (piasku gliniastego oraz gliny piaszczystej) miedzią w dawkach: 0, 150, 450 mg Cu·kg-1 s.m. gleby na aktywność: β-glukozydazy (EC 3.2.1.21), fosfatazy kwaśnej (EC 3.1.3.2), fosfatazy alkalicznej (EC 3.1.3.1) i arylosulfatazy (EC 3.1.6.1) w glebie. Określono także odporność tych enzymów na zanieczyszczenie miedzią. Glebę zanieczyszczano chlorkiem miedzi. Badania prowadzono w 5 powtórzeniach, w dwóch seriach. W pierwszej serii doświadczenia gleba była nieobsiana roślinami, w drugiej — obsiana. Roślinami doświadczalnymi były: owies, rzepak jary i łubin żółty. W 25. i 50. dniu trwania eksperymentu oznaczono w próbkach glebowych aktywność enzymów glebowych. Stwierdzono, że zanieczyszczenie gleby miedzią w zakresie od 150 mg do 450 mg·kg-1 gleby istotnie hamuje jej aktywność biochemiczną. Testowane enzymy, pod względem wrażliwości na miedź, można uszeregować następująco: fosfataza alkaliczna > arylosulfataza > fosfataza kwaśna > β-glukozydaza. Odporność enzymów na działanie miedzi zależała od gatunku uprawianej rośliny, rodzaju gleby i sposobu jej użytkowania. W glinie piaszczystej miedź wywołała najmniejsze zmiany w aktywności fosfatazy kwaśnej i fosfatazy alkalicznej, natomiast w piasku gliniastym — β-glukozydazy oraz arylosulfatazy. W glebie nieobsianej roślinami miedź wywoływała najmniejsze zakłócenia w aktywności arylosulfatazy i fosfatazy kwaśnej. W glebie obsianej roślinami wszystkie testowane enzymy były mniej odporne na zanieczyszczenie miedzią niż w glebie obsianej. Najbardziej odpornym enzymem na działanie miedzi pod uprawą owsa była β-glukozydaza, a najmniej — arylosulfataza, pod uprawą rzepaku jarego, odpowiednio — arylosulfataza i fosfataza alkaliczna, natomiast pod uprawą łubinu żółtego najbardziej odporna była fosfataza alkaliczna, a najmniej β-glukozydaza.

Słowa kluczowe

EN

beta-glucosidase activity; arylsulphatase; phosphatase; soil contamination; copper; resistance

• Wydawca: -
• Czasopismo: Journal of Elementology
• Rocznik: 2010
• Tom: 15
• Numer: 1
• Opis fizyczny: p.213-226,ref.

Twórcy

autor

Wyszkowska J

• University of Warmia and Mazury in Olsztyn, pl.Lodzki 3, 10-727 Olsztyn, Poland

autor

Kucharski M.

autor

Kucharski J.

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