Preliminary study of the RBP4, EGF and PTGS2 genes polymorphism in pigs and its association with reproduction traits of sows

• Autorzy: Korwin-Kossakowska A , Kapelanski W. , Bocian M. , Kamyczek M. , Sender G.
• Języki publikacji: EN

Abstrakty

EN

Genes of retinol-binding protein 4 (RBP4), epidermal growth factor (EGF) and prostaglandin-endoperoxide synthase 2 (PTGS2) were examined as candidate genes for reproductive traits in sows. The material included a total of 300 sows of the Polish Landrace (n=86), Polish Large White (n=64)and commercial Line 990 (n=150). Included in the study was the total number of piglets born (TNB),number of piglets born alive (NBA), number of piglets on day 21 of age (NP21), number of piglets weaned (NPW), litter weight on day 21 of age (LW21), litter weight at weaning. (LWW) and farrowing interval (FI). Calculations were based on records available for two farrowings (1st and 2nd) from each sow.The RBP4 genotype was found to have a significant effect on LW21 and LWW (P≤0.05 and P≤0.01,respectively). No significant effect of this genotype was identified for litter size traits (TNB, NBA).The effect of EGF genotype was found significant on TNB (P≤0.01) and NBA (P≤0.05). The A/A animals showed the highest reproductive efficiency compared to those of B/B and A/B genotypes.No significant effects of the PTGS2 genotype were found due to a total absence of one of the homozygous genotypes at this locus.

PL

Geny RBP4 (retinol-binding protein 4), EGF (epidermal growth factor) i PTGS2 (prostaglandin-endoperoxide synthase 2) badano jako geny kandydujące cech reprodukcyjnych macior. Materiał stanowiło 300 macior ras pbz , wbp i linii syntetycznej 990 (odpowiednio n = 86, 64 i 150) Na podstawie danych pochodzących z dwóch pierwszych miotów, analizowano zależność między genotypami badanych białek a poziomem wybranych cech związanych z liczebnością i masą miotu, a także okresem międzymiotu. Istotne zależności stwierdzono między genotypami RBP4 a masą miotu i między genotypami EGF a liczebnością miotu.W obrębie genu PTGS2 nie zaobserwowano żadnych istotnych zależności. Jednak w przypadku PTGS2, w testowanym materiale stwierdzono występowanie homozygot tylko jednego typu.

Słowa kluczowe

EN

pig; gene polymorphism; RBP4 gene; animal breeding; EGF gene; epidermal growth factor gene; PTGS2 gene; reproduction trait; sow

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2005
• Tom: 23
• Numer: 2
• Opis fizyczny: p.95-105,fig.,ref.

Twórcy

autor

Korwin-Kossakowska A

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Kapelanski W.

autor

Bocian M.

autor

Kamyczek M.

autor

Sender G.

Bibliografia

• 1. BRIEF S., CHEW B.P., 1985 – Effects of vitamin A and B-carotene on reproductive performance In gilts. Journal of Animal Science 60, 998-1004.
• 2. CASSADY J.P., 1999 – Identification of quantitative trait loci affecting reproduction in pigs. PhD dissertation. University of Nebraska, Lincoln.
• 3. DEKKERS J.C.M., 2004 – Commercial application of marker-and gene-association in livestock: Strategies and lessons. Journal of Animal Science 82, 313-328.
• 4. DROGEMüLLER C., HAMANN H., DISTL O., 2001 – Candidate gene markers for litter size In different German pig lines. Journal of Animal Science 79, 2565-2570.
• 5. GLADNEY C.D., MARTINEZ V.G., BRUMBAUGH K.A., DEGROOT B.J., LINVILLE R.C.,OOMMEN A. M., HUEBINGER R.M., MESSER L.A., ALLAN M. F., POMP D.,1999 – Mapping of the Prostaglandin-Endoperoxide Synthase 2 (PTGS2) gene to porcine chromosome 9 and bosine chromosome 16 by linkage analysis using novel PCR-RFLP. Journal of Animal Science 77(3), 787-8.
• 6. HADLEY M.E., 1996 – Endocrinology. 4th ed. Prentice Hall, Upper Saddle River, NJ.
• 7. HARNEY J.P., OTT T.L., GEISERT R.D., BAZER F.W., 1993 – Retinol binding protein gene expression in cyclic and pregnant endometrium of pigs, sheep and cattle. Biology of Reproduction 49, 1066-1073.
• 8. HARNEY J.P., MIRANDO M.A., SMITH L.C., BAZER F.W., 1990 – Retinol-binding protein: A major secretory product of the pig conceptus. Biology of Reproduction 42, 523-532.
• 9. ISLER B.J., IRVIN K.M., NEAL S.M., MOELLER S.J., 2002 – Examination of the relationship between the paternally expressed gene 3 and reproductive tract components in swine. Proceedings of the 7th Word Congress on Genetics Applied to Livestock Production, August 19-23, Montpellier,France. Paper 08-28.
• 10. JIANG Z., ROBINSON J.A.B., VERRINDER GIBBINS A.M., GIBSON J.P., ARCHIBALD A.L.,HALEY C.S., 2002 – Mapping of QTLs for prolificacy traits on SSC 8 using a candidate gene approach. Proceedings of the 7th Word Congress on Genetics Applied to Livestock Poduction, August 19-23, Montpellier, France. Paper 08-02.
• 11. KAWASAKI E. S., 1990 – Sample preparation from blood, cells and other fluids. In: PCR Protocols; A guide to methods and applications. (M.A. Innis, D.H.Gelfand, J.J. Sninsky, T.J. White, Eds.) Academy Press, New York, 146-152.
• 12. KING A.H., JIANG Z., GIBSON J.P., HALEY CH.S., ARCHIBALD A.L., 2003 – Mapping Quantitative Trait Loci affecting female reproductive traits on porcine chromosome 8. Biology of Reproduction 68, 2172-2179.
• 13. KIRKPATRICK B.W., 2002 – QTL and candidate gene effects on reproduction in livestock: Progress and Prospects. Proceedings of the 7th Word Congress on Genetics Applied to Livestock Production,August 19-23, Montpellier, France. Paper 08-01.
• 14. LIM H., PARIA B.C., DAS S.K., DINCHUK J.E., LANGENBACH R, TRZASKOS J.M., DEY S.K., 1997 – Multiple female reproductive failures in cyclooxygenase 2-deficient mice. Cell 91(2),197-208.
• 15. LINVILLE R.C., POMP D., JOHNSON R.K., ROTHSCHILD M.F., 2001 – Candidate gene analysis for loci affecting litter size and ovulation rate in swine. Journal of Animal Science, 79, 60-67.
• 16. MENDEZ E.A., MESSER L.A., LARSEN N.J., ROBIC A., ROTHSCHILD M.F., 1999 – Epiderma Growth Factor Map to pig chromosome 8. Journal of Animal Science 77, 494-495.
• 17. MESSER L.A., WANG L., LEGUALT C., ROTHSCHILD M.F., 1996 – Mapping and investigation of candidate genes for litter size in French Large white pigs. Animal Genetics 27 (Suplement 2), 101-119.
• 18. OLLIVIER L., MESSER L.A., ROTHSCHILD M.F., LEGAULT C., 1997 – The use of selection experiments for detecting quantitative trait loci. Genetic Resaurces 69, 227-232.
• 19. RATHJE T.A., ROHRER G.A., JOHNSON R.K., 1997 – Evidence for quantitative trait loci affecting ovulation rate in pigs. Journal of Animal Science 75, 1486-1494.
• 20. ROBERTS R.M., XIE S., TROUT W.E., 1993 – Embryo-uterine interaction in pigs during week 2 of pregnancy. Journal of Reproduction and Fertility, Supplement 48, 171-186.
• 21. ROHRER G.A., FORD J.J., WISE T.H., VALLET J.L., CHRISTENSON R.K., 1999 – Identification of quantitative trait loci affecting female reproductive traits in a multigeneration Meishan-white composite swine population. Journal of Animal Science 77, 1385-1391.
• 22. ROTHSCHILD M.F., MESSER L., DAY A., WALES R., SHORT T., SOUTHWOOD O., PLASTOW G., 2000 – Investigation of the retinol-binding protein 4 (RBP4) gene as a candidate gene for increased litter size in pigs. Mammalian Genome 11,75-77.
• 23. ROTHSCHILD M.F., 1998 – Identification of quantitative trait loci and interesting candidate genes In the pig: Progress and prospects. Proceedings of 6th Word Congress on Genetics Applied to Livestock Production , Armidale, NSW, Australia. 26, 403-409.
• 24. TROUT W.E., MCDONNELL J.J., KRAMER K.K., BAUMBACH G.A., ROBERT R.M. 1991 – The retinol-binding protein of the expanding pig blastocyst: molecular cloning and expression In trophectoderm and embryonic disc. Molecular Endocrinology 5, 1533-1540.
• 25. YELICH J.V., POMP D., GEISERT R.D., 1997 – Detection of transcripts for retinoic acid receptors, retinol-binding protein and transforming growth factors during rapid trophoblastic elongation in the porcine conceptus. Biology of Reproduction, 57(2), 286-294.
• 26. WILKIE P.J., PASZEK A.A., BEATTIE C.W., ALEXANDER L.J., WHEELER M.B., SCHOOK L.B., 1999 – A genomic scan of porcine reproductive traits reveals possible quantitative trait loci (QTLs) for number of corpora lutea. Mammalian Genome 10, 573-578.
• 27. WOLLENHAUPT K., TOMEK W., BRUSSOW K.P., TIEMANN U., VIERGUTZ T., SCHNEIDER F., NURNBERG G., 2002 – Effects of ovarian steroids and epidermal growth factor (EGF) on expression and bioactivation of specific regulators of transcription and translation in oviductal tissue in pigs. Reproduction 123 (1), 87-96.
• 28. WILSON M.E., FAHRENKRUG S.C., SMITH T.P., ROHRER G.A., FORD S.P., 2002 – Differentia expression of cyclooxygenase-2 around the time of elongation in the pig conceptus. Animal Reproduction Science 71 (3-4), 229-237.