Zmiany wrazliwosci Staphylococcus aureus na bakteriobojcze dzialanie teikoplaniny zwiazane z obecnoscia roznych profagow w genomie bakteryjnym

• Autorzy: Mlynarczyk G. , Mlynarczyk A. , Zabicka D. , Luczak M.
• Języki publikacji: PL

Abstrakty

PL

Oceniano wpływ obecności profagów wbudowanych w genom szczepu NCTC 8325-4 na wartości MIC i MBC teikoplaniny. Bakteriofagi otrzymane były z wielolekoopornych szczepów Staphylococcus aureus.

EN

The influence exerting by lysogeny state on the susceptibility of Staphylococcus aureus to bactericidal action of teicoplanin was studied. In this aim the standard, non-lysogenic, bacteriophage-free S.aureus NCTC 8325-4 strain was lysogenized with 10 different, bacteriophages obtained in our laboratory. All bacteriophages were derived from multiresistant S. aureus strains and all were able to convert staphylokinase. For all derivatives MBCs and MICs of teicoplanin were determined. In the case of four strains the ratio MBC/MIC showed the presence of tolerance to teicoplanin (MBC/MIC ≥32) and was significantly higher than in the case of the parent strain NCTC8325-4. In the case of two strains this ratio was smaller than for parent strain. Only small correlation with our previous results obtained for vancomycin was observed.

Słowa kluczowe

PL

bakteriofagi; dzialanie bakteriobojcze; szczepy bakteryjne; antybiotyki; tolerancja na penicyline; genomy; teikoplanina; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 3
• Opis fizyczny: s.203-207,tab.,bibliogr.

Twórcy

autor

Mlynarczyk G.

• Akademia Medyczna, Warszawa

autor

Mlynarczyk A.

autor

Zabicka D.

autor

Luczak M.

Bibliografia

• 1 .Dela HigueraA, Castillo A, Gutierrez J i inni. In-vitro susceptibility, tolerance and glycocalyx production in Streptococcus mutans. J Antimicrob Chemother 1997; 40: 359-63.
• 2. Filipe SR, Severina E, Tomasz A. The murMN operon: a functional link between antibiotic resistance and antibiotic tolerance in Streptococcus pneumoniae. Proc Natl Acad Sci USA. 2002; 99: 1550-55.
• 3. Gutmann L, Al-Obeid S, Billot-Klein D i inni. Penicillin tolerance and modification of lipoteichoic acid associated with expression of vancomycin resistance in VanB-type Enterococcus faecium D366. Antimicrob Agents Chemother 1996; 40: 257-9.
• 4. Koch AL. Autolysis control hypotheses for tolerance to wall antibiotics. Antimicrob Agents Chemother 2001; 45: 2671-5.
• 5. May J, Shannon K, King A, French G. Glycopeptide tolerance in Staphylococcus aureus. J Antimicrob Chemother 1998; 42(2): 189-97
• 6. Młynarczyk A, Młynarczyk G, Żabicka D i inni. Bacteriophages of the serologic group A converting synthesis of staphylokinase and beta toxin in S.aureus. Med Dośw Mikrobiol 1993; 45: 158.
• 7. Młynarczyk G, Młynarczyk A, Jeljaszewicz J. Bacteriophages of the serologic group F converting synthesis of fibrinolisine and beta toxin in S. aureus. Med Dośw Mikrobiol 1995; 47: 149-54.
• 8. Młynarczyk G, Młynarczyk A, Jeljaszewicz J. Bacteriophages of the serologic group В converting synthesis of fibrinolisine in S.aureus. Med Dośw Mikrobiol 1995; 47:141-7.
• 9.Młynarczyk G, Młynarczyk A, Żabicka D, Jeljaszewicz J. Lysogenic conversion as a factor influencing the vancomycin tolerance phenomenon in Staphylococcus aureus. J Antimicrob Chemother 1997; 40: 136-7.
• 10.National Committee for Clinical Laboratory Standarts. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 4th ed. Approved standart M7-A4. National Committee for Clinical Laboratory Standards, Wayne, Pa 1997.
• 11.Navarre WW, Ton-That H, Faull KF, Schneewind O. Multiple enzymatic activities of the murein hydrolase from staphylococcal phage phill. Identification of a D-alanyl-glycine endopeptidase activity. J Biol Chem. 1999; 274:15847-56.
• 12. Normark HB, Normark S. Antibiotic tolerance in pneumococci. Clin Microbiol Infect 2002; 8: 613-22.
• 13. Ramanculov E, Young R. An ancient player unmasked: T4 rl encoded at-specific antiholin. Mol Microbiol 2001; 41:575-83.
• 14. Robertson GT, Zhao J, Desai BV i inni. Vancomycin tolerance induced by erythromycin but not by loss of vncRS, vex3, or pep27 function in Streptococcus pneumoniae. J Bacterid 2002; 184: 6987-7000.
• 15. Sakoulas G, Eliopoulos GM, Moellering RC Jr i inni. Accessory gene regulator (agr) locus in geographically diverse Staphylococcus aureus isolates with reduced susceptibility to vancomycin. Antimicrob Agents Chemother 2002 46: 1492-502.
• 16.Wang IT, Smith DL, Young R. Holins the protein clodks of bacteriophage infections. Annu Rev Microbiol 2000; 54: 799-825.
• 17. Young R, Blasi U. Holins: form and function in bacteriophage lysis. FEMS Microbiol Rev 1995; 17: 191-205.
• 18. Von Hunolstein C, Scopetti F, Efstratiou A, Engler K. Penicillin tolerance amongst non-toxigenic Corynebacterium diphtheriae isolated from cases of pharyngitis. J Antimicrob Chemother. 2002; 50: 125-8.
• 19. Walters MC 3rd, Roe F, Bugnicourt A i inni Contributions of antibiotic penetration, oxygen limitation, and low metabolic activity to tolerance of Pseudomonas aeruginosa biofilms to ciprofloxacin and tobramycin. Antimicrob Agents Chemother 2003; 47: 317-23.
• 20.Zelenitsky S, Franczuk C, Fine A i inni Antibiotic tolerance of peritoneal bacterial isolates in dialysis fluids. J Antimicrob Chemother 2002; 49:863-66.