TEM investigation of tannins and chloroplast structure in needles of damaged silver fir trees [Abies alba Mill.]

• Autorzy: Bacic T. , Ljubesic N. , Uzarevic Z. , Grgic L. , Rosa J.
• Języki publikacji: EN

Abstrakty

EN

As part of investigations on the effect of air pollution on plant anatomy, we examined the condition of tannins and chloroplast structure in damaged fir trees (Abies alba Mill.) at Risnjak National Park. Two populations were chosen for needles sampling: one at the Risnjak site with trees having 20%, 45% and 85% damage, and the other at the Donja Dobra site (control locality) with relatively healthy trees having 5% to 10% damage. Current and previous-year needles were fixed with glutaraldehyde and osmium tetroxide, and then the condition or ultrastructure of tannins and chloroplasts were examined by transmission electron microscopy (TEM). The tannins were shown to be granular and arranged in thin or thick ribbons. The chloroplasts were first somewhat rounded and then round or irregular in shape, with reduced and swollen thylakoids, especially those of the grana, increased numbers of plastoglobuli, large lipid droplets/accumulations, and vesiculation of the cytoplasm. These symptoms were more frequent in previous-year than in current-year needles. All these alterations can be attributed to air pollution.

Słowa kluczowe

EN

transmission electron microscopy; investigation; tannin; chloroplast structure; needle; silver fir tree; Abies alba; chloroplast; air pollution

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2004
• Tom: 46
• Opis fizyczny: p.145-149,fig.,ref.

Twórcy

autor

Bacic T.

• J.J.Strossmayer University, L.Jagera 9, 31000 Osijek, Croatia

autor

Ljubesic N.

autor

Uzarevic Z.

autor

Grgic L.

autor

Rosa J.

Bibliografia

• Amantaurić A. 1981. Metoda za odreevanje sumpor-dioksida i dima u atmosferi. Zakoni i propisi. Zaštita atmosfere 9: 117-120.
• Bačić T, and Popović Ž. 1998. Preliminary report on epicuticular wax surface condition on stomata of Abies alba Mill, needles from Risnjak National Park in Croatia. Acfa Biologica Cracoviensia Series Botanica 40: 25-31.
• Bačić T, Užarević Z, Roša J, Grgić LJ, and Popović Ž. 2003. Chlorophylls and carotenoids in the needles of damaged fir (Abies alba Mill.) trees from Risnjak National Park in Croatia. Acta Biologica Cracoviensia Series Botanica: 45 /2: 87-92.
• Behrens A, Nagamitsu M, Knicker H, and Kögel-Knabner I. 2003. MALDI-TOF mass spectrometry and PSD fragmentation as means for the analysis of condensed tannins in plant leaves and needles. Phytochemistry 62: 1159-1170.
• Beckman CH, Muller WC, and McHardy WE. 1972. The localization of stored phenols in plant hairs. Physiology Plant Pathology 2: 69-74.
• Davies ME. 1972. Polyphenol synthesis in cell suspension cultures of Paul’s Scarlet rose. Planta 104: 50-65.
• Durbešić P, and Kerovec I. 1990. Poziv za opstanak šuma. Ekološki glasnik 3-4: 31-32.
• Engelbrecht HP, and Louw W. 1973. Hydrogen fluoride injury in sugar-cane: Some ultrastructural changes. In: Proceedings of the Third International Clean Air Congress. A 157- 159. VDI- Verlag GmbH, Düsseldorf.
• Feeny P. 1970. Seasonal changes in oak leaf tannins and nutrients as a cause of spring feeding by winter moth caterpillars. Ecology 51: 565-581.
• Fischer F, Kramer D, and Ziegler H. 1973. Elektronenmikroskopische Untersuchungen SO2 - begaster Blätter von Vicia faba L. Beobachtungen am Chloroplasten mit akuter Schädigung. Protoplasma 76: 83-96.
• Glavaš M. 1987. Nadelkrankheiten der Veistane in Kroatien. 5th IUFRO - Tannensymposium: 395-400.
• Glavaš M. 1992a. Diseases of the fir on the Velebit. 8th IUFRO -Tannensymposium: 213-218.
• Glavaš M. 1992b. Cytospora pinastri Fries. uzročnik osipanja iglica jele (Abies alba Mill.). Šumarski list 66: 79-86.
• Godzik S, and Knabe W. 1973. Vergleichende elektronmikroskopische Untersunchungen der Feinstruktur von Chloroplasten einiger Pinus Arten aus den Industriegebieten en der Ruhr und Oberschlesien. In: Preceedings of the Third International Clean Air Congress. A 161-170. VDI - Verlag GmbH, Düsseldorf.
• Godzik S, and Sassen MA. 1974. Einwirkung von SO2 auf die Feinstruktur der Chloroplasten von Phaseolus vulgaris. Phytopatologia 79: 155-159.
• Komlenović N, and Rastovski P. 1992. Research of nutritional status of silver fir (Abies alba Mill.) of different damage degree. 6th IUFRO - Tannensymposium: 183-190.
• Kouki M, and Manestas Y. 2002. Resource availability affects differentially of gallotannins and condensed tannins in Ceratonia siliqua. Biochemical Systematics and Ecology 30: 631-639.
• KukkolaE, Huttunen S, Bäck J, and Rautio P. 1997. Scots pine needle injuries at subarctic industrial sites. Trees - structure and function 11: 378-387.
• Kušan V, Kalafadžić Z, Pernar R, and Horvatić Z. 1993. Procjena oštećenosti šuma u nacionalnom parku "Risnjak" fotointerpretacijom infracrvenih kolornih aerosnimaka. Zbornik radova: 40 godina nacionalnog parka "Risnjak" (1953-1993). "Stuba", Zagreb.
• Maie N, Behrens A, Knicker H, and Kögel-Knabner I. 2003. Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves. Soil Biology and Biochemistry 35: 577-589.
• Masuch C, Weinert H, and Guderian R. 1973. Wirkungen von Chlorowasserstoff und Schwefeldioxid auf die II der Ultrastruktur Chloroplasten von Spinacia oleracea L. In: Proceedings of the Third International Clean Air Congress. A 160-163. VDI-Verlag GmbH, Düsseldorf.
• Miko S, Peh Z, Bukovec D, Prohić E, and Kastmüller Z. 2000. Geochemical baseline mapping and lead pollution assessment of soils on the karst in western Croatia. Natura Croatica 9: 41-59.
• Mlodzinowski F, and Bialobok S. 1977. The effect of sulphur dioxide on ultrastructural organization of Larch needles. Acta Societatis Botanicorum Poloniae 46: 629-634.
• Opalički K. 1972. Spektar nekih štetnika jele u SR Hrvatskoj. Šumarski list 3-4: 131-145.
• Prpić B. 1990. Kemijska klima u odnosu na propadanje šuma u Hrvatskoj. Ekološki glasnik 3-4: 26-30.
• Roša J. 2001. Praćenje šumskih ekosustava. Hrvatske šume, Zagreb.
• Soikkeli S. 1978. Seasonal changes in mesophyll ultrastructure of needles of Norway spruce (Picea abies). Canadian Journal of Botany 56: 1932-1940.
• Soikkeli S. 1980. Ultrastructure of the mesophyll in Scots Pine and Norway Spruce: Seasonal variation and molarity of the fixative buffer. Protoplasma 103: 241-252.
• Soikkeli S. 1981a. Comparison of cytological injuries in conifer needles from several polluted industrial environments in Finland. Annales Botanici Fennici 18: 47-61.
• Soikkeli S. 1981b. A review of the structural effects of the air pollution on mesophyll tissue on the plants at the light and transmission electron microscope level. Savonia 4: 11-54.
• Soikkeli S, and Tuovinen T. 1979. Damage in mesophyll ultrastructure of needles of Norway spruce in the industrial environments in central Finland. Annales Botanici Fennici 16: 50-64.
• Spaić J. 1972. Prilog poznavanje parazita jelinog igličara Argyresthia fundella T. R. Acta Entomologica Yugoslavica 8: 1-2.
• Tuovinen T. 1979. Damage in mesophyll ultrastructure of needles of Norway spruce in two industrial environments in central Finland. Annales Botanici Fennici 16: 50-64.
• Vrbek B, and Gašparac M. 1992. Novi nalazi imisije teških metala u Nacionalnom Parku "Risnjak". Radovi 27: 65-75.
• Wellburn AR, Majernik O, and Wellburn FAM. 1972. Effects of SO2 and NO2 polluted air upon ultrastructure of chloroplasts. Environmental Pollution 3: 37-49.