Zastosowanie cytometrii przeplywowej w badaniach nad patogeneza pomoru swin wywolanego przez szczepy o roznej zjadliwosci

• Autorzy: Markowska-Daniel I , Winnicka A. , Klucinski W. , Pejsak Z.
• Języki publikacji: PL

Abstrakty

EN

The aim of the experiment was a FACS analysis of the immune response of porcine leukocyte populations involved in the infection of piglets caused by virulent, low virulent and vaccine strains of classical swine fever virus (CSFV). Twenty piglets were infected with Washington (W), Alfort (A) and Cellpest (C) strains of CSFV, respectively. The peripheral blood populations of leukocytes were analysed by the following set of specific monoclonal antibodies; anti-CD45, CD14, CD2, CD4, CD8, CD4-CD8-TcRγδ+, and anti CD19. Typical clinical and anatomopathological symptoms of CSFV infections were observed in piglets infected with W and A strains, the atenuated vaccine strain did not cause viraemia. In groups infected with W and A strains 40% and 20% of the piglets died, respectively. This was accompanied by a significant change of the leukocyte population. The percentage of lymphocytes decreased significantly 72 hrs after infection. Moreover, lymphopenia, the decreased number of В and T lymphocytes, as well as monocyte and trombocyte populations were observed. Contrary to those, the percent of granulocytes gradually increased. The number of the erytrocytes and the measured parameters of the red blood cells were not significantly different in comparison with the control piglets. At the beginning of the infection the percentage of Th and observed TcRγδ+ lymphocytes increased, then 96 hrs later gradually decreased, while Ts population increased till day 11 of the experiment. Piglets infected with atenuated strain reacted differently, compared with groups infected with W and A strains: an increased number of leucocytes, lymphocytes, granulocytes, trombocytes, as well as В lymphocytes were observed. The influence of the experimental infection on cell immunity were demonstrated by the increased number of T lymphocytes, especially Th population and decreased number of Ts population. The results observed during the experimental infections pf piglets indicate that the immunological response varied greatly depending on the virulency of the strain used for inoculating the swine.

Słowa kluczowe

PL

odpowiedz immunologiczna; czynniki chorobotworcze; trzoda chlewna; infekcja wirusowa; szczepy wirusowe; uklad bialokrwinkowy; patogeneza; zjadliwosc; wirus pomoru klasycznego swin; pomor swin; choroby zwierzat; cytometria przeplywowa

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 1998
• Tom: 54
• Numer: 04
• Opis fizyczny: s.258-264,rys.,bibliogr.

Twórcy

autor

Markowska-Daniel I

• Panstwowy Instytut Weterynaryjny, Al.Partyzantow 57, 24-100 Pulawy

autor

Winnicka A.

autor

Klucinski W.

autor

Pejsak Z.

Bibliografia

• 1. Bauman J. G., Bentvelzen P.: Cytometry 9, 517, 1988.
• 2. Bianchi A. T., Zwart R. J., Jeurissen S. H., Moonen-Leusen H. W.: Vet. Immun. Immunopathol. 33, 201, 1992.
• 3. Bolin S. R., McClurkin A. W., Coria M. F.: Am. J. Vet. Res. 46, 884, 1985.
• 4. Cheville N. F., Mengeling W. L.: Lab. Invest. 20, 261, 1969.
• 5. Corthier G.: Am. J. Vet. Res. 39, 1841, 1978.
• 6. Depner K. R., Greiser-Wilke I., Liess B., Moennig V.: Proc. Int. Symp. Diagnostic procedures and measures to control classical swine fever in domestic pigs and the European wild boar. Puławy, 1996, s. 43.
• 7. Dahle J., Liess B., Frey H. R.: J. Vet. Med. В 34, 729, 1987.
• 8. Edwards S.: Proc. Int. Symp. Diagnostic procedures and measures to control classical swine fever in domestic pigs and the European wild boar. Puławy, 1996, s. 25.
• 9. Ehrensperger F.: Immunological aspects of the infection, w: Classical swine fever and related infections, red. B. Liess. Martinus Nijhoff Publ., Boston Dordrecht Lancaster, 1988.
• 10. Ellis J. A., Davis W. C., Belden E. L., Pratt D. L., Vet. Pathol. 25, 23, 1988.
• 11. Hermanns W., Trautwein G., Meyer H., Liess B.: Zbl. Med. Vet. В 28, 669, 1981.
• 12. Jakóbisiak M.: Immunologia, Wyd. PWN Warszawa, 1996, s. 167.
• 13. Kamolsiriprichaiporn S., Hooper P. T., Morrissy C. J., Westbury H. A.: Austr. Vet. J. 69, 10, 1992.
• 14. Kawiak J., Skierski J.: Post. Biol. Kom. 19, 239, 1992.
• 15. Kawiak J., Hoser G.: Post. Biol. Kom. 21, 39, 1994.
• 16. Kraj P.: Immunologia Polska, 16, 91, 1991.
• 17. Liess B.: Proc. Regional Biotechnology Workshop on diagnosis of classical swine fever, Puławy, 1994, s. 1-6.
• 18. Loan R. W., Rodabaugh D. E.: Am. J. Vet. Res. 27, 1333, 1966.
• 19. Lowings P., Ibata G., Needham J., Paton D.: J. Gen. Virol. 77, 1311, 1996.
• 20. Narita M., Kawashima K., Shimizu M.: J. Comp. Pathol. 114, 257, 1996.
• 21. Niezabitowski A., Lackowska B.: Diagn. Lab. 27, 1, 1991.
• 22. Pajdak W.: Immunologia Polska 21, 269, 1996.
• 23. Pejsak Z., Truszczyński M.: Rozpoznawanie i zwalczanie chorób świń. Wyd. IWet., Puławy, 1995, s. 27-33.
• 24. Pejsak Z.: Medycyna Wet. 51, 191, 1995.
• 25. Reddehase M. J., Saalmuller A., Hirt W.: Curr. Top. Microbiol. Immunol. 173, 113, 1991.
• 26. Remond M., Plateau E., Cruciere C.: Zbl. Vet. Med. В 28, 743, 1981.
• 27. Ressang A.: Zbl. Vet. Med. В 20, 272, 1973.
• 28. Saalmuller A., Reddehase M. J.: Res. Vet. Sci. 45, 311, 1988.
• 29. Saalmuller A., Hirt W., Reddehase M. J.: Eur. J. Immunol. 19, 201, 1989.
• 30. Shimizu M., Yamada S., Kawashima K., Ohashi S., Shimizu S., Ogawa T.: Vet. Immun. Immunopathol. 50, 19, 1996.
• 31. Susa M., Konig M., Saalmuller A., Reddehase M. J., Thiel H.: J. Virol. 66, 1171, 1992.
• 32. Terpstra C.: Epizootoplogy of hog cholera, w Classical swine fever and related infections, red. B. Liess, Martinus Nijhoff Publ., Boston Dordrecht Lancaster, 1988, s. 201-216.
• 33. Trautwein G.: Pathology and pathogenesis of the disease, w: Classical swine fever and related infections, red. B. Liess. Martinus Nijhoff Publ., Boston Dordrecht Lancaster, 1988, s. 27-53.
• 34. Van der Molen E. J., Van Oirschot J. T.: Zbl. Med. Vet. В 28, 89, 1981.
• 35. Van OirschotJ. T.: Vet. Microbiol. 4, 321, 1983.
• 36. Van Oirschot J. T., De Jong D., Huffels N.: Vet. Micriobiol. 8, 81, 1983.
• 37. Westergaard J. M.: Proc. Int. Symp. Diagnostic procedures and measures to control classical swine fever in domestic pigs and the European wild boar. Puławy, 1996, s. 119.
• 38. Winnicka A.: Wartości referencyjne podstawowych badań laboratoryjnych w weterynarii. Wyd. SGGW, Warszawa, 1997.