Zmiany aktywnosci aprotyniny w chlodzonych i mrozonych plucach bydlecych

• Autorzy: Hajduk E
• Języki publikacji: PL

Abstrakty

EN

Aprotinin - a proteinase inhibitor, is a polipeptyde found in bovine organs. For commercial purposes it is isolated only from bovine lungs, pancreas or parotid glands. Aprotinin is widely used as a valuable tool for studying protein/protein interactions and protein conformation at the molecular level. It has also become a valuable drug for the treatment of various diseases, e.g. hyperfibrinolytic hemorrhage, traumatic-hemorrhagic shock and acute pancreatitis. The purpose of these studies was to determine the changes of aprotinin activity in bovine lungs during frozen (-12 and -28°C for 14 months) and chilling (4°C for 17 days) storage. In the latter, half of the raw material was pre-stored in -28°C for 1 month. The photometric assay was made to determine aprotinin activity which was then measured using kallikrein inactivator units (KIU). It has been found that neither frozen nor chilling storage reduce the inhibitor activity. The growth of tissue extractibility was also observed as a result of the freezing process, causing an increase of aprotinin activity in analysed samples.

Słowa kluczowe

PL

aprotynina; przechowywanie; aktywnosc; surowce farmaceutyczne; pluca bydlece; mrozenie; chlodzenie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2000
• Tom: 56
• Numer: 05
• Opis fizyczny: s.320-322,tab.,bibliogr.

Twórcy

autor

Hajduk E

• Akademia Rolnicza, ul. Podluzna 3, 30-239 Krakow

Bibliografia

• 1. Anderer F A., Hornie SStrukturuntersuchungen am Kallikrein - Inaktiva- tor aus Rinderlunge. I. Molekulargewicht, Endgruppenanalyse und Ami- nosáure Zusammensetzung, Z. Naturforsch. 1965, 20b, 457-460.
• 2. Frey E. K„ Kraut H., Werle E.: Das Kallikrein - Kinin System und seine Inhibitoren. F. Enke - Verlag, Stuttgart 1968, s. 114.
• 3. Fritz H., Trautschold/., Werle E.: w: Methoden der Enzymatischen Analyse. Bergmeyer H. U., Verlag Chemie, Weinheim/Bergstr. 1974, s. 1105.
• 4. Fritz H., Wunderer G.: Biochemistry and applications of aprotinin, the kallikrein inhibitor from bovine organs. Drug Research/Arzneim.-Forsch. 1983, 33, 479-494.
• 5. Hajduk E.: Wpływ czynników biologicznych na jakość płuc bydlęcych jako surowca dla przemysłu farmaceutycznego. Medycyna Wet. 1998,54,702-705.
• 6. Kassel B.: Bovine trypsin - kallikrein inhibitor (Kunitz inhibitor, basic pancreatic trypsin inhibitor, polyvalent inhibitor from bovine organs). Meth. Enzym. 1970, 19, 844-852.
• 7. Vogel R.: w: Bradykinin, Kallidin and Kallikrein - Handb. Exp. Pharm., T. 25 Suppl., Erdos E. G., Springer - Verlag, Berlin 1979, s. 163.
• 8. Vogel R., Trautschold I., Werle E.: Natural Proteinase Inhibitors. Academic Press, New York 1968, s. 76.
• 9. Wiithrich K, Wagner G.: Nuclear magnetic resonance of labile protons in the basic pancreatic trypsin inhibitor. J. Mol. Biol. 1979, 130, 1-18.