Wykorzystanie elektroforezy kapilarnej w analizie zywnosci

• Autorzy: Cieslik E , Niedospial A , Mickowska B

Warianty tytułu

EN

Application of capillary electrophoresis in the analysis of food products

• Języki publikacji: PL

Abstrakty

PL

Wysokosprawna elektroforeza kapilarna (CE) jest metodą stosowaną do rozdziału, identyfikacji i ilościowego oznaczania wielu związków w produktach spożywczych. Dzięki swej różnorodności techniki elektroforezy kapilarnej mogą być wykorzystywane do oznaczania zawartości zarówno związków wielkocząsteczkowych, np. białek czy fragmentów DNA, jak i drobnocząsteczkowych: aminokwasów, węglowodanów, witamin, flawonoidów, jonów nieorganicznych oraz kwasów organicznych. Zastosowanie tej metody w laboratoriach kontroli żywności może znacznie ułatwić weryfikowanie deklarowanego i rzeczywistego składu produktów żywnościowych.

EN

The highly efficient Capillary Electrophoresis (CE) is a method used for the separation, identification, and quantitative analysis of many compounds contained in food products. Owing to the diversity of techniques offered by CE, this method can be used to determine contents of both the high-molecular compounds, such as proteins or DNA fragments, and the low-molecular compounds: amino acids, carbohydrates, vitamins, flavonoids, inorganic ions, and organic acids. The use of this method and its techniques in food control laboratories can essentially facilitate the verification of the declared and real composition of food products.

Słowa kluczowe

PL

zywnosc; analiza zywnosci; metody analizy; elektroforeza kapilarna; wykorzystanie; bialko; fragmenty DNA; aminokwasy; weglowodany; witaminy; flawonoidy; kwasy organiczne; oznaczanie

• Wydawca: -
• Czasopismo: Żywność Nauka Technologia Jakość
• Rocznik: 2008
• Tom: 15
• Numer: 2
• Opis fizyczny: s.5-14,rys.,bibliogr.

Twórcy

autor

Cieslik E

• Małopolskie Centrum Monitoringu i Atestacji Żywności, Wydz. Technologii Żywności, Uniwersytet Rolniczy w Krakowie, ul. Balicka 122, 30-149 Kraków

autor

Niedospial A

• Małopolskie Centrum Monitoringu i Atestacji Żywności, Wydz. Technologii Żywności, Uniwersytet Rolniczy w Krakowie, ul. Balicka 122, 30-149 Kraków

autor

Mickowska B

• Małopolskie Centrum Monitoringu i Atestacji Żywności, Wydz. Technologii Żywności, Uniwersytet Rolniczy w Krakowie, ul. Balicka 122, 30-149 Kraków

Bibliografia

• [1] Ackermans M.T., Ackermans- Loonen J.C.J.M., Beckers J.L.: Determination of propionian in bread using capillary zone electrophoresis. J. Chrom., 1992, 627, 273-279.
• [2] Altria K., D.(red.): Capillary Electrophoresis Guidebook Principles, Operation, and Applications. Ed. Humana Press, New Jork 1996.
• [3] Arentoft A.M., Michaelsen S., Sørensen H.: Determination of oligosaccharides by capillary zone electrophoresis. J. Chrom. A, 1993, 652, 517-524.
• [4] Aurora-Prado M.S., Silva C. A., Tavares M.F.M., Altria K. D.: Determination of folic acid in tablets by microemulsion electrokinetic chromatography. J. Chrom. A, 2004, 1051, 291-296.
• [5] Avdalovic N., Poh, C.A., Rocklin R.D., Stillian J.R.: Determination of cations and anions by capillary electrophoresis combined with suppressed conductivity detection. Anal. Chem., 1993, 65, 1470-1475.
• [6] Bietz J.A., Schmatzried E.: Capillary electrophoresis of wheat gliadin: initial studies and application to varietal identification. Lebensm.-Wiss. U.-Technol., 1995, 28, 174-184.
• [7] Bjergegaard C., Michaelsen S., Mortensen K., Sørensen H.: Determination of flavonoids by micellar electrokinetic capillary chromatography. J. Chrom. A, 1993, 652, 77-85.
• [8] Bustamante-Rangel M., Delgado-Zamarreño M.M., Sánchez-Pérez A., Carabias-Martínez R.: Microemulsion electrokinetic chromatography for the separation of retinol, cholecalciferol, δ- tocopherol and α–tocopherol. J. Chrom. A, 2006, 1125, 270-273.
• [9] Cao Y., Chu Q., Fang Y., Ye J.: Analysis of flavonoids in Ginkgo biloba L. and its phytopharmaceuticals by capillary electrophoresis with electrochemical detection. Anal. Bioanal. Chem., 2002, 374, 294-299.
• [10] Cao Y.H., Wang Y., Yuan Q.: Analysis of flavonoids and phenolic acid in propolis by capillary electrophoresis. Chrom., 2004, 59, 135-140.
• [11] Chen F. T. A. & Tusak A.: Charakterization of food proteins by capillary electrophoresis. J. Chrom. A, 1994, 685, 331-337.
• [12] Chung S.-Y., Ullah A.H.J., Sanders T.H.: Peptide mapping of peanut proteins: identification of peptides as potential indicators of peanut maturity. J. Agric. Food Chem., 1994, 42, 623-628.
• [13] Cifuentes A., de Frutos M., Díez- Masa J. C.: Polymeric neworks vs cross-linked polyacrylamide bonded gels for CE separations of whey proteins. Am. Lab., 1994, 26, 46-51.
• [14] Colón L.A., Dadoo R., Zar R.N.: Determination of carbohydrates by capillary zone electrophoresis with amperometic detection at a copper microelectrode. Anal. Chem., 1993, 65, 476-481.
• [15] Cortacero-Ramirez S., Segura-Carretero A., Cruces-Blanco C., Romero-Romero M.L., Fernandez-Gutierrez A.: Simultaneous determination of multiple constituents in real beer samples of different origins by capillary zone electrophoresis. Anal. Bioanal. Chem., 2004, 380, 831-837.
• [16] Delago C., Tomás-Barberán F.A., Talou T., Gaset A.: Capillary electrophoresis as an alternative to HPLC for the determination of honey flavonoids. Chrom., 1994, 38, 71-78.
• [17] DeViries K. J.: Determination of organic acid in beer by capillary electrophoresis. J. Am. Soc. Brew. Chem., 1993, 51, 155-157.
• [18] Fotsing L., Fillet M., Bechet I., Hubert Ph., Crommen J.: Determination of six water-soluble vitamins in a pharmaceutical formulation by capillary electrophoresis. J. Pharm. Biomed. Anal., 1997, 15, 1113-1123.
• [19] Fukushi K., Takeda S., Wakida S., Higashi K., Hiiro K.: Determination of free calcium in vegetables by capillary zone electrophoresis. J. Chrom. A, 1997, 759, 211-216.
• [20] Fukushi K., Takeda S., Wakida S.-I., Yamane M., Higashi K., Hiiro K.: Determination of ascorbic acid in vegetables by capillary zone electrophoresis. J. Chrom. A, 1997, 772, 313-320.
• [21] García-Cañas V., González R., Cifuentes A.: Detection of genetically modified maize by the Polymerase Chain Reaction and Capillary Gel Electrophoresis with UV detection and Laser-Induced Fluorescence. J. Agric. Food Chem., 2002, 50, 1016-1021.
• [22] García-Cañas V., González R., Cifuentes A.: Highly reproducible Capillary Gel Electrophoresis (CGE) of DNA fragments using uncoated columns. Detection of genetically modified maize by PCR-CGE. J. Sep. Sci., 2002, 25, 577-583.
• [23] Giovannoli C., Anfossi L., Tozzi C., Giraudi G., Vanni A.: DNA separation by capillary electrophoresis with hydrophilic substituted celluloses as coating and sieving polymers. Application to the analysis of genetically modified meals. J. Sep. Sci., 2004, 27, 1551-1556.
• [24] Hilhorst M.J., Somsen G.W., de Jong G.J.: Potential of capillary electrophoresis for the profiling of propolis. J. High Resol. Chrom., 1998, 21, 608-612.
• [25] Horie H., Yamauchi Y., Kohata K.: Analysis of organic anions in tea infusions using capillary electrophoresis. J. Chrom. A, 1998, 817, 139-144.
• [26] Hu Q., Zhou T., Zhang L., Li H., Fang Y.: Separation and determination of three water-soluble vitamins in pharmaceutical preparations and food by micellar electrokinetic chromatography with amperometric electrochemical detection. Anal. Chem. Acta., 2001, 437, 123-129.
• [27] Jimidar M., Hartmann C., Cousement N., Massart D.L.: Determination of nitrate and nitrite in vegetables by capillary electrophoresis with indirect injection. J. Chrom. A., 1995, 706, 479-492.
• [28] Jones W.R., Jandik P.: New methods for chromatographic separations of anions. Am Lab, 1990, 22, 54-64.
• [29] Kajiwara H., Sato A., Kaneko S.: Analysis of calcium and magnesium ions in wheat flour by capillary zone electrophoresis. Biosci. Biotech. Biochem., 1993, 57, 1010-1011.
• [30] Kandl T., Kupina S.: An improved capillary electrophoresis procedure for the determination of organic acids in grape juice and wine. Am. J. Enol. Vitic., 1999, 50, 155-161.
• [31] Kanning M., Casella M. & Olieman C.: Milk and soy proteins analysis using capillary zone electrophoresis. LC·GC Int., 1993, 6, 701-706.
• [32] Kenny B. F.: Determination of organic acid in food samples by capillary electrophoresis. J. Chrom., 1991, 546, 423-430.
• [33] Klampfl Ch. W., Buchberger W., Turner M., Fritz J.S.: Determination of underivatized amino acids in beverage samples by capillary electrophoresis. J. Chrom. A, 1998, 804, 349-355.
• [34] Kuban P., Karlberg B.: Simultaneous Determination of small cations and anions by Capillary Electrophoresis. Anal. Chem., 1998, 70, 360-365.
• [35] Lalljie S.P.D., Vindevoge J., Sandra P.: Quantitation of organic acid in sugar refinery juices with capillary zone electrophoresis and indirect UV detection. J. Chrom. A, 1993, 652, 563-569.
• [36] Li K. & Li S.F.Y.: Determination of anions in water samples by capillary zone electrophoresis with indirect UV detection. J. Liq. Chrom., 1994, 17, 3889-3910.
• [37] Lin-Chau C., Huan-Tsung Ch., Shao-Wen, S.: Cyclodextrin-modified microemulsion electrokinetic chromatography for separation of α-, γ-, δ-tocopherol and α-tocopherol acetate. J. Chrom. A, 2006, 1110, 227-234.
• [38] Liu W., Chen G., Cui T.: Determination of flavones in crataegus pinnatifida by Capillary Zone Electrophoresis. J. Chrom., 2003, 41, 87-91.
• [39] Lookhart G.L., Bean S.R.: Ultrafast CE analysis of cereal storage proteins and its applications to protein characterization and cultivar differentiation. J. Agric. Food Chem., 2000, 48, 344-353.
• [40] Marshall P.A., Trenerry V.C.: The determination of nitrite and nitrate in foods by capillary ion electrophoresis. Food Chem., 1996, 57, 339-345.
• [41] Morin P., Francois C., Dreux M.: Capillary electrophoresis of alkali and alkaline-earth cations with imidazole or benzylamine buffers. J. Liq. Chrom., 1994, 17, 3869-3888.
• [42] Nouadje G., Couderc F., Puig Ph., Hernandez L.: Combination of micellar electrokinetic chromatography and laser-induced fluorescence detection for the determination of presser amines and some principal amine acids in wine. J. Cap Electrophoresis, 1995, 2, 117.
• [43] Otte J.A.H.J., Kristiansen K.R., Zakora M., Qvist K.B.: Separation of individual whey proteins and measurement of α-lactaalbumin, β-lactoglobulin by capillary zone electrophoresis. Neth Milk Dairy J., 1994, 48, 81-97.
• [44] Öztekin N., Bedia Erim F.: Simultaneous determination of inorganic anions and organic acids by Capillary Electrophoresis. Turk. J. Chem., 2001, 25, 145-150.
• [45] Righetti P.G., Oliveri E., Viotti A.: Identification of maize lines via capillary electrophoresis of zeins in isoelectric, acidic buffers. Electrophoresis, 1998, 19, 1738-1741.
• [47] Shihabi Z.K., Kute T.L., Hinsdale M.: Analysis of isoflavones by capillary electrophoresis. J. Chrom. A, 1994, 680, 181-185.
• [46] Schiewe J., Mrestani Y., Neubert R.: Application and optimization of capillary zone electrophoresis in vitamin analysis. J. Chrom. A, 1995, 717, 255-259.
• [48] Swallow K.W.: Capillary zone electrophoretic analysis of the minor anions present in orange juice and orange pulpwash. J. Agric. Food Chem., 1994, 42, 2808-2811.
• [49] Tienstra P., van Riel J.A.M., Olieman C.: Determination of goat milk in cow milk with P/ACE capillary electrophoresis. Technical Information DS-832, Beckman Instruments, Inc., 1992b, Fullerton, CA, USA.
• [50] Thompson Catherine O., Trenerry V.C.: A rapid method for the determination of total L-ascorbic acid in fruits and vegetables by micellar electrokinetic capillary chromatography. Food Chem., 1995, 53, 43-50.
• [51] Trevaskis M., Trenerry V.C.: An investigation into the determination of oxalic acid in vegetables by capillary electrophoresis. Food Chem., 1996, 57, 323-330.
• [52] Vorndran A.E., Oefner P.J., Scherz H., Bonn G.K.: Indirect UV detection of carbohydrates in capillary zone electrophoresis. Chrom., 1992, 33, 163-168.
• [53] Ward C.M., Trenerry V.C.: The determination of niacin in cereals, meat and selected foods by capillary electrophoresis and high performance liquid chromatography. Food Chem., 1997, 60, 667-674.
• [54] Ward C.M. Trenerry V.C., Pant I.: The application of capillary electrophoresis to the determination of total niacin in concentrated yeast spreads. Food Chem., 1997, 58, 185-192.
• [55] Witkiewicz Z.: Podstawy chromatografii. WNT, Warszawa 2005.