Evaluation of the virucidal activity of Iris flavissima Pall

• Autorzy: Rajtar B , Machalska A. , Jakubiec A. , Polz-Dacewicz M. , Otgonbaatar U. , Dungerdorj D.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was the evaluation of the cytotoxicity and virucidal activity of the extracts from underground parts of Iris flavissima Pall. The plant material was extracted using: methanol/H₂O (1:1 v/v) and methanol. The examination of the cytotoxicity of the extracts in the concentration of 0.025; 0.05; 0.1; 0.25; 0.5; 1; 2, and 5 mg mL⁻¹ was carried out on GMK cell cultures. The cultures were incubated at 37°C in an atmosphere with 5% of CO₂ for 24-72 h. The cytotoxicity was measured by the colorimetric MTT (tetrazolium) method. Human enterovirus ECHO 9 was used for the evaluation of the virucidal activity of the extracts. The suspension of the virus was mixed (1:1 v/v) with the examined extracts at the concentrations non-toxic to GMK cell cultures and incubated at 37°C for 1 h, and then the virus was titrated in the GMK cell culture. The titre of the virus was estimated according to the Reed-Muench method. The methanolic and methanol/H₂O extracts were found to be non-toxic to GMK cells at the concentration of 1 mg ml⁻¹ and 2 mg ml⁻¹, respectively. The virucidal effect was observed for the both investigated extracts. The methanolic extract decreased ECHO 9 replication by 1.46 log, and methanol/H₂O extract - by 2.08 log.

Słowa kluczowe

EN

plant extract; virucidal activity; Iris flavissima; Iridaceae; plant material; human enterovirus; cytotoxicity

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 3
• Opis fizyczny: p.337-339,ref.

Twórcy

autor

Rajtar B

• Medical University of Lublin, 20-093 Lublin, Poland

autor

Machalska A.

autor

Jakubiec A.

autor

Polz-Dacewicz M.

autor

Otgonbaatar U.

autor

Dungerdorj D.

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