Heavy metals-induced hydrogen peroxide production in tobacoo cells

• Autorzy: Zrobek-Sokolnik A , Gorska K. , Gorecki R.J.

Warianty tytułu

PL

Produkcja nadtlenku wodoru w komorkach tytoniu pod wplywem metali ciezkich

• Języki publikacji: EN

Abstrakty

EN

n this work the reaction of Nicotiana tabacum cv. Bright Yellow 2 cells on the presence of 500 μM zinc, 500 μM cadmium, 10 μM copper and 5 μM iron was studied. It was confirmed that all tested heavy metals induced in tobacco cell suspension culture early stress response manifested by increased production of hydrogen peroxide. The highest level of hydrogen peroxide was marked in samples containing cadmium ions. This metal was also the most toxic for tested plant material. Less toxic were ions of copper and zinc. Iron ions gave the weakest stress response (probably because of the plant cell peroxidase inhibition).

PL

W pracy badano reakcje komórek Nicotiana tabacum cv. Bright Yellow 2 na obecność 500 μM cynku, 500 μM kadmu, 10 μM miedzi i 5 μM żelaza. Stwierdzono, że wszystkie testowane metale ciężkie indukowały w zawiesinie tytoniu wczesną odpowiedź stresową, manifestującą się zwiększoną produkcją H₂O₂. Najwyższy poziom nadtlenku wodoru oznaczono w próbach zawierających jony kadmu. Metal ten był również najbardziej toksyczny dla materiału badawczego. Mniejszą toksyczność przejawiały jony miedzi i cynku, najsłabszą – jony żelaza. Było to, najprawdopodobniej, efektem hamującego oddziaływania jonów żelaza na aktywność peroksydaz ścian komórkowych.

• Wydawca: -
• Czasopismo: Polish Journal of Natural Sciences
• Rocznik: 2007
• Tom: 22
• Numer: 2
• Opis fizyczny: p.196-203,fig.,ref.

Twórcy

autor

Zrobek-Sokolnik A

• University of Warmia and Mazury in Olsztyn, pl.Lodzki 1, 10-727 Olsztyn, Poland

autor

Gorska K.

autor

Gorecki R.J.

Bibliografia

• ALI G., SRIVASTAVA P.S., IQBAL M. 1999. Morphogenic and biochemical responses of Bacopa monniera cultures to zinc toxicity. Plant Sci., 143: 187-193.
• APOSTOL J., HINSTEIN P.F., LOW P.S. 1989. Rapid stimulation of an oxidative burst during elicitation of cultured plant cells. Role in defense and signal transduction. Plant Physiol., 90: 109-116.
• BRIAT J.F., LEBRUN M. 1999. Plant response to metal toxicity. Life Sci., 322: 43-54.
• BROWN G.E., FOSTER A.L., OSTERGERN J.H. 1999. Mineral surfaces and bioavailability to heavy metals: A molecular-scale perspective. PNAS, 96: 3388-3395.
• CHEN L.-M., LIN C.C., KAO C.H. 2000. Copper toxicity in rice seedlings: Changes in antioxidative enzyme activities, H₂O₂ level, and cell wall peroxidase activity in roots. Bot. Bull. Acad. Sin., 41: 99-103.
• CUYPERS A., VANGRONSVELD J., CLIJSTERS H. 1999. The chemical behavior of heavy metals plays a prominent role in the induction of oxidative stress. Free Rad. Res., 31: 39-43.
• CUYPERS A., VANGRONSVELD J., CLIJSTERS H. 2001. The redox status of plant cells (AsA and GSH) is sensitive to zinc imposed oxidative stress in roots and primary leaves of Phaseolus vulgaris. Plant Physiol. Biochem., 39: 657-664.
• FANG W.C., KAO C.H. 2000. Enhanced peroxidase activity in rice leaves in response to excess iron, copper and zinc. Plant Sci., 158: 71-76.
• GESTELEN VAN P., LADEGANCK P., WAYNANT I., CAUBERGES R.J., ASARD H. 1998. The cantharidin-induced oxidative burst in tobacco BY-2 cell suspension cultures. Protoplasma, 205: 83-92.
• GWÓŹDŹ E.A., PRZYMUSIŃSKI R., RUCIŃSKA R., DECKERT J. 1997. Plant cell responses to heavy metals: molecular and physiological aspects. Acta Physiol. Plant., 19: 459-465.
• KAWANO T., KAWANO N., MUTO S., LAPERYRIE F. 2002. Retardation and inhibition of the cation-induced superoxide generation in BY-2 tobacco cell suspension culture by Zn²⁺ and Mn²⁺. Physiol. Plant., 114: 395-404.
• LOMBARDI L., SEBASTIANI L. 2005. Copper toxicity in Prunus cerasifera: growth and antioxidant enzymes responses of in vitro grown plants. Plant Sci., 168: 797-802.
• NAGATA T., NEMOTO Y., HASEZAWA S. 1992. Tobacco BY-2 cell line as the “HeLa” cell in the cell biology of higher plants. Int. Rev. Cytol., 132: 1-30.
• MITHÖFER A., SCHULTZE B., BOLAND W. 2004. Biotic and heavy metal stress response in plants: evidence for common signals. FEBS Lett., 566: 1-5.
• MURASHIGE T., SKOOG F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant., 15: 437-497.
• OLMOS E., MARTINEZ-SOLANO J.R., PIQUERAS A., HELLIN E. 2003. Early steps in the oxidative burst induced by cadmium in cultured tobacco cells (BY-2 line). J. Exp. Bot., 381: 291-301.
• PIQUERAS A., OLMOS E., MARTINEZ-SOLANO J.R., HELLIN E. 1999. Cd-induced oxidative burst in tobacco BY-2 cells: time course, subcellular location and antioxidant response. Rev. Rad. Res., 31: 933-938.
• RAEYMAEKERS T., POTTERS G., ASARD H., GUISEZ Y., HOREMANS N. 2003. Copper-mediated oxidative burst in Nicotiana tabacum L. cv. Bright Yellow 2 cell suspension cultures. Protoplasma, 221: 93-100.
• REICHHELD J., VERMOUX T., LARDON F., VAN MONTAGU M., INZE¨ D. 1999, Specific checkpoints regulate plant cell cycle progression in response to oxidative stress. Plant J., 17: 101-108.
• SANITA DI TOPPI L., GABBRIELLI R. 1999. Response to cadmium in higher plants. Environ. Exp. Bot., 41: 105-130.
• SHU¨ TZENDU¨ BEL A., POLLE A. 2002. Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrihzation. J. Exp. Bot., 53: 1351-1365.
• SOBKOWIAK R., RYMER K., RUCIŃSKA R., DECKERT J., GWÓŹDŹ E.A. 2004. Cdamium-induced changes in antioxidant enzymem in suspension culture cells of soybean. Acta Biochim. Pol., 51: 219-222.
• URBAŃSKI N.K., BERĘSEWICZ A. 2000. Generation of ·OH initiated by interaction of Fe²⁺ and Cu+ with dioxygen; comparison with the Fenton chemistry. Acta Biochim. Pol., 47: 951-962.
• WANG Y., FANG J., STEPHEN L.S., RAO K., MURALI K. 2004. Cadmium inhibits the electron transfer chain and induces reactive oxygen species. Free Rad. Biol. Med., 36: 1434-1443.
• WOJTASZEK P. 1997. Oxidative burst: an early plant response to pathogen infection. Biochem. J., 322: 681-692.
• YAMAMOTO Y., HACHIYA A., MATSUMOTO H. 1997. Oxidative damage to membranes by a combination of aluminum and iron in suspension-cultured tobacco cells. Plant Cell Physiol., 38: 1333-1339.