The influence of PPM upon the sterility of the in vitro cultures in European beech [Fagus sylvatica L.]

• Autorzy: Kraj W. , Dolnicki A.
• Języki publikacji: EN

Abstrakty

EN

Investigations were conducted on the strongly infested by microbes embryos isolated from seed stratified in non-sterile conditions or from tree buds. The results showed, that PPM (Plant Preservative Mixture, produced by Plant Cell Technology, Inc.) was very useful for increasing the percentage of sterile cultures disinfected in a 5% solution of NaOCl. The best way of applying PPM turned out to be adding the substance directly to the growing medium. At the concentration of 2 or 4 cm3 x dm-3 of PPM there was an 30% increase in percentage of sterile of in vitro cultures developed from embryos and 70-80% of cultures developed from buds, as compared with the control without PPM. At the range of concentrations employed in this study, no negative effects of PPM upon the development and growth of callus were observed.

PL

Słowa kluczowe

EN

Plant Preservative Mixture; plant physiology; tissue culture; tree bud; Fagus sylvatica; sterilization; beech; sterility; in vitro; embryo; European beech; bud

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2003
• Tom: 72
• Numer: 4
• Opis fizyczny: p.303-307,ref.

Twórcy

autor

Kraj W.

• Agricultural University, Al.29 Listopada 46, 31-425 Krakow, Poland

autor

Dolnicki A.